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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
16/06/2011 |
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Data da última atualização: |
27/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MINGOTI, G. Z.; CASTRO, V. S. D. C.; NICIURA, S. C. M.; BARRETO, L. S. S.; GARCIA, J. M. |
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Afiliação: |
GISELE ZOCCAL MINGOTI, UNESP/ARAÇATUBA; VIVIANE SGGOBI DIAS CAIADO CASTRO, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; LETÍCIA SIQUEIRA SÁ BARETTO, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
The effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In Vitro Cell Dev Biol Anim., v. 47, n. 5-6, p.361-367, jun. 2011. |
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DOI: |
10.1007/s11626-011-9400-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Aiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addition, while defined macromolecules PVA, PVP, and Ficoll were not suitable for embryonic development, the synthetic serum Knockout was able to replace serum and albumin for IVP in bovine at 20% O2. MenosAiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addit... Mostrar Tudo |
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Palavras-Chave: |
Embryo; In vitro maturation; Macromolecule. |
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Thesaurus Nal: |
beef cattle; oxygen. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02490naa a2200241 a 4500
001 1894336
005 2022-07-27
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1007/s11626-011-9400-0$2DOI
100 1 $aMINGOTI, G. Z.
245 $aThe effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development.$h[electronic resource]
260 $c2011
520 $aAiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addition, while defined macromolecules PVA, PVP, and Ficoll were not suitable for embryonic development, the synthetic serum Knockout was able to replace serum and albumin for IVP in bovine at 20% O2.
650 $abeef cattle
650 $aoxygen
653 $aEmbryo
653 $aIn vitro maturation
653 $aMacromolecule
700 1 $aCASTRO, V. S. D. C.
700 1 $aNICIURA, S. C. M.
700 1 $aBARRETO, L. S. S.
700 1 $aGARCIA, J. M.
773 $tIn Vitro Cell Dev Biol Anim.$gv. 47, n. 5-6, p.361-367, jun. 2011.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca |
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
13/12/2022 |
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Data da última atualização: |
15/12/2022 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
SCHWENGBER, J. E.; MAYER, F. |
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Afiliação: |
JOSE ERNANI SCHWENGBER, CPACT; FABIO MAYER. |
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Título: |
Plantio direto de hortaliças tendo a compostagem laminar como estratégia. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: WOLFF, L. F.; EICHOLZ, E. D. (ed.). Alternativas para Diversificação da Agricultura Familiar de Base Ecológica - 2022. Pelotas: Embrapa Clima Temperado, 2022. p. 23-24. (Embrapa Clima Temperado. Documentos, 527). ODS. |
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Idioma: |
Português |
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Conteúdo: |
Práticas de cultivo sem o revolvimento do solo foram estratégias muito utilizadas por povos nativos em várias regiões do mundo, como o sistema de coivara (corte e queima), cultura de ?tapado? (semeadura direta e roçada das plantas espontâneas para recobrimento das sementes) ou mesmo a roçada e o enleiramento das plantas espontâneas e plantio nas linhas. |
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Thesagro: |
Hortaliça; Plantio Direto. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1149601/1/CPACT-Doc-527-pag23.pdf
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Marc: |
LEADER 00997naa a2200157 a 4500
001 2149601
005 2022-12-15
008 2022 bl uuuu u00u1 u #d
100 1 $aSCHWENGBER, J. E.
245 $aPlantio direto de hortaliças tendo a compostagem laminar como estratégia.$h[electronic resource]
260 $c2022
520 $aPráticas de cultivo sem o revolvimento do solo foram estratégias muito utilizadas por povos nativos em várias regiões do mundo, como o sistema de coivara (corte e queima), cultura de ?tapado? (semeadura direta e roçada das plantas espontâneas para recobrimento das sementes) ou mesmo a roçada e o enleiramento das plantas espontâneas e plantio nas linhas.
650 $aHortaliça
650 $aPlantio Direto
700 1 $aMAYER, F.
773 $tIn: WOLFF, L. F.; EICHOLZ, E. D. (ed.). Alternativas para Diversificação da Agricultura Familiar de Base Ecológica - 2022. Pelotas: Embrapa Clima Temperado, 2022. p. 23-24. (Embrapa Clima Temperado. Documentos, 527). ODS.
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