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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/04/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, F. A. C.; PIROVANI, C. P.; MENEZES, S. P.; SANTIAGO, A. S.; CASCARDO, J. M. C.; MICHELI, F. F. L.; GESTEIRA, A. S. |
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Afiliação: |
Fabiana A. C. Silva, UESC; Carlos Priminho Pirovani, UESC; Sara Pereira Menezes, UESC; André da Silva Santiago, UESC; Julio Cezar de Mattos Cascardo, UESC; Fabienne Florence Lucienne Micheli, CIRAD-BIOS; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Action of the pathogenesis-related protein PR10 from Theobroma cacao in triggering response mechanisms of Moniliophtora perniciosa. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
pdf 35180 |
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Conteúdo: |
Cacao (Theobroma cacao L.) is an important commodity worldwide, but its production is limited by destructive diseases such witches? broom, due to the fungus Moniliophthora perniciosa. The high recombination rate and genetic variability of this fungus promoted resistance breakdown of cacao and annihilated the efforts made by the Brazilian government to reduce the disease impact on plantations. According to the literature, pathogenesis related-proteins (PRs) plays an important role in defense/resistance mechanisms of the plant submitted to various biotic and abiotic stresses. A cDNA clone encoding a PR10 (named TcPR10) was obtained from a cacao-M. perniciosa EST library. The corresponding recombinant protein (expressed in Escherichia coli BL21(DE3)) present a strong antifungal activity against M. perniciosa, as previously demonstrated. In this work, we developed a proteomic analysis of the fungus cultivated in the presence of TcPR10, using 2DE-MS/MS. M. perniciosa was grown in CPD 2% agar medium; after 15 days, the fungal hyphae were broken and were grown in presence of 3 µg/mL of TcPR10 for 1h.Then, the total proteins were extracted using the ADP method, followed by a simple cleaning using the method of SDS-dense and phenol. The quantification was made using a 2-D quantification kit. The proteins were extracted in triplicate and separated using a 12% bi-dimensional SDS-PAGE gel. The 2D map analysis showed approximately 300 ?spots? per gel (control and one hour treatment) with differential protein expression pattern. The analysis using a mass spectrometry (naniESI-Q-TOF) was made for the identification of the spots. We identified several proteins involved in fungal metabolism, carbohydrates/proteins metabolism, related proteins to growth and phytotoxics proteins. More spots have been identified to better understand the mechanism of fungi response to protein PR10. MenosCacao (Theobroma cacao L.) is an important commodity worldwide, but its production is limited by destructive diseases such witches? broom, due to the fungus Moniliophthora perniciosa. The high recombination rate and genetic variability of this fungus promoted resistance breakdown of cacao and annihilated the efforts made by the Brazilian government to reduce the disease impact on plantations. According to the literature, pathogenesis related-proteins (PRs) plays an important role in defense/resistance mechanisms of the plant submitted to various biotic and abiotic stresses. A cDNA clone encoding a PR10 (named TcPR10) was obtained from a cacao-M. perniciosa EST library. The corresponding recombinant protein (expressed in Escherichia coli BL21(DE3)) present a strong antifungal activity against M. perniciosa, as previously demonstrated. In this work, we developed a proteomic analysis of the fungus cultivated in the presence of TcPR10, using 2DE-MS/MS. M. perniciosa was grown in CPD 2% agar medium; after 15 days, the fungal hyphae were broken and were grown in presence of 3 µg/mL of TcPR10 for 1h.Then, the total proteins were extracted using the ADP method, followed by a simple cleaning using the method of SDS-dense and phenol. The quantification was made using a 2-D quantification kit. The proteins were extracted in triplicate and separated using a 12% bi-dimensional SDS-PAGE gel. The 2D map analysis showed approximately 300 ?spots? per gel (control and one hour treatment) with... Mostrar Tudo |
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Palavras-Chave: |
Bi-dimensional gel; Fungal disease; Pathogenesis related-protein PR10; TcPR10 protein. |
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Thesaurus Nal: |
mass spectrometry. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02785nam a2200253 a 4500
001 1887016
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aSILVA, F. A. C.
245 $aAction of the pathogenesis-related protein PR10 from Theobroma cacao in triggering response mechanisms of Moniliophtora perniciosa.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 35180
520 $aCacao (Theobroma cacao L.) is an important commodity worldwide, but its production is limited by destructive diseases such witches? broom, due to the fungus Moniliophthora perniciosa. The high recombination rate and genetic variability of this fungus promoted resistance breakdown of cacao and annihilated the efforts made by the Brazilian government to reduce the disease impact on plantations. According to the literature, pathogenesis related-proteins (PRs) plays an important role in defense/resistance mechanisms of the plant submitted to various biotic and abiotic stresses. A cDNA clone encoding a PR10 (named TcPR10) was obtained from a cacao-M. perniciosa EST library. The corresponding recombinant protein (expressed in Escherichia coli BL21(DE3)) present a strong antifungal activity against M. perniciosa, as previously demonstrated. In this work, we developed a proteomic analysis of the fungus cultivated in the presence of TcPR10, using 2DE-MS/MS. M. perniciosa was grown in CPD 2% agar medium; after 15 days, the fungal hyphae were broken and were grown in presence of 3 µg/mL of TcPR10 for 1h.Then, the total proteins were extracted using the ADP method, followed by a simple cleaning using the method of SDS-dense and phenol. The quantification was made using a 2-D quantification kit. The proteins were extracted in triplicate and separated using a 12% bi-dimensional SDS-PAGE gel. The 2D map analysis showed approximately 300 ?spots? per gel (control and one hour treatment) with differential protein expression pattern. The analysis using a mass spectrometry (naniESI-Q-TOF) was made for the identification of the spots. We identified several proteins involved in fungal metabolism, carbohydrates/proteins metabolism, related proteins to growth and phytotoxics proteins. More spots have been identified to better understand the mechanism of fungi response to protein PR10.
650 $amass spectrometry
653 $aBi-dimensional gel
653 $aFungal disease
653 $aPathogenesis related-protein PR10
653 $aTcPR10 protein
700 1 $aPIROVANI, C. P.
700 1 $aMENEZES, S. P.
700 1 $aSANTIAGO, A. S.
700 1 $aCASCARDO, J. M. C.
700 1 $aMICHELI, F. F. L.
700 1 $aGESTEIRA, A. S.
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