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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
15/05/1995 |
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Data da última atualização: |
03/07/2014 |
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Autoria: |
MÔRO, J. R. |
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Afiliação: |
JOSÉ ROBERTO MÔRO. |
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Título: |
Produção de palmito de pupunha no Nordeste do Brasil: variabilidade genética e desenvolvimento de cultivares. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
In: QUEIROZ, M. A. de; GOEDERT, C. O.; RAMOS, S. R. R. (Ed.). Recursos genéticos e melhoramento de plantas para o Nordeste brasileiro. Petrolina: Embrapa Semi-Árido; Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 1999. |
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Idioma: |
Português |
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Palavras-Chave: |
Melhoramento genético; Palmetto. |
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Thesagro: |
Palmito; Pupunha; Recurso Genético; Recurso Vegetal. |
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Thesaurus Nal: |
Genetic improvement. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 00788naa a2200193 a 4500
001 1144143
005 2014-07-03
008 1999 bl uuuu u00u1 u #d
100 1 $aMÔRO, J. R.
245 $aProdução de palmito de pupunha no Nordeste do Brasil$bvariabilidade genética e desenvolvimento de cultivares.$h[electronic resource]
260 $c1999
650 $aGenetic improvement
650 $aPalmito
650 $aPupunha
650 $aRecurso Genético
650 $aRecurso Vegetal
653 $aMelhoramento genético
653 $aPalmetto
773 $tIn: QUEIROZ, M. A. de; GOEDERT, C. O.; RAMOS, S. R. R. (Ed.). Recursos genéticos e melhoramento de plantas para o Nordeste brasileiro. Petrolina: Embrapa Semi-Árido; Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 1999.
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Embrapa Semiárido (CPATSA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
09/12/2019 |
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Data da última atualização: |
09/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SANTOS, J. M. L. dos; VASCONCELOS, J. F.; FROTA, G. A.; FREITAS, E. P. de; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L.; MONTEIRO, J. P. |
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Afiliação: |
JESSICA MARIA LEITE DOS SANTOS, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; JANAÉLIA FERREIRA VASCONCELOS, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; GRACIELLE ARAÚJO FROTA, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; EDILSON PEREIRA DE FREITAS, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; MARCEL TEIXEIRA, CNPC; LUIZ DA SILVA VIEIRA, CNPC; CLAUDIA MARIA LEAL BEVILAQUA, Pós-graduação - Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brazil; JOMAR PATRICIO MONTEIRO, CNPC. |
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Título: |
Quantitative molecular diagnosis of levamisole resistance in populations of Haemonchus contortus. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Experimental Parasitology, v. 205, article 107734, Aug. 2019. |
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DOI: |
http://dx.doi.org/10.1016/j.exppara.2019.107734 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Parasitism by Haemonchus contortus is one of the main limiting factors in small ruminant production around the globe. Although several studies suggest the use of integrated management practices, these parasites have been controlled essentially with synthetic anthelmintic drugs. The resistance mechanism against the imidazothiazole derivative levamisole in Haemonchus contortus has not been fully described. Recently, resistance was associated with a 63bp deletion in the Hco-acr-8b gene that encodes a subunit for a nicotinic acetylcholine receptor. This study aimed to standardize a real time PCR (qPCR) protocol for levamisole resistance diagnosis in H. contortus populations based on this polymorphism and use it to characterize 23 field H. contortus populations obtained from different localities of Ceará State, Northeast Brazil. In addition, two populations of H. contortus were used as a standard of susceptibility and resistance, Inbred Strain Edinburgh (ISE) and Kokstad, respectively. Larval development tests (LDT) were performed on five field isolates and both EC50 and EC95 were estimated. LDT EC95 values provided a wider interval between susceptible and resistant populations than EC50 values (EC95?=?1.96-57.93??M; EC50?=?0.05-0.39??M), and were found to be more appropriate for differentiating them. Real time PCR results showed resistance allele frequencies ranged from 20.9 to 76.7%. Our results suggest that levamisole resistance may be present in field populations but it is not as widespread as benzimidazole resistance. This methodology may be useful to monitor levamisole resistance in field populations of H. contortus. MenosAbstract: Parasitism by Haemonchus contortus is one of the main limiting factors in small ruminant production around the globe. Although several studies suggest the use of integrated management practices, these parasites have been controlled essentially with synthetic anthelmintic drugs. The resistance mechanism against the imidazothiazole derivative levamisole in Haemonchus contortus has not been fully described. Recently, resistance was associated with a 63bp deletion in the Hco-acr-8b gene that encodes a subunit for a nicotinic acetylcholine receptor. This study aimed to standardize a real time PCR (qPCR) protocol for levamisole resistance diagnosis in H. contortus populations based on this polymorphism and use it to characterize 23 field H. contortus populations obtained from different localities of Ceará State, Northeast Brazil. In addition, two populations of H. contortus were used as a standard of susceptibility and resistance, Inbred Strain Edinburgh (ISE) and Kokstad, respectively. Larval development tests (LDT) were performed on five field isolates and both EC50 and EC95 were estimated. LDT EC95 values provided a wider interval between susceptible and resistant populations than EC50 values (EC95?=?1.96-57.93??M; EC50?=?0.05-0.39??M), and were found to be more appropriate for differentiating them. Real time PCR results showed resistance allele frequencies ranged from 20.9 to 76.7%. Our results suggest that levamisole resistance may be present in field populations bu... Mostrar Tudo |
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Palavras-Chave: |
Anthelmintic resistance; Antinematodal Agents; Drug effects; Haemonchiasis; Nematode parasite; Real-time PCR. |
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Thesaurus NAL: |
Benzimidazoles; Drug resistance; Gene frequency; Genetics; Helminths; Levamisole; Nematode larvae; Parasitology; Pharmacology; Sequence alignment; Sheep diseases; Tetramisole. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02921naa a2200433 a 4500
001 2116311
005 2019-12-09
008 2019 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.exppara.2019.107734$2DOI
100 1 $aSANTOS, J. M. L. dos
245 $aQuantitative molecular diagnosis of levamisole resistance in populations of Haemonchus contortus.$h[electronic resource]
260 $c2019
520 $aAbstract: Parasitism by Haemonchus contortus is one of the main limiting factors in small ruminant production around the globe. Although several studies suggest the use of integrated management practices, these parasites have been controlled essentially with synthetic anthelmintic drugs. The resistance mechanism against the imidazothiazole derivative levamisole in Haemonchus contortus has not been fully described. Recently, resistance was associated with a 63bp deletion in the Hco-acr-8b gene that encodes a subunit for a nicotinic acetylcholine receptor. This study aimed to standardize a real time PCR (qPCR) protocol for levamisole resistance diagnosis in H. contortus populations based on this polymorphism and use it to characterize 23 field H. contortus populations obtained from different localities of Ceará State, Northeast Brazil. In addition, two populations of H. contortus were used as a standard of susceptibility and resistance, Inbred Strain Edinburgh (ISE) and Kokstad, respectively. Larval development tests (LDT) were performed on five field isolates and both EC50 and EC95 were estimated. LDT EC95 values provided a wider interval between susceptible and resistant populations than EC50 values (EC95?=?1.96-57.93??M; EC50?=?0.05-0.39??M), and were found to be more appropriate for differentiating them. Real time PCR results showed resistance allele frequencies ranged from 20.9 to 76.7%. Our results suggest that levamisole resistance may be present in field populations but it is not as widespread as benzimidazole resistance. This methodology may be useful to monitor levamisole resistance in field populations of H. contortus.
650 $aBenzimidazoles
650 $aDrug resistance
650 $aGene frequency
650 $aGenetics
650 $aHelminths
650 $aLevamisole
650 $aNematode larvae
650 $aParasitology
650 $aPharmacology
650 $aSequence alignment
650 $aSheep diseases
650 $aTetramisole
653 $aAnthelmintic resistance
653 $aAntinematodal Agents
653 $aDrug effects
653 $aHaemonchiasis
653 $aNematode parasite
653 $aReal-time PCR
700 1 $aVASCONCELOS, J. F.
700 1 $aFROTA, G. A.
700 1 $aFREITAS, E. P. de
700 1 $aTEIXEIRA, M.
700 1 $aVIEIRA, L. da S.
700 1 $aBEVILAQUA, C. M. L.
700 1 $aMONTEIRO, J. P.
773 $tExperimental Parasitology$gv. 205, article 107734, Aug. 2019.
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