|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
21/01/2022 |
Data da última atualização: |
14/02/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, A. K. A.; ECHEVERRI, L. M. S.; SOUSA, B. A. de; VICENTE, M. C. de; NASCIMENTO, S. C. do; SILVA, G. F. da; FERREIRA JÚNIOR, A.; LAU, D.; BOGO, A.; SILVA, F. N. da. |
Afiliação: |
ANA KAROLINY ALVES SANTOS, UDESC/Lages; LINA MARIA SALAZAR ECHEVERRI, UDESC/Lages; BRUNA ANDERSEN DE SOUSA, UDESC/Lages; MONICA CHACON DE VICENTE, UFG; SAMARA CAMPOS DO NASCIMENTO, UDESC/Lages; GUSTAVO FELIPPE DA SILVA, UDESC/Lages; ÁLVARO FERREIRA JÚNIOR, UFG; DOUGLAS LAU, CNPT; AMAURI BOGO, UDESC/Lages; FABIO NASCIMENTO DA SILVA, UDESC/Lages. |
Título: |
Production and characterization of chicken IgY for detecting wheat stripe mosaic virus. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Tropical Plant Pathology, 11 Jan. 2022. |
DOI: |
https://doi.org/10.1007/s40858-021-00481-x |
Idioma: |
Inglês |
Conteúdo: |
Abstract: In Brazil, wheat (Triticum aestivum) is cultivated mainly in the southern part. The soilborne wheat mosaic disease (SBWMD), caused by the wheat stripe mosaic virus (WhSMV), is one of the most important viruses in the region. Its diagnosis is based mainly on symptoms since molecular techniques are expensive, especially for RNA genomes viruses. However, serological tests using IgY immunoglobulins are highly efective in identifying viruses. This study aimed to produce and characterize a polyclonal antibody capable of interacting with the WhSMV capsid protein. The gene encoding the virus capsid protein was inserted into the expression vector pET-15b; the transformation and expression occurred in Escherichia coli BL21::DE3 in vitro. The recombinant protein was inoculated in laying hens (Gallus gallus domesticus). The anti-WhSMV IgY antibodies were extracted from the chicken yolks. The IgY was the basis for immuno-assays such as indirect Enzyme-linked immunosorbent assay (ELISA) and Western blot, targeting the purifed capsid protein or the whole virus. Also, a PCR test was performed with samples of root and leaves of infected plants, confrming the presence of the virus in both tissues. The PCR results were in agreement with the ELISA tests performed with all samples. IgY produced in chickens specifcally recognized the purifed viral protein and the WhSMV present in the extract from leaves of WhSMV-infected plants. |
Palavras-Chave: |
Benyviridae; Capsid protein; Criação de frango; Diagnosis; IgY; WhSMV. |
Thesagro: |
Diagnostico; Mosaico; Trigo; Vírus. |
Thesaurus Nal: |
Chickens; Triticum aestivum WIS-2 virus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02500naa a2200385 a 4500 001 2139239 005 2022-02-14 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s40858-021-00481-x$2DOI 100 1 $aSANTOS, A. K. A. 245 $aProduction and characterization of chicken IgY for detecting wheat stripe mosaic virus.$h[electronic resource] 260 $c2022 520 $aAbstract: In Brazil, wheat (Triticum aestivum) is cultivated mainly in the southern part. The soilborne wheat mosaic disease (SBWMD), caused by the wheat stripe mosaic virus (WhSMV), is one of the most important viruses in the region. Its diagnosis is based mainly on symptoms since molecular techniques are expensive, especially for RNA genomes viruses. However, serological tests using IgY immunoglobulins are highly efective in identifying viruses. This study aimed to produce and characterize a polyclonal antibody capable of interacting with the WhSMV capsid protein. The gene encoding the virus capsid protein was inserted into the expression vector pET-15b; the transformation and expression occurred in Escherichia coli BL21::DE3 in vitro. The recombinant protein was inoculated in laying hens (Gallus gallus domesticus). The anti-WhSMV IgY antibodies were extracted from the chicken yolks. The IgY was the basis for immuno-assays such as indirect Enzyme-linked immunosorbent assay (ELISA) and Western blot, targeting the purifed capsid protein or the whole virus. Also, a PCR test was performed with samples of root and leaves of infected plants, confrming the presence of the virus in both tissues. The PCR results were in agreement with the ELISA tests performed with all samples. IgY produced in chickens specifcally recognized the purifed viral protein and the WhSMV present in the extract from leaves of WhSMV-infected plants. 650 $aChickens 650 $aTriticum aestivum WIS-2 virus 650 $aDiagnostico 650 $aMosaico 650 $aTrigo 650 $aVírus 653 $aBenyviridae 653 $aCapsid protein 653 $aCriação de frango 653 $aDiagnosis 653 $aIgY 653 $aWhSMV 700 1 $aECHEVERRI, L. M. S. 700 1 $aSOUSA, B. A. de 700 1 $aVICENTE, M. C. de 700 1 $aNASCIMENTO, S. C. do 700 1 $aSILVA, G. F. da 700 1 $aFERREIRA JÚNIOR, A. 700 1 $aLAU, D. 700 1 $aBOGO, A. 700 1 $aSILVA, F. N. da 773 $tTropical Plant Pathology, 11 Jan. 2022.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Trigo (CNPT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
16/12/2021 |
Data da última atualização: |
23/02/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
PIRES, D. A.; TACCA, L. M. R. A.; ASLAN, J. E.; MURAD, A. M.; NASCIMENTO, C. J.; BARBOSA, E. A.; BLOCH JUNIOR, C. |
Afiliação: |
DIEGO A. T. PIRES, IFG, Luziânia; LUISA M. R. A. TACCA; JOSEPH E. ASLAN, Oregon Health & Science University, USA; ANDRE MELRO MURAD, Cenargen; CLAUDIA J. NASCIMENTO, UNIRIO; EDER A. BARBOSA; CARLOS BLOCH JUNIOR, Cenargen. |
Título: |
Novel disintegrin-like peptides derived from an amphibian skin cDNA sequence of Hypsiboas punctatus. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Journal of Peptide Science, e3382, 2021. |
DOI: |
10.1002/psc.3382 |
Idioma: |
Inglês |
Notas: |
Na publicação: Carlos Bloch Jr. |
Palavras-Chave: |
Amphibian; Disintegrin motifs; Peptide. |
Thesaurus NAL: |
Platelet activation. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00769naa a2200253 a 4500 001 2137931 005 2022-02-23 008 2021 bl uuuu u00u1 u #d 024 7 $a10.1002/psc.3382$2DOI 100 1 $aPIRES, D. A. 245 $aNovel disintegrin-like peptides derived from an amphibian skin cDNA sequence of Hypsiboas punctatus.$h[electronic resource] 260 $c2021 500 $aNa publicação: Carlos Bloch Jr. 650 $aPlatelet activation 653 $aAmphibian 653 $aDisintegrin motifs 653 $aPeptide 700 1 $aTACCA, L. M. R. A. 700 1 $aASLAN, J. E. 700 1 $aMURAD, A. M. 700 1 $aNASCIMENTO, C. J. 700 1 $aBARBOSA, E. A. 700 1 $aBLOCH JUNIOR, C. 773 $tJournal of Peptide Science, e3382, 2021.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|