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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
15/10/2020 |
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Data da última atualização: |
15/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TADINI, A. M.; MOUNIER, S.; MILORI, D. M. B. P. |
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Afiliação: |
DEBORA MARCONDES BASTOS PEREIRA, CNPDIA. |
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Título: |
Modeling the quenching of fluorescence from organic matter in Amazonian soils. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Science of the Total Environment, v. 698, 134067, 2020. |
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ISSN: |
0048-9697 |
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DOI: |
https://doi.org/10.1016/j.scitotenv.2019.134067 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Amazonian Spodosol; Complexation; Humic fractions; Mobility. |
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Categoria do assunto: |
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Marc: |
LEADER 00643naa a2200205 a 4500
001 2125518
005 2020-10-15
008 2020 bl uuuu u00u1 u #d
022 $a0048-9697
024 7 $ahttps://doi.org/10.1016/j.scitotenv.2019.134067$2DOI
100 1 $aTADINI, A. M.
245 $aModeling the quenching of fluorescence from organic matter in Amazonian soils.$h[electronic resource]
260 $c2020
653 $aAmazonian Spodosol
653 $aComplexation
653 $aHumic fractions
653 $aMobility
700 1 $aMOUNIER, S.
700 1 $aMILORI, D. M. B. P.
773 $tScience of the Total Environment$gv. 698, 134067, 2020.
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Embrapa Instrumentação (CNPDIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
18/01/2023 |
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Data da última atualização: |
26/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
TRICHEZ, D.; STEINDORFF, A. S.; MORAIS JÚNIOR, W. G. de; VILELA, N.; BERGMANN, J. C.; FORMIGHIERI, E. F.; GONCALVES, S. B.; ALMEIDA, J. R. M. de. |
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Afiliação: |
DÉBORA TRICHEZ, CNPAE; ANDREI S. STEINDORFF, CNPAE/ US DOE JOINT GENOME INSTITUTE; WILSON G. DE MORAIS JÚNIOR, CNPAE/ MILHOUSE INTERNATIONAL PTY; NATHÁLIA VILELA, CNPAE; JESSICA CARVALHO BERGMANN; EDUARDO FERNANDES FORMIGHIERI, CNPAE; SILVIA BELEM GONCALVES, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
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Título: |
Identification of traits to improve co-assimilation of glucose and xylose by adaptive evolution of Spathaspora passalidarum and Scheffersomyces stipitis yeasts. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, n. 107, p. 1143?1157, Feb. 2023. |
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DOI: |
https://doi.org/10.1007/s00253-023-12362-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Lignocellulosic biomass is a renewable raw material for producing several high-value-added chemicals and fuels. In general, xylose and glucose are the major sugars in biomass hydrolysates, and their efficient utilization by microorganisms is critical for an economical production process. Yeasts capable of co-consuming mixed sugars might lead to higher yields and productivities in industrial fermentation processes. Herein, we performed adaptive evolution assays with two xylose-fermenting yeasts, Spathaspora passalidarum and Scheffersomyces stipitis, to obtain derived clones with improved capabilities of glucose and xylose co-consumption. Adapted strains were obtained after successive growth selection using xylose and the non-metabolized glucose analog 2-deoxy-D-glucose as a selective pressure. The co-fermentation capacity of evolved and parental strains was evaluated on xylose-glucose mixtures. Our results revealed an improved co-assimilation capability by the evolved strains; however, xylose and glucose consumption were observed at slower rates than the parental yeasts. Genome resequencing of the evolved strains revealed genes affected by non-synonymous variants that might be involved with the co-consumption phenotype, including the HXT2.4 gene that encodes a putative glucose transporter in Sp. passalidarum. Expression of this mutant HXT2.4 in Saccharomyces cerevisiae improved the cells? co-assimilation of glucose and xylose. Therefore, our results demonstrated the successful improvement of co-fermentation through evolutionary engineering and the identification of potential targets for further genetic engineering of different yeast strains. MenosLignocellulosic biomass is a renewable raw material for producing several high-value-added chemicals and fuels. In general, xylose and glucose are the major sugars in biomass hydrolysates, and their efficient utilization by microorganisms is critical for an economical production process. Yeasts capable of co-consuming mixed sugars might lead to higher yields and productivities in industrial fermentation processes. Herein, we performed adaptive evolution assays with two xylose-fermenting yeasts, Spathaspora passalidarum and Scheffersomyces stipitis, to obtain derived clones with improved capabilities of glucose and xylose co-consumption. Adapted strains were obtained after successive growth selection using xylose and the non-metabolized glucose analog 2-deoxy-D-glucose as a selective pressure. The co-fermentation capacity of evolved and parental strains was evaluated on xylose-glucose mixtures. Our results revealed an improved co-assimilation capability by the evolved strains; however, xylose and glucose consumption were observed at slower rates than the parental yeasts. Genome resequencing of the evolved strains revealed genes affected by non-synonymous variants that might be involved with the co-consumption phenotype, including the HXT2.4 gene that encodes a putative glucose transporter in Sp. passalidarum. Expression of this mutant HXT2.4 in Saccharomyces cerevisiae improved the cells? co-assimilation of glucose and xylose. Therefore, our results demonstrated the successfu... Mostrar Tudo |
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Thesaurus NAL: |
Fermentation; Glucose; Sugar products. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02532naa a2200253 a 4500
001 2151042
005 2023-10-26
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00253-023-12362-1$2DOI
100 1 $aTRICHEZ, D.
245 $aIdentification of traits to improve co-assimilation of glucose and xylose by adaptive evolution of Spathaspora passalidarum and Scheffersomyces stipitis yeasts.$h[electronic resource]
260 $c2023
520 $aLignocellulosic biomass is a renewable raw material for producing several high-value-added chemicals and fuels. In general, xylose and glucose are the major sugars in biomass hydrolysates, and their efficient utilization by microorganisms is critical for an economical production process. Yeasts capable of co-consuming mixed sugars might lead to higher yields and productivities in industrial fermentation processes. Herein, we performed adaptive evolution assays with two xylose-fermenting yeasts, Spathaspora passalidarum and Scheffersomyces stipitis, to obtain derived clones with improved capabilities of glucose and xylose co-consumption. Adapted strains were obtained after successive growth selection using xylose and the non-metabolized glucose analog 2-deoxy-D-glucose as a selective pressure. The co-fermentation capacity of evolved and parental strains was evaluated on xylose-glucose mixtures. Our results revealed an improved co-assimilation capability by the evolved strains; however, xylose and glucose consumption were observed at slower rates than the parental yeasts. Genome resequencing of the evolved strains revealed genes affected by non-synonymous variants that might be involved with the co-consumption phenotype, including the HXT2.4 gene that encodes a putative glucose transporter in Sp. passalidarum. Expression of this mutant HXT2.4 in Saccharomyces cerevisiae improved the cells? co-assimilation of glucose and xylose. Therefore, our results demonstrated the successful improvement of co-fermentation through evolutionary engineering and the identification of potential targets for further genetic engineering of different yeast strains.
650 $aFermentation
650 $aGlucose
650 $aSugar products
700 1 $aSTEINDORFF, A. S.
700 1 $aMORAIS JÚNIOR, W. G. de
700 1 $aVILELA, N.
700 1 $aBERGMANN, J. C.
700 1 $aFORMIGHIERI, E. F.
700 1 $aGONCALVES, S. B.
700 1 $aALMEIDA, J. R. M. de
773 $tApplied Microbiology and Biotechnology$gn. 107, p. 1143?1157, Feb. 2023.
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