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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
01/06/2023 |
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Data da última atualização: |
01/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DORTA, S. DE O.; ATTÍLIO, L. B.; ZANARDI, O. Z.; LOPES, J. R. S.; MACHADO, M. A.; ASTUA, J. de F. |
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Afiliação: |
SÍLVIA DE OLIVEIRA DORTA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; LÍSIA BORGES ATTÍLIO, INSTITUTO AGRONÔMICO DE CAMPINAS; ODIMAR ZANUZO ZANARDI, INSTITUTO FEDERAL DE SANTA CATARINA; JOÃO ROBERTO SPOTTI LOPES, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; MARCOS ANTONIO MACHADO, INSTITUTO AGRONÔMICO DE CAMPINAS; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Genetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera: Liviidae). |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of Biotechnolog, v.68, May, p.60-70, 2023. |
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ISSN: |
0168-1656 |
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DOI: |
https://doi.org/10.1016/j.jbiotec.2023.04.007 |
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Idioma: |
Inglês |
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Conteúdo: |
The Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the vector of Candidatus Liberibacter spp., the bacteria associated with huanglongbing (HLB), the most devastating disease of citrus worldwide. HLB management has heavily relied on insecticide applications to control the ACP, although there are efforts towards more sustainable alternatives. In previous work, our group assessed the potential bioactivity of different strains of Bacillus thuringiensis (Eubacteriales: Bacillaceae) (Bt) containing cry/cyt genes as feasible tools to control ACP nymphs. Here, we report an attempt to use the cry11A gene from Bt to produce transgenic sweet orange plants using two promoters. For the genetic transformation, ?Hamlin? and ?Valencia? sweet orange seedlings were used as sources of explants. Transgenic plants were detected by polymerase chain reaction (PCR) with specific primers, and the transgene copy number was confirmed by Southern blot analyses. Transcript expression levels were determined by qPCR. Mortality assays of D. citri nymphs were carried out in a greenhouse, and the effect of the events tested ranged from 22% to 43% at the end of the five-day exposure period. To our knowledge, this is the first manuscript reporting the production of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs. |
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Thesagro: |
Laranja; Melhoramento Vegetal. |
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Thesaurus Nal: |
Oranges. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02204naa a2200241 a 4500
001 2154159
005 2023-06-01
008 2023 bl uuuu u00u1 u #d
022 $a0168-1656
024 7 $ahttps://doi.org/10.1016/j.jbiotec.2023.04.007$2DOI
100 1 $aDORTA, S. DE O.
245 $aGenetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera$bLiviidae).$h[electronic resource]
260 $c2023
520 $aThe Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the vector of Candidatus Liberibacter spp., the bacteria associated with huanglongbing (HLB), the most devastating disease of citrus worldwide. HLB management has heavily relied on insecticide applications to control the ACP, although there are efforts towards more sustainable alternatives. In previous work, our group assessed the potential bioactivity of different strains of Bacillus thuringiensis (Eubacteriales: Bacillaceae) (Bt) containing cry/cyt genes as feasible tools to control ACP nymphs. Here, we report an attempt to use the cry11A gene from Bt to produce transgenic sweet orange plants using two promoters. For the genetic transformation, ?Hamlin? and ?Valencia? sweet orange seedlings were used as sources of explants. Transgenic plants were detected by polymerase chain reaction (PCR) with specific primers, and the transgene copy number was confirmed by Southern blot analyses. Transcript expression levels were determined by qPCR. Mortality assays of D. citri nymphs were carried out in a greenhouse, and the effect of the events tested ranged from 22% to 43% at the end of the five-day exposure period. To our knowledge, this is the first manuscript reporting the production of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs.
650 $aOranges
650 $aLaranja
650 $aMelhoramento Vegetal
700 1 $aATTÍLIO, L. B.
700 1 $aZANARDI, O. Z.
700 1 $aLOPES, J. R. S.
700 1 $aMACHADO, M. A.
700 1 $aASTUA, J. de F.
773 $tJournal of Biotechnolog$gv.68, May, p.60-70, 2023.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
28/11/2022 |
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Data da última atualização: |
12/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEREIRA, R. A. A.; SILVA, H. F. M.; GONÇALVES, A. O.; VIANNA, M. S.; SILVA, T. J. A.; FENNER, W.; VIEIRA, P. V. D.; MARIN, F. R. |
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Afiliação: |
RODOLFO A. A. PEREIRA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; EVANDRO H. F. M. SILVA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; ALEXANDRE ORTEGA GONCALVES, CNPS; MURILO S. VIANNA, UNIVERSITY OF LEEDS; TONNY J. A. SILVA, UNIVERSIDADE FEDERAL DE RONDONÓPOLIS; WILLIAM FENNER, UNIVERSIDADE FEDERAL DE MATO GROSSO; PAULO V. D. VIEIRA, UNIVERSIDADE ESTADUAL DE MARINGÁ; FÁBIO R. MARIN, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ. |
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Título: |
Winter wheat evapotranspiration and irrigation requirements across tropical and sub-tropical producing regions in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Theoretical and Applied Climatology, v. 151, n. 1/2, p. 375-388, Jan. 2023. |
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DOI: |
https://doi.org/10.1007/s00704-022-04282-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Agricultural intensification is often seen as an appropriate approach to meet the growing demand for agricultural products. In regions affected by seasonal or chronic water scarcity, closing the yield gap depends on irrigation. Brazil is the second-largest wheat importer of the world and irrigated wheat has high yield potential in Brazil, especially in tropical and sub-tropical regions of the country. The crop coefficient (Kc) is still a practical and simple way of quantifying crop irrigation requirements. In this paper, we provided Kc values based on a robust experimental dataset across different wheat-producing regions in Brazil. Four experiments were conducted in three sites [season 2017 in Piracicaba, State of São Paulo (Southeast region); season 2012 Maringá, State of Paraná (Southern region); and seasons 2016 and 2017 Rondonópolis, State of Mato Grosso) (Midwest region)] using lysimeters and the Bowen ratio energy balance method for crop evapotranspiration measurements. Our data showed that Kc values varied among the regions analyzed. For the Midwest region, Kc values ranged from 0.88 to 1.36; the Southeastern region from 0.81 to 1.15, and the Southern region from 0.67 to 1.01. Considering values suggested by FAO, those for arid climates should be used in the Midwest region, sub-humid values for the Southeast, and humid values in the Southern. Differently, that was observed for other crops, our database showed wheat Kc values being stable disregarding the ETo range, which might be related to relatively lower ETo during the winter. MenosAgricultural intensification is often seen as an appropriate approach to meet the growing demand for agricultural products. In regions affected by seasonal or chronic water scarcity, closing the yield gap depends on irrigation. Brazil is the second-largest wheat importer of the world and irrigated wheat has high yield potential in Brazil, especially in tropical and sub-tropical regions of the country. The crop coefficient (Kc) is still a practical and simple way of quantifying crop irrigation requirements. In this paper, we provided Kc values based on a robust experimental dataset across different wheat-producing regions in Brazil. Four experiments were conducted in three sites [season 2017 in Piracicaba, State of São Paulo (Southeast region); season 2012 Maringá, State of Paraná (Southern region); and seasons 2016 and 2017 Rondonópolis, State of Mato Grosso) (Midwest region)] using lysimeters and the Bowen ratio energy balance method for crop evapotranspiration measurements. Our data showed that Kc values varied among the regions analyzed. For the Midwest region, Kc values ranged from 0.88 to 1.36; the Southeastern region from 0.81 to 1.15, and the Southern region from 0.67 to 1.01. Considering values suggested by FAO, those for arid climates should be used in the Midwest region, sub-humid values for the Southeast, and humid values in the Southern. Differently, that was observed for other crops, our database showed wheat Kc values being stable disregarding the ETo range, wh... Mostrar Tudo |
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Thesagro: |
Evapotranspiração; Irrigação; Trigo. |
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Thesaurus NAL: |
Evapotranspiration; Irrigation; Wheat. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02464naa a2200289 a 4500
001 2148828
005 2023-01-12
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00704-022-04282-3$2DOI
100 1 $aPEREIRA, R. A. A.
245 $aWinter wheat evapotranspiration and irrigation requirements across tropical and sub-tropical producing regions in Brazil.$h[electronic resource]
260 $c2023
520 $aAgricultural intensification is often seen as an appropriate approach to meet the growing demand for agricultural products. In regions affected by seasonal or chronic water scarcity, closing the yield gap depends on irrigation. Brazil is the second-largest wheat importer of the world and irrigated wheat has high yield potential in Brazil, especially in tropical and sub-tropical regions of the country. The crop coefficient (Kc) is still a practical and simple way of quantifying crop irrigation requirements. In this paper, we provided Kc values based on a robust experimental dataset across different wheat-producing regions in Brazil. Four experiments were conducted in three sites [season 2017 in Piracicaba, State of São Paulo (Southeast region); season 2012 Maringá, State of Paraná (Southern region); and seasons 2016 and 2017 Rondonópolis, State of Mato Grosso) (Midwest region)] using lysimeters and the Bowen ratio energy balance method for crop evapotranspiration measurements. Our data showed that Kc values varied among the regions analyzed. For the Midwest region, Kc values ranged from 0.88 to 1.36; the Southeastern region from 0.81 to 1.15, and the Southern region from 0.67 to 1.01. Considering values suggested by FAO, those for arid climates should be used in the Midwest region, sub-humid values for the Southeast, and humid values in the Southern. Differently, that was observed for other crops, our database showed wheat Kc values being stable disregarding the ETo range, which might be related to relatively lower ETo during the winter.
650 $aEvapotranspiration
650 $aIrrigation
650 $aWheat
650 $aEvapotranspiração
650 $aIrrigação
650 $aTrigo
700 1 $aSILVA, H. F. M.
700 1 $aGONÇALVES, A. O.
700 1 $aVIANNA, M. S.
700 1 $aSILVA, T. J. A.
700 1 $aFENNER, W.
700 1 $aVIEIRA, P. V. D.
700 1 $aMARIN, F. R.
773 $tTheoretical and Applied Climatology$gv. 151, n. 1/2, p. 375-388, Jan. 2023.
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