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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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Data corrente: |
11/04/2024 |
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Data da última atualização: |
11/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VAZ, C. S. L.; FONSECA, F. M. da; RECH, D. V.; MORES, M. A. Z.; COLDEBELLA, A.; CANTAO, M. E. |
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Afiliação: |
CLARISSA SILVEIRA LUIZ VAZ, CNPSA; FRANCISCO NOE DA FONSECA, GEM; DAIANE VOSS RECH, CNPSA; MARCOS ANTONIO ZANELLA MORES, CNPSA; ARLEI COLDEBELLA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA. |
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Título: |
Wild-type lytic bacteriophages against Salmonella Heidelberg: further characterization and effect of prophylactic therapy in broiler chickens. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Research in Veterinary Science, v. 171, n. 105247, 2024. |
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DOI: |
https://doi.org/10.1016/j.rvsc.2024.105247 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age. MenosAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous a... Mostrar Tudo |
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Palavras-Chave: |
Análise genômica; Bacteriófago; Foodborne pathogen; Genomic analysis; Patógeno de origem alimentar; Terapia fágica. |
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Thesagro: |
Frango de Corte; Salmonelose. |
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Thesaurus Nal: |
Bacteriophages; Phage therapy; Poultry; Salmonellosis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02834naa a2200337 a 4500
001 2163526
005 2024-04-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.rvsc.2024.105247$2DOI
100 1 $aVAZ, C. S. L.
245 $aWild-type lytic bacteriophages against Salmonella Heidelberg$bfurther characterization and effect of prophylactic therapy in broiler chickens.$h[electronic resource]
260 $c2024
520 $aAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age.
650 $aBacteriophages
650 $aPhage therapy
650 $aPoultry
650 $aSalmonellosis
650 $aFrango de Corte
650 $aSalmonelose
653 $aAnálise genômica
653 $aBacteriófago
653 $aFoodborne pathogen
653 $aGenomic analysis
653 $aPatógeno de origem alimentar
653 $aTerapia fágica
700 1 $aFONSECA, F. M. da
700 1 $aRECH, D. V.
700 1 $aMORES, M. A. Z.
700 1 $aCOLDEBELLA, A.
700 1 $aCANTAO, M. E.
773 $tResearch in Veterinary Science$gv. 171, n. 105247, 2024.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
26/11/2009 |
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Data da última atualização: |
17/11/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
BRIENZA JUNIOR, S.; MONTEIRO, D. C. A.; MARTORANO, L. G.; LISBOA, L. S.; MAESTRI, M. P.; SCHULER, A. |
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Afiliação: |
SILVIO BRIENZA JUNIOR, CPATU; DAIANA C. A. MONTEIRO, Graduanda UFRA; LUCIETA GUERREIRO MARTORANO, CPATU; LEILA S. LISBOA, MPEG; MAYARA P. MAESTRI, Graduanda UFRA; AZENETH EUFRAUSINO SCHULER, CNPS. |
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Título: |
Energetic potential of Sclerobium paniculatum vogel (taxi-branco) and its preferential top climatic condition in Eastern Amazonia, Brasil. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: INTERNATIONAL RESEARCH ON FOOD SECURITY, NATURAL RESOURCE MANAGEMENT AND RURAL DEVELOPMENT, 2009, Hamburg. Biophysical and socio-economic frame conditions for the sustainable management of natural resources. Hamburg: University of Hamburg, 2009. |
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Idioma: |
Inglês |
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Notas: |
Tropentag 2009. |
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Palavras-Chave: |
Brasil; Taxi-branco. |
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Thesagro: |
Climatologia; Sclerolobium paniculatum. |
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Thesaurus NAL: |
Amazonia. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/108759/1/5555.pdf
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Marc: |
LEADER 00935nam a2200229 a 4500
001 1576169
005 2022-11-17
008 2009 bl uuuu u00u1 u #d
100 1 $aBRIENZA JUNIOR, S.
245 $aEnergetic potential of Sclerobium paniculatum vogel (taxi-branco) and its preferential top climatic condition in Eastern Amazonia, Brasil.$h[electronic resource]
260 $aIn: INTERNATIONAL RESEARCH ON FOOD SECURITY, NATURAL RESOURCE MANAGEMENT AND RURAL DEVELOPMENT, 2009, Hamburg. Biophysical and socio-economic frame conditions for the sustainable management of natural resources. Hamburg: University of Hamburg$c2009
500 $aTropentag 2009.
650 $aAmazonia
650 $aClimatologia
650 $aSclerolobium paniculatum
653 $aBrasil
653 $aTaxi-branco
700 1 $aMONTEIRO, D. C. A.
700 1 $aMARTORANO, L. G.
700 1 $aLISBOA, L. S.
700 1 $aMAESTRI, M. P.
700 1 $aSCHULER, A.
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Embrapa Amazônia Oriental (CPATU) |
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