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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
18/10/2010 |
Data da última atualização: |
19/10/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ANDRADE, E. C.; SANTOS, K. C.; MEISSNER, P. E. |
Afiliação: |
EDUARDO CHUMBINHO DE ANDRADE, CNPMF; Keila Cidreira Santos, FAMAM; PAULO ERNESTO MEISSNER FILHO, CNPMF. |
Título: |
Development of degenerated primers to detect different viruses associated with mealybug wilt of pineapple. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Pineapple News, n. 17, p. 8, Aug. 2010. Addendum to Newsletter Pineapple Working Group of the I.S.H.S. |
Idioma: |
Inglês |
Notas: |
Disponível em: < http://www.ishs-horticulture.org/workinggroups/pineapple/PineNews17_addendum.pdf> Acesso em: 16 fev. 2011.
Edição de Abstracts of the 7th International Pineapple Symposium, Johor Baru, Malaysia, 2010. |
Conteúdo: |
Pineapple (Ananas comosus var. comosus) is propagated vegetatively which facilitates the production of planting material but leads to dissemination of viruses. Mealybug wilt of pineapple (MWP) is a devastating disease of pineapple worldwide. The disease is characterized by severe leaf tip dieback, wilting of the leaves and can lead the plant to die. The MWP is associated with three Ampelovirus species, Pineapple mealybug wilt associated virus-1 (PMWaV-1), PMWaV-2, and PMWaV-3. Besides to the direct damage on plant production, there is great difficulty to select plantlets for planting because some infected plants exhibits no symptoms. The virus can be detected by RT PCR using specific primers; however for phytosanitary purposed, it's interesting to screen plant material for all viruses simultaneously in order to save time and cost. With this objective, degenerate primers were designed and tested to detect PMWaV-1, -2, -3 in pineapple hybrids of Embrapa breeding program. Using the degenerate primers in a RT PCR, it was possible to amplify a fragment of the expected size only in infected samples. Also, the reverse degenerated primer was combined with each of the forward specific primers in order to identify the PMWaV species present in infected plant. These two approaches led to detection of PMWaVs in 20/27 asymptomatic hybrids, with higher prevalence of PMWaV-1 (12 plants), PMWaV-2 (10 plants) and PMWaV-3 (7 plants). A total of 12 plants present mixed infections. The results show that degenerated primers provided a reliable alternative to plant material certification with about 30-40% on cost reduction and short period of indexing. MenosPineapple (Ananas comosus var. comosus) is propagated vegetatively which facilitates the production of planting material but leads to dissemination of viruses. Mealybug wilt of pineapple (MWP) is a devastating disease of pineapple worldwide. The disease is characterized by severe leaf tip dieback, wilting of the leaves and can lead the plant to die. The MWP is associated with three Ampelovirus species, Pineapple mealybug wilt associated virus-1 (PMWaV-1), PMWaV-2, and PMWaV-3. Besides to the direct damage on plant production, there is great difficulty to select plantlets for planting because some infected plants exhibits no symptoms. The virus can be detected by RT PCR using specific primers; however for phytosanitary purposed, it's interesting to screen plant material for all viruses simultaneously in order to save time and cost. With this objective, degenerate primers were designed and tested to detect PMWaV-1, -2, -3 in pineapple hybrids of Embrapa breeding program. Using the degenerate primers in a RT PCR, it was possible to amplify a fragment of the expected size only in infected samples. Also, the reverse degenerated primer was combined with each of the forward specific primers in order to identify the PMWaV species present in infected plant. These two approaches led to detection of PMWaVs in 20/27 asymptomatic hybrids, with higher prevalence of PMWaV-1 (12 plants), PMWaV-2 (10 plants) and PMWaV-3 (7 plants). A total of 12 plants present mixed infections. The results s... Mostrar Tudo |
Palavras-Chave: |
Pineapple; Plant disease. |
Thesagro: |
Abacaxi; Doença de Planta. |
Thesaurus Nal: |
Ananas comosus var. comosus. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02561nam a2200205 a 4500 001 1864522 005 2023-10-19 008 2010 bl uuuu u00u1 u #d 100 1 $aANDRADE, E. C. 245 $aDevelopment of degenerated primers to detect different viruses associated with mealybug wilt of pineapple.$h[electronic resource] 260 $aPineapple News, n. 17, p. 8, Aug. 2010. Addendum to Newsletter Pineapple Working Group of the I.S.H.S.$c2010 500 $aDisponível em: < http://www.ishs-horticulture.org/workinggroups/pineapple/PineNews17_addendum.pdf> Acesso em: 16 fev. 2011. Edição de Abstracts of the 7th International Pineapple Symposium, Johor Baru, Malaysia, 2010. 520 $aPineapple (Ananas comosus var. comosus) is propagated vegetatively which facilitates the production of planting material but leads to dissemination of viruses. Mealybug wilt of pineapple (MWP) is a devastating disease of pineapple worldwide. The disease is characterized by severe leaf tip dieback, wilting of the leaves and can lead the plant to die. The MWP is associated with three Ampelovirus species, Pineapple mealybug wilt associated virus-1 (PMWaV-1), PMWaV-2, and PMWaV-3. Besides to the direct damage on plant production, there is great difficulty to select plantlets for planting because some infected plants exhibits no symptoms. The virus can be detected by RT PCR using specific primers; however for phytosanitary purposed, it's interesting to screen plant material for all viruses simultaneously in order to save time and cost. With this objective, degenerate primers were designed and tested to detect PMWaV-1, -2, -3 in pineapple hybrids of Embrapa breeding program. Using the degenerate primers in a RT PCR, it was possible to amplify a fragment of the expected size only in infected samples. Also, the reverse degenerated primer was combined with each of the forward specific primers in order to identify the PMWaV species present in infected plant. These two approaches led to detection of PMWaVs in 20/27 asymptomatic hybrids, with higher prevalence of PMWaV-1 (12 plants), PMWaV-2 (10 plants) and PMWaV-3 (7 plants). A total of 12 plants present mixed infections. The results show that degenerated primers provided a reliable alternative to plant material certification with about 30-40% on cost reduction and short period of indexing. 650 $aAnanas comosus var. comosus 650 $aAbacaxi 650 $aDoença de Planta 653 $aPineapple 653 $aPlant disease 700 1 $aSANTOS, K. C. 700 1 $aMEISSNER, P. E.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
09/04/2010 |
Data da última atualização: |
06/04/2011 |
Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
Autoria: |
CATELLA, A. C.; SILVA, J. M. V. da; JESUS, V. M. F. de. |
Afiliação: |
AGOSTINHO CARLOS CATELLA, CPAP; JOSINEIDY MIRIÃ VIGABRIEL DA SILVA, UFMS; VANDER MELQUÍADES FABRÍCIO DE JESUS, IMASUL. |
Título: |
Comércio de iscas vivas no Pantanal de Mato Grosso do Sul, SCPESCA/MS, 2005. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Corumbá: Embrapa Pantanal, 2009. |
Páginas: |
42 p. |
Série: |
(Embrapa Pantanal. Boletim de Pesquisa e Desenvolvimento, 90). |
Idioma: |
Português |
Conteúdo: |
O monitoramento pelo SCPESCA/MS constitui um exemplo gratificante de parceria entre instituições que atuam junto à pesca no Pantanal. O Sistema, que não seria possível sem esse esforço conjunto, gera resultados como a descrição anual da pesca e vem formando um grande banco de dados sobre a atividade, constituindo uma fonte importante de informações e conhecimentos para subsidiar a gestão dos recursos pesqueiros da região. Este estudo aborda o comércio de iscas vivas registrado pelo Sistema de Controle da Pesca de Mato Grosso do Sul (SCPESCA/MS) em 2005. Trata-se do comércio no atacado, realizado por intermediários entre municípios e entre Estados, pois a venda local (no atacado ou varejo) é feita diretamente entre as partes e não é contabilizada pelo Sistema. |
Palavras-Chave: |
áreas úmidas; Bacia do Alto Paraguai; pesca de águas interiores; pesca de iscas; pesca esportiva. |
Thesagro: |
Pesca Artesanal. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAP-2010/57289/1/BP90.pdf
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Marc: |
LEADER 01480nam a2200229 a 4500 001 1806080 005 2011-04-06 008 2009 bl uuuu u0uu1 u #d 100 1 $aCATELLA, A. C. 245 $aComércio de iscas vivas no Pantanal de Mato Grosso do Sul, SCPESCA/MS, 2005. 260 $aCorumbá: Embrapa Pantanal$c2009 300 $a42 p. 490 $a(Embrapa Pantanal. Boletim de Pesquisa e Desenvolvimento, 90). 520 $aO monitoramento pelo SCPESCA/MS constitui um exemplo gratificante de parceria entre instituições que atuam junto à pesca no Pantanal. O Sistema, que não seria possível sem esse esforço conjunto, gera resultados como a descrição anual da pesca e vem formando um grande banco de dados sobre a atividade, constituindo uma fonte importante de informações e conhecimentos para subsidiar a gestão dos recursos pesqueiros da região. Este estudo aborda o comércio de iscas vivas registrado pelo Sistema de Controle da Pesca de Mato Grosso do Sul (SCPESCA/MS) em 2005. Trata-se do comércio no atacado, realizado por intermediários entre municípios e entre Estados, pois a venda local (no atacado ou varejo) é feita diretamente entre as partes e não é contabilizada pelo Sistema. 650 $aPesca Artesanal 653 $aáreas úmidas 653 $aBacia do Alto Paraguai 653 $apesca de águas interiores 653 $apesca de iscas 653 $apesca esportiva 700 1 $aSILVA, J. M. V. da 700 1 $aJESUS, V. M. F. de
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