| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
10/07/2012 |
|
Data da última atualização: |
04/04/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SANTOS, R. F.; OLIVEIRA, C. F.; VARÉA, G. S.; SILVA, M. L. C. ORRADI da; IDA, E. I.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C.; RIBEIRO, M. L. L. |
|
Afiliação: |
R. F. SANTOS, UEL; C. F. OLIVEIRA, UEL; G. S. VARÉA, UEL; M. L. C. ORRADI DA SILVA, UNESP Presidente Prudente; ELZA I. IDA, UEL; JOSE MARCOS GONTIJO MANDARINO, CNPSO; MERCEDES CONCORDIA CARRAO PANIZZI, CNPSo; M. L. L. RIBEIRO, UEL. |
|
Título: |
Purification and characterization of soy cotyledon B-glucosidase. |
|
Ano de publicação: |
2013 |
|
Fonte/Imprenta: |
Journal of Food Biochemistry, v. 37, n. 3, p. 302-312, Jun. 2013. |
|
DOI: |
10.1111/j.1745-4514.2011.00632.x |
|
Idioma: |
Inglês |
|
Conteúdo: |
b-Glucosidase F42 of soy cotyledons was purified by ammonium sulfate fractionation, ion-exchange chromatography (CM-Sephadex-C-50, Sigma, St. Louis, MO) and gel filtration (Sephadex G-100, Sigma). The enzyme was purified 111.8-fold relative to its concentration in the crude extract. It had an apparent molecular mass of 53 kDa in gel filtration experiments and produced a 33-kDa band in sodium dodecyl sulfate?polyacrylamide gel electrophoresis, suggesting that it is dimeric. The purified b-glucosidase F42 was characterized as a glycoprotein after the identification of fucose, galactosamine and glucosamine by high-pressure anion-exchange chromatography?pulsed amperometric detector. Its highest activity was observed at pH 5.0 and 45C, and it was stable for up to 4 days at 25C. The Km of the enzyme was 0.12 mMp-nitrophenyl-b-d-glucopyranoside. b-Glucosidase F42 showed specificity for different substrates, and its activity was inhibited by 1 mM HgCl2, 10mM glucono-d-lactone or 150 mMglucose and increased by 10 mMMnCl2. |
|
Palavras-Chave: |
Soybean. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01723naa a2200229 a 4500
001 1928015
005 2022-04-04
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1745-4514.2011.00632.x$2DOI
100 1 $aSANTOS, R. F.
245 $aPurification and characterization of soy cotyledon B-glucosidase.$h[electronic resource]
260 $c2013
520 $ab-Glucosidase F42 of soy cotyledons was purified by ammonium sulfate fractionation, ion-exchange chromatography (CM-Sephadex-C-50, Sigma, St. Louis, MO) and gel filtration (Sephadex G-100, Sigma). The enzyme was purified 111.8-fold relative to its concentration in the crude extract. It had an apparent molecular mass of 53 kDa in gel filtration experiments and produced a 33-kDa band in sodium dodecyl sulfate?polyacrylamide gel electrophoresis, suggesting that it is dimeric. The purified b-glucosidase F42 was characterized as a glycoprotein after the identification of fucose, galactosamine and glucosamine by high-pressure anion-exchange chromatography?pulsed amperometric detector. Its highest activity was observed at pH 5.0 and 45C, and it was stable for up to 4 days at 25C. The Km of the enzyme was 0.12 mMp-nitrophenyl-b-d-glucopyranoside. b-Glucosidase F42 showed specificity for different substrates, and its activity was inhibited by 1 mM HgCl2, 10mM glucono-d-lactone or 150 mMglucose and increased by 10 mMMnCl2.
653 $aSoybean
700 1 $aOLIVEIRA, C. F.
700 1 $aVARÉA, G. S.
700 1 $aSILVA, M. L. C. ORRADI da
700 1 $aIDA, E. I.
700 1 $aMANDARINO, J. M. G.
700 1 $aCARRÃO-PANIZZI, M. C.
700 1 $aRIBEIRO, M. L. L.
773 $tJournal of Food Biochemistry$gv. 37, n. 3, p. 302-312, Jun. 2013.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
20/02/2013 |
|
Data da última atualização: |
21/08/2017 |
|
Autoria: |
BRUGNARA, E. C.; BASTIANEL, M.; WEILER, R. L.; SCHWARZ, S. F. |
|
Afiliação: |
EDUARDO CESAR BRUGNARA, EMATER/SC; MARINÊS BASTIANEL, APTA; ROBERTO LUÍS WEILER, UFRGS; SERGIO FRANCISCO SCHWARZ, UFRGS. |
|
Título: |
Genetic similarity in a hybrid population of 'Montenegrina' and 'King' mandarins. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 8, p. 1103-1109, ago. 2012 |
|
Páginas: |
p.1103-1109 |
|
Idioma: |
Inglês |
|
Notas: |
Título em português: Similaridade genética em uma população de híbridos de tangerineiras 'Montenegrina' e 'King'. |
|
Conteúdo: |
The objective of this work was to confirm the hybrids obtained in plants originated from the crossing between the mandarins Citrus deliciosa 'Montenegrina' and C. nobilis 'King', and to estimate the genetic similarity among hybrids, and between each hybrid and its parents. Twenty?three pairs of microsatellite primers were tested. Fourteen of these pairs showed polymorphic bands between parents. Primers CCSM 129 and CCSME 52 were sufficient to identify the 12 nucellar clones observed in the studied population. Genetic similarity analysis of the population (hybrids and parents) showed 0.56 average similarity. Besides the 12 clones of 'Montenegrina' identified, 25 hybrids were found of which D18, C32, D06, C05 and D09 are the more similar to 'Montenegrina'. |
|
Palavras-Chave: |
Hybrid identification; Marcador microssatélite; Microsatellite markers. |
|
Thesaurus NAL: |
Citrus deliciosa; Citrus nobilis; Hybrids. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/76823/1/PAB-v.47-n.8-p.1103-1109.pdf
|
|
Marc: |
LEADER 01618naa a2200253 a 4500
001 1950236
005 2017-08-21
008 2012 bl uuuu u00u1 u #d
100 1 $aBRUGNARA, E. C.
245 $aGenetic similarity in a hybrid population of 'Montenegrina' and 'King' mandarins.
260 $c2012
300 $ap.1103-1109
500 $aTítulo em português: Similaridade genética em uma população de híbridos de tangerineiras 'Montenegrina' e 'King'.
520 $aThe objective of this work was to confirm the hybrids obtained in plants originated from the crossing between the mandarins Citrus deliciosa 'Montenegrina' and C. nobilis 'King', and to estimate the genetic similarity among hybrids, and between each hybrid and its parents. Twenty?three pairs of microsatellite primers were tested. Fourteen of these pairs showed polymorphic bands between parents. Primers CCSM 129 and CCSME 52 were sufficient to identify the 12 nucellar clones observed in the studied population. Genetic similarity analysis of the population (hybrids and parents) showed 0.56 average similarity. Besides the 12 clones of 'Montenegrina' identified, 25 hybrids were found of which D18, C32, D06, C05 and D09 are the more similar to 'Montenegrina'.
650 $aCitrus deliciosa
650 $aCitrus nobilis
650 $aHybrids
653 $aHybrid identification
653 $aMarcador microssatélite
653 $aMicrosatellite markers
700 1 $aBASTIANEL, M.
700 1 $aWEILER, R. L.
700 1 $aSCHWARZ, S. F.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 47, n. 8, p. 1103-1109, ago. 2012
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
