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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
01/02/2000 |
Data da última atualização: |
10/08/2023 |
Autoria: |
NIKOLARAKIS, K. E.; PFEIFFER, D. G.; ALMEIDA, O. F. X.; HERZ, A. |
Título: |
Opioid modulation of LHRH release in vitro depends upon levels of testosterone in vivo. |
Ano de publicação: |
1986 |
Fonte/Imprenta: |
Neuroendocrinology, v. 44, n. 3, p. 314-319, 1986. |
DOI: |
10.1159/000124662. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The in vitro release of LHRH from hypothalami of adult male rats (intact, 5-day castrates, 5-day castrates replaced with various doses of testosterone) was measured under basal conditions and after the addition of KCl, the opiate antagonist naloxone or the opiate agonist DAGO to the perifusion medium. Hypothalami from all treatment groups responded to 56 mM KCl with an increased output of LHRH. LHRH release was also induced by naloxone (10(-6)M), but only from tissues derived from intacts and castrates given physiological doses of testosterone. The opiate agonist DAGO (10(-6)M) did not alter the basal release of LHRH; it, however, caused a significant decrease in the K+-induced release of LHRH from hypothalami derived from intact rats and rats replaced with physiological levels of testosterone but not from those derived from castrate rats or castrate rats replaced with small amounts of testosterone. The specificity of this latter response was shown by its reversibility with naloxone. The lack of DAGO effects upon tissues from rats with low levels of steroid implied steroid dependency of the response to opioidergic influences and indeed, the response to DAGO was restored when testosterone was replaced at physiological doses. Measurement of hypothalamic LHRH content showed no significant differences between tissues obtained from intact, castrate and testosterone-replaced castrate rats. These in vitro data support the view that the inhibitory influence of opioids upon LHRH release depends on the presence of gonadal steroids in vivo. MenosAbstract: The in vitro release of LHRH from hypothalami of adult male rats (intact, 5-day castrates, 5-day castrates replaced with various doses of testosterone) was measured under basal conditions and after the addition of KCl, the opiate antagonist naloxone or the opiate agonist DAGO to the perifusion medium. Hypothalami from all treatment groups responded to 56 mM KCl with an increased output of LHRH. LHRH release was also induced by naloxone (10(-6)M), but only from tissues derived from intacts and castrates given physiological doses of testosterone. The opiate agonist DAGO (10(-6)M) did not alter the basal release of LHRH; it, however, caused a significant decrease in the K+-induced release of LHRH from hypothalami derived from intact rats and rats replaced with physiological levels of testosterone but not from those derived from castrate rats or castrate rats replaced with small amounts of testosterone. The specificity of this latter response was shown by its reversibility with naloxone. The lack of DAGO effects upon tissues from rats with low levels of steroid implied steroid dependency of the response to opioidergic influences and indeed, the response to DAGO was restored when testosterone was replaced at physiological doses. Measurement of hypothalamic LHRH content showed no significant differences between tissues obtained from intact, castrate and testosterone-replaced castrate rats. These in vitro data support the view that the inhibitory influence of opioids upon... Mostrar Tudo |
Palavras-Chave: |
Enkephalins; LHRH; Opioide; Orchiectomy; Sex steroids. |
Thesagro: |
Rato; Testosterona. |
Thesaurus Nal: |
Endorphins; Gonadotropin-releasing hormone; Hypothalamus; Luteinizing hormone; Metabolism; Pharmacology; Physiology; Rats; Synaptic transmission; Testosterone. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02575naa a2200373 a 4500 001 1524096 005 2023-08-10 008 1986 bl uuuu u00u1 u #d 024 7 $a10.1159/000124662.$2DOI 100 1 $aNIKOLARAKIS, K. E. 245 $aOpioid modulation of LHRH release in vitro depends upon levels of testosterone in vivo.$h[electronic resource] 260 $c1986 520 $aAbstract: The in vitro release of LHRH from hypothalami of adult male rats (intact, 5-day castrates, 5-day castrates replaced with various doses of testosterone) was measured under basal conditions and after the addition of KCl, the opiate antagonist naloxone or the opiate agonist DAGO to the perifusion medium. Hypothalami from all treatment groups responded to 56 mM KCl with an increased output of LHRH. LHRH release was also induced by naloxone (10(-6)M), but only from tissues derived from intacts and castrates given physiological doses of testosterone. The opiate agonist DAGO (10(-6)M) did not alter the basal release of LHRH; it, however, caused a significant decrease in the K+-induced release of LHRH from hypothalami derived from intact rats and rats replaced with physiological levels of testosterone but not from those derived from castrate rats or castrate rats replaced with small amounts of testosterone. The specificity of this latter response was shown by its reversibility with naloxone. The lack of DAGO effects upon tissues from rats with low levels of steroid implied steroid dependency of the response to opioidergic influences and indeed, the response to DAGO was restored when testosterone was replaced at physiological doses. Measurement of hypothalamic LHRH content showed no significant differences between tissues obtained from intact, castrate and testosterone-replaced castrate rats. These in vitro data support the view that the inhibitory influence of opioids upon LHRH release depends on the presence of gonadal steroids in vivo. 650 $aEndorphins 650 $aGonadotropin-releasing hormone 650 $aHypothalamus 650 $aLuteinizing hormone 650 $aMetabolism 650 $aPharmacology 650 $aPhysiology 650 $aRats 650 $aSynaptic transmission 650 $aTestosterone 650 $aRato 650 $aTestosterona 653 $aEnkephalins 653 $aLHRH 653 $aOpioide 653 $aOrchiectomy 653 $aSex steroids 700 1 $aPFEIFFER, D. G. 700 1 $aALMEIDA, O. F. X. 700 1 $aHERZ, A. 773 $tNeuroendocrinology$gv. 44, n. 3, p. 314-319, 1986.
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Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
30/09/2008 |
Data da última atualização: |
23/08/2022 |
Tipo da produção científica: |
Artigo de Divulgação na Mídia |
Autoria: |
LIMA NETO, F. P.; SANTOS, C. A. F.; LIMA FILHO, J. M. P.; SANTOS, I. C. N. |
Afiliação: |
FRANCISCO PINHEIRO LIMA NETO, CPATSA; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; José Moacir Pinheiro Lima Filho, CPATSA; Ierla Carla N. Santos, FACEPE. |
Título: |
Progressos do programa de melhoramento genético da mangueira na Embrapa Semi-Árido. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Jornal da Fruta, Lages, v. 16, n. 202, p. 25, jul. 2008. |
Idioma: |
Português |
Conteúdo: |
O objetivo da obtenção de híbridos entre as diferentes variedades de mangueiras. |
Palavras-Chave: |
Melhoramento genético. |
Thesagro: |
Manga. |
Thesaurus NAL: |
Mangoes. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186862/1/Jornal-da-Fruta-v.16-n.202-p.25-2008.pdf
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Marc: |
LEADER 00624nam a2200181 a 4500 001 1161763 005 2022-08-23 008 2008 bl uuuu u00u1 u #d 100 1 $aLIMA NETO, F. P. 245 $aProgressos do programa de melhoramento genético da mangueira na Embrapa Semi-Árido. 260 $aJornal da Fruta, Lages, v. 16, n. 202, p. 25, jul. 2008.$c2008 520 $aO objetivo da obtenção de híbridos entre as diferentes variedades de mangueiras. 650 $aMangoes 650 $aManga 653 $aMelhoramento genético 700 1 $aSANTOS, C. A. F. 700 1 $aLIMA FILHO, J. M. P. 700 1 $aSANTOS, I. C. N.
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