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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
30/11/2023 |
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Data da última atualização: |
30/11/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, H. M.; NICIURA, S. C. M.; NOGUEIRA, A. R. de A. |
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Afiliação: |
HERICK MACEDO SANTOS, Federal University of São Carlos; SIMONE CRISTINA MEO NICIURA, CPPSE; ANA RITA DE ARAUJO NOGUEIRA, CPPSE. |
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Título: |
Analytical strategies and method validation to access the bioavailability of essential elements and arsenic from raw and cooked fish and shrimp. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Food Science & Technology, v. 3, n. 7, 2023. |
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Páginas: |
p. 1229-1237. |
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DOI: |
10.1021/acsfoodscitech.3c00132 |
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Idioma: |
Inglês |
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Conteúdo: |
The bioavailability of essential and toxic elements in raw/cooked tilapia and shrimp was estimated using in vitro gastrointestinal digestion followed by the Caco-2 cell model to simulate the human intestinal barrier. Analytical strategies such as aerosol dilution, collision cell, and matrix-matching calibration were successfully explored for multielement quantification using ICP OES and ICP-MS. From Caco-2 cell bioassays, the estimated bioavailability values ranged from 1.4% (Fe) to 15.2% (Cu) in tilapia and from 0.2% (Fe) to 10.7% (P) in shrimp. Air-frying and oven-baking methods decreased tilapia’s Mg, P, S, Cu, and Fe bioavailability. Contrarily, the bioavailability of essential elements (except Mn) in shrimp seemed to increase after air-frying. Arsenic bioavailability in cooked shrimp was significantly higher than in the raw sample (1.9 vs 5.7%) but fortunately below the limit allowed by legislation. In spite of the complexity of basal solutions from the cell-based bioassays, the applied analytical strategies allowed the accurate quantification of the bioavailable fractions by ICP-based methods. |
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Palavras-Chave: |
Analytical strategies; Caco-2 cells; In vitro uptake; Thermal treatments. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 01841naa a2200217 a 4500
001 2158958
005 2023-11-30
008 2023 bl uuuu u00u1 u #d
024 7 $a10.1021/acsfoodscitech.3c00132$2DOI
100 1 $aSANTOS, H. M.
245 $aAnalytical strategies and method validation to access the bioavailability of essential elements and arsenic from raw and cooked fish and shrimp.$h[electronic resource]
260 $c2023
300 $ap. 1229-1237.
520 $aThe bioavailability of essential and toxic elements in raw/cooked tilapia and shrimp was estimated using in vitro gastrointestinal digestion followed by the Caco-2 cell model to simulate the human intestinal barrier. Analytical strategies such as aerosol dilution, collision cell, and matrix-matching calibration were successfully explored for multielement quantification using ICP OES and ICP-MS. From Caco-2 cell bioassays, the estimated bioavailability values ranged from 1.4% (Fe) to 15.2% (Cu) in tilapia and from 0.2% (Fe) to 10.7% (P) in shrimp. Air-frying and oven-baking methods decreased tilapia’s Mg, P, S, Cu, and Fe bioavailability. Contrarily, the bioavailability of essential elements (except Mn) in shrimp seemed to increase after air-frying. Arsenic bioavailability in cooked shrimp was significantly higher than in the raw sample (1.9 vs 5.7%) but fortunately below the limit allowed by legislation. In spite of the complexity of basal solutions from the cell-based bioassays, the applied analytical strategies allowed the accurate quantification of the bioavailable fractions by ICP-based methods.
653 $aAnalytical strategies
653 $aCaco-2 cells
653 $aIn vitro uptake
653 $aThermal treatments
700 1 $aNICIURA, S. C. M.
700 1 $aNOGUEIRA, A. R. de A.
773 $tFood Science & Technology$gv. 3, n. 7, 2023.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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