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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
31/07/2014 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KILAMBO, D. L; GUERRA-GUIMARÃES, L.; MABAGALA, R. B.; VARZEA, V. M. P.; HADDAD, F.; LOUREIRO, A.; TERI, J. M. |
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Afiliação: |
D. L. KILAMBO, Tanzania Coffee Research Institute; L. GUERRA-GUIMARÃES, Instituto de Investigação Científica Tropical; R. B. MABAGALA, Sokoine University of Agriculture; V. M. P. VARZEA, Instituto de investigação Científica Tropical; FERNANDO HADDAD, CNPMF; LOUREIRO A., Instituto de investigação Científica Tropical; J. M. TERI, Tanzania Coffee Research Institute. |
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Título: |
Characterization of Colletotrichum kahawae strains in Tanzania. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
International Journal of Microbiology Research, v. 5, Issue 2, 2013. |
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ISSN: |
0975-5276 |
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Idioma: |
Inglês |
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Conteúdo: |
Strains of Colletotrichum kahawae from different coffee growing areas in Tanzania, Kenya and Cameroon were studied for their different characters. Characterization was done through the application of iso-enzyme whereby morphological attributes were studied to determine variability of C. kahawae. Application of iso-enzymes involved extraction of proteins from the mycelia of each of the strain and the content was determined using Bio-Rad protein assay kit. The strains were Tanzania (14), Kenya (2) and Cameroon (1), and Colletotrichum gloeosporioides. The protein content of C. kahawae strains were compared for their reaction to iso-enzymes based on the activity of esterace, acid and basic phosphotase using the technique of isoelectic focusing electrophoresis (IEF). It was possible to detect a high esterase activity portraying many bands per strain of C. kahawae, but lower in the case of basic and acid phosphatase. However all enzymes revealed polymorphisms. The data matrices from enzymes pattern were formed by identifying the presence or absence of bands. A phenogram based on the estimate similarity coefficients was constructed by un-weighed pair group methods (UPGMA) using the computer software package NTSYS-PC version 2.02. The original similarity matrix was compared with the cophenetic value matrix generated from the systems of clusters. The cophenetic coefficient (r) was estimated and used as a measure of the goodness of fit. The results obtained by cluster analysis on C. kahawaeCam1 strain shows that iso-enzymatic profile of the strain is different from the rest. In the Tanzania population studied two groups were formed and C. kahawae strain T3 seemed to be different from the rest of the strain. Characteristics of Colletotrichum strains collected from different coffee growing areas were observed after 10 days of growth on MEA in darkness at temperature ranges between 15°C to 30°C. The morph cultural characteristics used to study variability of Colletotrichum spp were cultural texture, conidia shape and sizes, and growth rate. Out of 30 Colletotrichum spp isolated from diseased green coffee berries, 25 isolates were identified as Colletotrichum kahawae producing dark grey cottony, oval conidia morphology and slow growth rate. Five isolates identified as Colletotrichum gloeosporioides; growth rate ranged from 7.3 to 8.8 mm and C. kahawae ranged from 5.0 to 5.5 mm per 24 hr. at 25oC. This shows that the growth rate of C. gloeosporioides was always more rapid than that of C. kahawae. This shows that the growth rate analysis is a useful method separating C. kahawae from C. gloeosporioides. Conidia sizes of C. kahawaestrains were variable even within one strain. However based on this study, variability in spore size can not be used to distinguish the strains of C. kahawae. MenosStrains of Colletotrichum kahawae from different coffee growing areas in Tanzania, Kenya and Cameroon were studied for their different characters. Characterization was done through the application of iso-enzyme whereby morphological attributes were studied to determine variability of C. kahawae. Application of iso-enzymes involved extraction of proteins from the mycelia of each of the strain and the content was determined using Bio-Rad protein assay kit. The strains were Tanzania (14), Kenya (2) and Cameroon (1), and Colletotrichum gloeosporioides. The protein content of C. kahawae strains were compared for their reaction to iso-enzymes based on the activity of esterace, acid and basic phosphotase using the technique of isoelectic focusing electrophoresis (IEF). It was possible to detect a high esterase activity portraying many bands per strain of C. kahawae, but lower in the case of basic and acid phosphatase. However all enzymes revealed polymorphisms. The data matrices from enzymes pattern were formed by identifying the presence or absence of bands. A phenogram based on the estimate similarity coefficients was constructed by un-weighed pair group methods (UPGMA) using the computer software package NTSYS-PC version 2.02. The original similarity matrix was compared with the cophenetic value matrix generated from the systems of clusters. The cophenetic coefficient (r) was estimated and used as a measure of the goodness of fit. The results obtained by cluster analysis on C. k... Mostrar Tudo |
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Thesaurus Nal: |
Colletotrichum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03447naa a2200217 a 4500
001 1991623
005 2023-05-25
008 2013 bl uuuu u00u1 u #d
022 $a0975-5276
100 1 $aKILAMBO, D. L
245 $aCharacterization of Colletotrichum kahawae strains in Tanzania.$h[electronic resource]
260 $c2013
520 $aStrains of Colletotrichum kahawae from different coffee growing areas in Tanzania, Kenya and Cameroon were studied for their different characters. Characterization was done through the application of iso-enzyme whereby morphological attributes were studied to determine variability of C. kahawae. Application of iso-enzymes involved extraction of proteins from the mycelia of each of the strain and the content was determined using Bio-Rad protein assay kit. The strains were Tanzania (14), Kenya (2) and Cameroon (1), and Colletotrichum gloeosporioides. The protein content of C. kahawae strains were compared for their reaction to iso-enzymes based on the activity of esterace, acid and basic phosphotase using the technique of isoelectic focusing electrophoresis (IEF). It was possible to detect a high esterase activity portraying many bands per strain of C. kahawae, but lower in the case of basic and acid phosphatase. However all enzymes revealed polymorphisms. The data matrices from enzymes pattern were formed by identifying the presence or absence of bands. A phenogram based on the estimate similarity coefficients was constructed by un-weighed pair group methods (UPGMA) using the computer software package NTSYS-PC version 2.02. The original similarity matrix was compared with the cophenetic value matrix generated from the systems of clusters. The cophenetic coefficient (r) was estimated and used as a measure of the goodness of fit. The results obtained by cluster analysis on C. kahawaeCam1 strain shows that iso-enzymatic profile of the strain is different from the rest. In the Tanzania population studied two groups were formed and C. kahawae strain T3 seemed to be different from the rest of the strain. Characteristics of Colletotrichum strains collected from different coffee growing areas were observed after 10 days of growth on MEA in darkness at temperature ranges between 15°C to 30°C. The morph cultural characteristics used to study variability of Colletotrichum spp were cultural texture, conidia shape and sizes, and growth rate. Out of 30 Colletotrichum spp isolated from diseased green coffee berries, 25 isolates were identified as Colletotrichum kahawae producing dark grey cottony, oval conidia morphology and slow growth rate. Five isolates identified as Colletotrichum gloeosporioides; growth rate ranged from 7.3 to 8.8 mm and C. kahawae ranged from 5.0 to 5.5 mm per 24 hr. at 25oC. This shows that the growth rate of C. gloeosporioides was always more rapid than that of C. kahawae. This shows that the growth rate analysis is a useful method separating C. kahawae from C. gloeosporioides. Conidia sizes of C. kahawaestrains were variable even within one strain. However based on this study, variability in spore size can not be used to distinguish the strains of C. kahawae.
650 $aColletotrichum
700 1 $aGUERRA-GUIMARÃES, L.
700 1 $aMABAGALA, R. B.
700 1 $aVARZEA, V. M. P.
700 1 $aHADDAD, F.
700 1 $aLOUREIRO, A.
700 1 $aTERI, J. M.
773 $tInternational Journal of Microbiology Research$gv. 5, Issue 2, 2013.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
19/12/2023 |
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Data da última atualização: |
19/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MOTA, M. C.; CANDIDO, L. A.; CUADRA, S. V.; MARENCO, R. A.; SOUZA, R. V. A. de; TOMÉ, A. M.; LOPES, A. B. de A.; LIMA, F. L. de; REIS, J.; BRIZOLLA, R. M. |
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Afiliação: |
MARCELO CRESTANI MOTA, FACULDADE MARECHAL RONDON; LUIZ ANTONIO CANDIDO, INSTITUTO NACIONAL DE PESQUISAS DA AMAZÔNIA; SANTIAGO VIANNA CUADRA, CNPTIA; RICARDO ANTONIO MARENCO, INSTITUTO NACIONAL DE PESQUISAS DA AMAZÔNIA; RITA VALÉRIA ANDREOLI DE SOUZA, UNIVERSIDADE DO ESTADO DO AMAZONAS; ADRIANO MAITO TOMÉ, FACULDADE MARECHAL RONDON; ANDRESSA BACK DE ANDRADE LOPES, FACULDADE MARECHAL RONDON; FRANCINEI LOPES DE LIMA, FACULDADE MARECHAL RONDON; JULIANA REIS, FACULDADE MARECHAL RONDON; RAFAEL MORBEQUE BRIZOLLA, FACULDADE MARECHAL RONDON. |
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Título: |
CROPGRO-soybean model – Validation and application for the southern Amazon, Brazil. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Computers and Electronics in Agriculture, v. 216, 108478, Jan. 2024. |
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DOI: |
https://doi.org/10.1016/j.compag.2023.108478 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective in this work was to calibrate and validate the CROPGRO-Soybean model with soybean data collected in harvests of 2017 to 2019, being the data from the first growing period devoted to model calibration, and crop data from 2018/2019 to model validation. |
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Palavras-Chave: |
Coeficientes genéticos; Crop simulation; Ferramenta de pesquisa; Genetic coefficients; Índice de área foliar; Manejo de colheita; Modelagem; Research tool; Simulação de colheita. |
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Thesagro: |
Glycine Max; Soja. |
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Thesaurus NAL: |
Crop management; Leaf area index; Models. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01462naa a2200409 a 4500
001 2159999
005 2024-01-19
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.compag.2023.108478$2DOI
100 1 $aMOTA, M. C.
245 $aCROPGRO-soybean model – Validation and application for the southern Amazon, Brazil.$h[electronic resource]
260 $c2024
520 $aThe objective in this work was to calibrate and validate the CROPGRO-Soybean model with soybean data collected in harvests of 2017 to 2019, being the data from the first growing period devoted to model calibration, and crop data from 2018/2019 to model validation.
650 $aCrop management
650 $aLeaf area index
650 $aModels
650 $aGlycine Max
650 $aSoja
653 $aCoeficientes genéticos
653 $aCrop simulation
653 $aFerramenta de pesquisa
653 $aGenetic coefficients
653 $aÍndice de área foliar
653 $aManejo de colheita
653 $aModelagem
653 $aResearch tool
653 $aSimulação de colheita
700 1 $aCANDIDO, L. A.
700 1 $aCUADRA, S. V.
700 1 $aMARENCO, R. A.
700 1 $aSOUZA, R. V. A. de
700 1 $aTOMÉ, A. M.
700 1 $aLOPES, A. B. de A.
700 1 $aLIMA, F. L. de
700 1 $aREIS, J.
700 1 $aBRIZOLLA, R. M.
773 $tComputers and Electronics in Agriculture$gv. 216, 108478, Jan. 2024.
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Embrapa Agricultura Digital (CNPTIA) |
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