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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
26/06/2012 |
Data da última atualização: |
08/09/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
KESKINTEPE, L.; SIMPLICIO, A. A.; BRACKETT, B. G. |
Afiliação: |
Augusta Repro. Biology Associates, 812 Chafee Ave., United States; Aurino Alves Simplício, CNPC; Dept. of Physiol. and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, United States. |
Título: |
Caprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Theriogenology, New York, v. 49, n. 7, p. 1265-1274, May, 1998. |
DOI: |
10.1016/S0093-691X(98)00074-0 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The feasibility of using frozen-thawed semen in caprine IVF outside the breeding season was investigated. Electroejaculated spermatozoa from a Nubian buck were washed twice and then frozen in skim milk- or in egg yolk-based extenders. Goat oocytes were matured and inseminated by frozen-thawed spermatozoa selected by swim-up. In vitro fertilization was performed in a modified defined medium (mDM), altered experimentally, for 24 h. Embryos were cultured in 50 ?L of c-SOF+NEA for 9 d. The percentages of oocytes exposed to heparin-capacitated spermatozoa, (previously cryopreserved in skim milk- based extender) that cleaved, reached morula, blastocyst and expanded blastocyst stages were 82.8, 57.1, 35.7 and 30.0%, respectively. Without heparin treatment the rates for cleavage, morula, blastocyst and expanded blastocyst stages were 44.3, 31.4, 18.6 and 8.6%, respectively. Therefore, heparin treatment was included in sperm capacitation. Use of spermatozoa with BSA in the IVF medium yielded no cleavage. Although extenders containing 8 to 20% egg yolk enabled good sperm motility after cryopreservation, in vitro fertilizing ability was compromised under our conditions. By contrast, semen commercially processed in season in an egg yolk-based diluent remained effective for IVF. The highest proportion of blastocysts resulted from the use of spermatozoa diluted in a skim milk extender, heparin capacitation, and insemination in medium containing lamb serum. |
Palavras-Chave: |
Animal embryos; Blastocisto; Extenders; Ovary; Sperm cryopreservation; Sperm preservation. |
Thesagro: |
Caprino; Criopreservação; Espermatozóide; Inseminação artificial; Reprodução animal; Sêmen. |
Thesaurus Nal: |
Animal breeding; Blastocystis; Goats; In vitro fertilization; Reproduction. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02532naa a2200361 a 4500 001 1927208 005 2017-09-08 008 1998 bl uuuu u00u1 u #d 024 7 $a10.1016/S0093-691X(98)00074-0$2DOI 100 1 $aKESKINTEPE, L. 245 $aCaprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders.$h[electronic resource] 260 $c1998 520 $aAbstract: The feasibility of using frozen-thawed semen in caprine IVF outside the breeding season was investigated. Electroejaculated spermatozoa from a Nubian buck were washed twice and then frozen in skim milk- or in egg yolk-based extenders. Goat oocytes were matured and inseminated by frozen-thawed spermatozoa selected by swim-up. In vitro fertilization was performed in a modified defined medium (mDM), altered experimentally, for 24 h. Embryos were cultured in 50 ?L of c-SOF+NEA for 9 d. The percentages of oocytes exposed to heparin-capacitated spermatozoa, (previously cryopreserved in skim milk- based extender) that cleaved, reached morula, blastocyst and expanded blastocyst stages were 82.8, 57.1, 35.7 and 30.0%, respectively. Without heparin treatment the rates for cleavage, morula, blastocyst and expanded blastocyst stages were 44.3, 31.4, 18.6 and 8.6%, respectively. Therefore, heparin treatment was included in sperm capacitation. Use of spermatozoa with BSA in the IVF medium yielded no cleavage. Although extenders containing 8 to 20% egg yolk enabled good sperm motility after cryopreservation, in vitro fertilizing ability was compromised under our conditions. By contrast, semen commercially processed in season in an egg yolk-based diluent remained effective for IVF. The highest proportion of blastocysts resulted from the use of spermatozoa diluted in a skim milk extender, heparin capacitation, and insemination in medium containing lamb serum. 650 $aAnimal breeding 650 $aBlastocystis 650 $aGoats 650 $aIn vitro fertilization 650 $aReproduction 650 $aCaprino 650 $aCriopreservação 650 $aEspermatozóide 650 $aInseminação artificial 650 $aReprodução animal 650 $aSêmen 653 $aAnimal embryos 653 $aBlastocisto 653 $aExtenders 653 $aOvary 653 $aSperm cryopreservation 653 $aSperm preservation 700 1 $aSIMPLICIO, A. A. 700 1 $aBRACKETT, B. G. 773 $tTheriogenology, New York$gv. 49, n. 7, p. 1265-1274, May, 1998.
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Embrapa Caprinos e Ovinos (CNPC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
04/12/1998 |
Data da última atualização: |
04/12/1998 |
Autoria: |
SANTOS, F. F. dos; CARMO, C. A. S. do; VILELA, N. J. |
Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
Título: |
Colheita, classificacao, embalagem e comercializacao. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
In: SANTOS, F.F. dos; CARMO, C.A.S. do., ed. Mandioquinha-salsa: manejo cultural. Brasilia: EMBRAPA-SPI / EMBRAPA-CNPH, 1998. |
Páginas: |
p.64-79. |
Idioma: |
Português |
Conteúdo: |
Colheita; Classificacao e embalagem; Comercializacao; Custo de producao/retorno; Margens relativas de comercializacao e markup; Perdas pos-colheita; Variacoes estacionais. |
Palavras-Chave: |
Arracacha; Batata-baroa; Classificacao; Harvest; Mandioquinha-salsa; Market; Package; peruvian carrot; Posharvest. |
Thesagro: |
Arracacia Xanthorrhiza; Colheita; Comercialização; Embalagem; Pós-Colheita. |
Thesaurus NAL: |
classification. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01102naa a2200337 a 4500 001 1762014 005 1998-12-04 008 1998 bl uuuu u00u1 u #d 100 1 $aSANTOS, F. F. dos 245 $aColheita, classificacao, embalagem e comercializacao. 260 $c1998 300 $ap.64-79. 520 $aColheita; Classificacao e embalagem; Comercializacao; Custo de producao/retorno; Margens relativas de comercializacao e markup; Perdas pos-colheita; Variacoes estacionais. 650 $aclassification 650 $aArracacia Xanthorrhiza 650 $aColheita 650 $aComercialização 650 $aEmbalagem 650 $aPós-Colheita 653 $aArracacha 653 $aBatata-baroa 653 $aClassificacao 653 $aHarvest 653 $aMandioquinha-salsa 653 $aMarket 653 $aPackage 653 $aperuvian carrot 653 $aPosharvest 700 1 $aCARMO, C. A. S. do 700 1 $aVILELA, N. J. 773 $tIn: SANTOS, F.F. dos; CARMO, C.A.S. do., ed. Mandioquinha-salsa: manejo cultural. Brasilia: EMBRAPA-SPI / EMBRAPA-CNPH, 1998.
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