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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/11/2005 |
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Data da última atualização: |
24/05/2022 |
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Autoria: |
MENDES, R. A.; ALLEM, A. C.; ABIORANA, A. F. |
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Título: |
Conservação de recursos genéticos de Manihot mediante a utilização de coleção dinâmica. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
CONGRESSO BRASILEIRO DE MANDIOCA, 11., 2005, Campo Grande, MS. Ciência e tecnologia para a raiz do Brasil: anais. Campo Grande: Governo do Estado de Mato Grosso do Sul; Dourados: Embrapa Agropecuária Oeste, 2005. |
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Idioma: |
Português |
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Palavras-Chave: |
Conservação de recursos; Recurso genético vegetal. |
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Thesagro: |
Mandioca. |
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Thesaurus Nal: |
Manihot. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00711nam a2200169 a 4500
001 1186318
005 2022-05-24
008 2005 bl uuuu u00u1 u #d
100 1 $aMENDES, R. A.
245 $aConservação de recursos genéticos de Manihot mediante a utilização de coleção dinâmica.$h[electronic resource]
260 $aCONGRESSO BRASILEIRO DE MANDIOCA, 11., 2005, Campo Grande, MS. Ciência e tecnologia para a raiz do Brasil: anais. Campo Grande: Governo do Estado de Mato Grosso do Sul; Dourados: Embrapa Agropecuária Oeste$c2005
650 $aManihot
650 $aMandioca
653 $aConservação de recursos
653 $aRecurso genético vegetal
700 1 $aALLEM, A. C.
700 1 $aABIORANA, A. F.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Amazônia Ocidental. |
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Data corrente: |
06/03/2023 |
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Data da última atualização: |
01/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 3 |
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Autoria: |
CASTRO, G. S.; SOUZA, T. F.; SILVA, G. F. da; PEDROSO, R. C. N.; MENEZES, K. S.; SOARES, M. A.; DIAS, G. M.; SANTOS, A. O.; YAMAGISHI, M. E. B.; FARIA, J. V.; JANUÁRIO, A. H.; KOOLEN, H. H. F. |
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Afiliação: |
GLEUCINEI S. CASTRO, UNIVERSIDADE DO ESTADO DO AMAZONAS; THIAGO F. SOUSA, UNIVERSIDADE FEDERAL DO AMAZONAS; GILVAN FERREIRA DA SILVA, CPAA; RITA C. N. PEDROSO, UNIVERSIDADE DE FRANCA; KELLY S. MENEZES, UNIVERSIDADE DO ESTADO DO AMAZONAS; MARCOS A. SOARES, UNIVERSIDADE FEDERAL DE MATO GROSSO; GUSTAVO M. DIAS, UNIVERSIDADE FEDERAL DO ABC; ALINE O. SANTOS, UNIVERSIDADE DO ESTADO DO AMAZONAS; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; JÉSSICA V. FARIA, UNIVERSIDADE DO ESTADO DO AMAZONAS; ANA H. JANUÁRIO, UNIVERSIDADE DE FRANCA; HECTOR H. F. KOOLEN, UNIVERSIDADE DO ESTADO DO AMAZONAS. |
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Título: |
Characterization of peptaibols produced by a marine strain of the fungus Trichoderma endophyticum via Mass Spectrometry, genome mining and phylogeny-based prediction. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Metabolites, v. 13, n. 2, 221, Feb. 2023. |
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DOI: |
https://doi.org/10.3390/metabo13020221 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract. Trichoderma is recognized as a prolific producer of nonribosomal peptides (NRPs) known as peptaibols, which have remarkable biological properties, such as antimicrobial and anticancer activities, as well as the ability to promote systemic resistance in plants against pathogens. In this study, the sequencing of 11-, 14- and 15-res peptaibols produced by a marine strain of Trichoderma isolated from the ascidian Botrylloides giganteus was performed via liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). Identification, based on multilocus phylogeny, revealed that our isolate belongs to the species T. endophyticum, which has never been reported in marine environments. Through genome sequencing and genome mining, 53 biosynthetic gene clusters (BGCs) were identified as being related to bioactive natural products, including two NRP-synthetases: one responsible for the biosynthesis of 11- and 14-res peptaibols, and another for the biosynthesis of 15-res. Substrate prediction, based on phylogeny of the adenylation domains in combination with molecular networking, permitted extensive annotation of the mass spectra related to two new series of 15-res peptaibols, which are referred to herein as "endophytins". The analyses of synteny revealed that the origin of the 15-module peptaibol synthetase is related to 18, 19 and 20-module peptaibol synthetases, and suggests that the loss of modules may be a mechanism used by Trichoderma species for peptaibol diversification. This study demonstrates the importance of combining genome mining techniques, mass spectrometry analysis and molecular networks for the discovery of new natural products. MenosAbstract. Trichoderma is recognized as a prolific producer of nonribosomal peptides (NRPs) known as peptaibols, which have remarkable biological properties, such as antimicrobial and anticancer activities, as well as the ability to promote systemic resistance in plants against pathogens. In this study, the sequencing of 11-, 14- and 15-res peptaibols produced by a marine strain of Trichoderma isolated from the ascidian Botrylloides giganteus was performed via liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). Identification, based on multilocus phylogeny, revealed that our isolate belongs to the species T. endophyticum, which has never been reported in marine environments. Through genome sequencing and genome mining, 53 biosynthetic gene clusters (BGCs) were identified as being related to bioactive natural products, including two NRP-synthetases: one responsible for the biosynthesis of 11- and 14-res peptaibols, and another for the biosynthesis of 15-res. Substrate prediction, based on phylogeny of the adenylation domains in combination with molecular networking, permitted extensive annotation of the mass spectra related to two new series of 15-res peptaibols, which are referred to herein as "endophytins". The analyses of synteny revealed that the origin of the 15-module peptaibol synthetase is related to 18, 19 and 20-module peptaibol synthetases, and suggests that the loss of modules may be a mechanism used by Trichoderma species for pept... Mostrar Tudo |
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Palavras-Chave: |
De novo sequencing; Fungo marinho; Marine fungus; Molecular networking; Nonribosomal peptides; Peptídeos não ribossomais; Rede molecular; Synteny analysis; Tricoderma. |
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Thesagro: |
Peptídeo. |
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Thesaurus NAL: |
Hypocreaceae. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1152102/1/metabolites-13-00221-v2.pdf
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Marc: |
LEADER 02911naa a2200397 a 4500
001 2152102
005 2023-09-01
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/metabo13020221$2DOI
100 1 $aCASTRO, G. S.
245 $aCharacterization of peptaibols produced by a marine strain of the fungus Trichoderma endophyticum via Mass Spectrometry, genome mining and phylogeny-based prediction.$h[electronic resource]
260 $c2023
520 $aAbstract. Trichoderma is recognized as a prolific producer of nonribosomal peptides (NRPs) known as peptaibols, which have remarkable biological properties, such as antimicrobial and anticancer activities, as well as the ability to promote systemic resistance in plants against pathogens. In this study, the sequencing of 11-, 14- and 15-res peptaibols produced by a marine strain of Trichoderma isolated from the ascidian Botrylloides giganteus was performed via liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). Identification, based on multilocus phylogeny, revealed that our isolate belongs to the species T. endophyticum, which has never been reported in marine environments. Through genome sequencing and genome mining, 53 biosynthetic gene clusters (BGCs) were identified as being related to bioactive natural products, including two NRP-synthetases: one responsible for the biosynthesis of 11- and 14-res peptaibols, and another for the biosynthesis of 15-res. Substrate prediction, based on phylogeny of the adenylation domains in combination with molecular networking, permitted extensive annotation of the mass spectra related to two new series of 15-res peptaibols, which are referred to herein as "endophytins". The analyses of synteny revealed that the origin of the 15-module peptaibol synthetase is related to 18, 19 and 20-module peptaibol synthetases, and suggests that the loss of modules may be a mechanism used by Trichoderma species for peptaibol diversification. This study demonstrates the importance of combining genome mining techniques, mass spectrometry analysis and molecular networks for the discovery of new natural products.
650 $aHypocreaceae
650 $aPeptídeo
653 $aDe novo sequencing
653 $aFungo marinho
653 $aMarine fungus
653 $aMolecular networking
653 $aNonribosomal peptides
653 $aPeptídeos não ribossomais
653 $aRede molecular
653 $aSynteny analysis
653 $aTricoderma
700 1 $aSOUZA, T. F.
700 1 $aSILVA, G. F. da
700 1 $aPEDROSO, R. C. N.
700 1 $aMENEZES, K. S.
700 1 $aSOARES, M. A.
700 1 $aDIAS, G. M.
700 1 $aSANTOS, A. O.
700 1 $aYAMAGISHI, M. E. B.
700 1 $aFARIA, J. V.
700 1 $aJANUÁRIO, A. H.
700 1 $aKOOLEN, H. H. F.
773 $tMetabolites$gv. 13, n. 2, 221, Feb. 2023.
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Embrapa Amazônia Ocidental (CPAA) |
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