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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
16/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARAES, A. S.; DORNELES, E. M. S.; ANDRADE, G. I.; LAGE, A. P.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. |
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Afiliação: |
ALESSANDRO DE SA GUIMARAES, CNPGL; ELAINE MARIA SELES DORNELES, UFMG; GIOVANNA IVO ANDRADE, UFMG; ANDREY PEREIRA LAGE, UFMG; GEPOC; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG; GEPOC; AURORA MARIA GUIMARÃES GOUVEIA, UFMG; GEPOC; MARCOS BRYAN HEINEMANN, UFMG; GEPOC. |
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Título: |
Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 153, n. 3/4, p. 299-306, 2011. |
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DOI: |
https://doi.org/10.1016/j.vetmic.2011.06.002 |
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Idioma: |
Inglês |
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Conteúdo: |
Caseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. MenosCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10... Mostrar Tudo |
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Palavras-Chave: |
ERIC-PCR; RAPD. |
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Thesagro: |
Corynebacterium Pseudotuberculosis. |
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Thesaurus Nal: |
caseous lymphadenitis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02507naa a2200265 a 4500
001 1910089
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetmic.2011.06.002$2DOI
100 1 $aGUIMARAES, A. S.
245 $aMolecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR.$h[electronic resource]
260 $c2011
520 $aCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates.
650 $acaseous lymphadenitis
650 $aCorynebacterium Pseudotuberculosis
653 $aERIC-PCR
653 $aRAPD
700 1 $aDORNELES, E. M. S.
700 1 $aANDRADE, G. I.
700 1 $aLAGE, A. P.
700 1 $aMIYOSHI, A.
700 1 $aAZEVEDO, V.
700 1 $aGOUVEIA, A. M. G.
700 1 $aHEINEMANN, M. B.
773 $tVeterinary Microbiology$gv. 153, n. 3/4, p. 299-306, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
03/06/2020 |
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Data da última atualização: |
07/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BEZERRA, C. F.; ROCHA, J. E.; SILVA, M. K. do N.; FREITAS, T. S. de; SOUSA, A. K. de; CARNEIRO, J. N. P.; LEAL, A. L. A. B.; SANTOS, A. T. L. dos; CRUZ, R. P. da; RODRIGUES, A. S.; SALES, D. L.; COUTINHO, H. D. M.; RIBEIRO, P. R. V.; BRITO, E. S. de; COSTA, J. G. M. da; MENEZES, R. R. A. de; ALMEIDA, W. de O.; MORAIS-BRAGA, M. F. B. |
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Afiliação: |
CAMILA FONSECA BEZERRA, Department of Biological Chemistry, Regional University of Cariri, Crato; JANAÍNA ESMERALDO ROCHA, Department of Biological Chemistry, Regional University of Cariri, Crato; MARIA KAROLLYNA DO NASCIMENTO SILVA, Department of Biological Chemistry, Regional University of Cariri, Crato; THIAGO SAMPAIO DE FREITAS, Department of Biological Chemistry, Regional University of Cariri, Crato; AMANDA KARINE DE SOUSA, Department of Biological Chemistry, Regional University of Cariri, Crato; JOARA NALYDA PEREIRA CARNEIRO, Department of Biological Chemistry, Regional University of Cariri, Crato; ANTÔNIO LINKOLN ALVES BORGES LEAL, Department of Biological Chemistry, Regional University of Cariri, Crato; ANTÔNIA THASSYA LUCAS DOS SANTOS, Department of Biological Chemistry, Regional University of Cariri, Crato; RAFAEL PEREIRA DA CRUZ, Department of Biological Chemistry, Regional University of Cariri, Crato; ALLANA SILVA RODRIGUES, Department of Biological Chemistry, Regional University of Cariri, Crato; DEBORA LIMA SALES, Department of Biological Chemistry, Regional University of Cariri, Crato; HENRIQUE DOUGLAS MELO COUTINHO, Department of Biological Chemistry, Regional University of Cariri, Crato; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; EDY SOUSA DE BRITO, CNPAT; JOSE GALBERTO MARTINS DA COSTA, Department of Biological Chemistry, Regional University of Cariri, Crato; RWIN ROSE ALENCAR DE MENEZES, Department of Biological Chemistry, Regional University of Cariri, Crato; WALTÉCIO DE OLIVEIRA ALMEIDA, Department of Biological Chemistry, Regional University of Cariri, Crato; MARIA FLAVIANA BEZERRA MORAIS-BRAGA, Department of Biological Sciences, Regional University of Cariri, Crato. |
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Título: |
UPLC-MS-ESI-QTOF analysis and anti-Candida activity of fractions from Psidium guajava L. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
South African Journal of Botany, 131, pp. 421-427, 2020. |
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DOI: |
https://doi.org/10.1016/j.sajb.2020.03.020 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Antifungal effect; Candida spp; Dimorphism inhibition; Efeito antifúngico; Fluconazol; Inibição do dimorfismo; Psidium guajava L. |
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Thesagro: |
Sinergismo. |
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Thesaurus NAL: |
Fluconazole; Synergism. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01333naa a2200445 a 4500
001 2122950
005 2021-01-07
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.sajb.2020.03.020$2DOI
100 1 $aBEZERRA, C. F.
245 $aUPLC-MS-ESI-QTOF analysis and anti-Candida activity of fractions from Psidium guajava L.$h[electronic resource]
260 $c2020
650 $aFluconazole
650 $aSynergism
650 $aSinergismo
653 $aAntifungal effect
653 $aCandida spp
653 $aDimorphism inhibition
653 $aEfeito antifúngico
653 $aFluconazol
653 $aInibição do dimorfismo
653 $aPsidium guajava L
700 1 $aROCHA, J. E.
700 1 $aSILVA, M. K. do N.
700 1 $aFREITAS, T. S. de
700 1 $aSOUSA, A. K. de
700 1 $aCARNEIRO, J. N. P.
700 1 $aLEAL, A. L. A. B.
700 1 $aSANTOS, A. T. L. dos
700 1 $aCRUZ, R. P. da
700 1 $aRODRIGUES, A. S.
700 1 $aSALES, D. L.
700 1 $aCOUTINHO, H. D. M.
700 1 $aRIBEIRO, P. R. V.
700 1 $aBRITO, E. S. de
700 1 $aCOSTA, J. G. M. da
700 1 $aMENEZES, R. R. A. de
700 1 $aALMEIDA, W. de O.
700 1 $aMORAIS-BRAGA, M. F. B.
773 $tSouth African Journal of Botany, 131, pp. 421-427, 2020.
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Embrapa Agroindústria Tropical (CNPAT) |
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