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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
04/07/2019 |
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Data da última atualização: |
04/07/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAITANO-BERTOLACCI, M. A. B.; BASTOS, R. R. P.; SANTOS, L. R. dos; SOARES, C. O.; RIEGER, J. S. G.; MANTOVANI, C.; SANTANA, M. de S.; ROSINHA, G. M. S. |
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Afiliação: |
Universidade Federal de Mato Grosso do Sul/FUFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia; LENITA RAMIRES DOS SANTOS, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
DNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 2, gmr18295, 2019 |
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Idioma: |
Inglês |
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Conteúdo: |
VIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellular immune responses, but not enough to induce significant protection in the immunized mice. However, given the response in this system, further investigations using the virB9 and virB12 genes of Brucella spp., together with different immune modulators, are warranted. An effort should be made to direct and enhance the immune response, in order to identify a combination that stimulates a better immune response and, consequently, a better level of protection. MenosVIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellu... Mostrar Tudo |
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Palavras-Chave: |
DNA vaccine; T4SS; VirB operon; VirB12; VirB9. |
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Thesagro: |
Brucella Abortus. |
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Thesaurus Nal: |
Bovine brucellosis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02847naa a2200289 a 4500
001 2110376
005 2019-07-04
008 2019 bl uuuu u00u1 u #d
100 1 $aCAITANO-BERTOLACCI, M. A. B.
245 $aDNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice.$h[electronic resource]
260 $c2019
520 $aVIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellular immune responses, but not enough to induce significant protection in the immunized mice. However, given the response in this system, further investigations using the virB9 and virB12 genes of Brucella spp., together with different immune modulators, are warranted. An effort should be made to direct and enhance the immune response, in order to identify a combination that stimulates a better immune response and, consequently, a better level of protection.
650 $aBovine brucellosis
650 $aBrucella Abortus
653 $aDNA vaccine
653 $aT4SS
653 $aVirB operon
653 $aVirB12
653 $aVirB9
700 1 $aBASTOS, R. R. P.
700 1 $aSANTOS, L. R. dos
700 1 $aSOARES, C. O.
700 1 $aRIEGER, J. S. G.
700 1 $aMANTOVANI, C.
700 1 $aSANTANA, M. de S.
700 1 $aROSINHA, G. M. S.
773 $tGenetics and Molecular Research$gv. 18, n. 2, gmr18295, 2019
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Unidades Centrais. |
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Data corrente: |
12/12/1995 |
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Data da última atualização: |
22/01/2021 |
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Autoria: |
POLTRONIERI, M. C.; DUARTE, M. de L. R.; RODRIGUES, J. E. L. F.; NAZARE, R. F. R. de; KATO, A. K.; OLIVEIRA, A. F. F. de. |
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Afiliação: |
MARLI COSTA POLTRONIERI, CPATU; MARIA DE LOURDES REIS DUARTE, CPATU; JOAO ELIAS LOPES F RODRIGUES, CPATU; RAIMUNDA FATIMA RIBEIRO DE NAZARE, CPATU; ARMANDO KOUZO KATO, CPATU; ARISTOTELES FERNANDO F DE OLIVEIRA, CPATU. |
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Título: |
A cultura do guaraná. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Brasília, DF: Embrapa-SPI; Belém, PA: Embrapa-CPATU, 1995. |
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Páginas: |
48 p. |
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Descrição Física: |
il. |
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Série: |
(Coleção plantar, 29). |
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Idioma: |
Português |
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Conteúdo: |
Clima e solo; Variedades; Formacao de mudas; Plantio; Tratosw culturais; Adubacao; Controle de pragas e doencas; Floracao e frutificacao; Colheita e beneficiamento; Composicao da semente; Coeficientes de producao. |
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Palavras-Chave: |
Brasil; Cultivation; Cultivo; Disease; Doenças; Fertilization; Fruit; Paullinia cupana var; Plant diseases; Pragas; Production; sorbilis. |
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Thesagro: |
Adubação; Beneficiamento; Clima; Colheita; Doença; Fruta; Guaraná; Muda; Paullinia Cupana; Praga; Produção; Semente; Solo; Variedade. |
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Thesaurus NAL: |
Amazonia; climate; fertilizer application; harvesting; pests; pruning; seeds; soil; varieties. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162284/1/A-cultura-do-guarana.pdf
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Marc: |
LEADER 01646nam a2200613 a 4500
001 1374475
005 2021-01-22
008 1995 bl uuuu u0uu1 u #d
100 1 $aPOLTRONIERI, M. C.
245 $aA cultura do guaraná.
260 $aBrasília, DF: Embrapa-SPI; Belém, PA: Embrapa-CPATU$c1995
300 $a48 p.$cil.
490 $a(Coleção plantar, 29).
520 $aClima e solo; Variedades; Formacao de mudas; Plantio; Tratosw culturais; Adubacao; Controle de pragas e doencas; Floracao e frutificacao; Colheita e beneficiamento; Composicao da semente; Coeficientes de producao.
650 $aAmazonia
650 $aclimate
650 $afertilizer application
650 $aharvesting
650 $apests
650 $apruning
650 $aseeds
650 $asoil
650 $avarieties
650 $aAdubação
650 $aBeneficiamento
650 $aClima
650 $aColheita
650 $aDoença
650 $aFruta
650 $aGuaraná
650 $aMuda
650 $aPaullinia Cupana
650 $aPraga
650 $aProdução
650 $aSemente
650 $aSolo
650 $aVariedade
653 $aBrasil
653 $aCultivation
653 $aCultivo
653 $aDisease
653 $aDoenças
653 $aFertilization
653 $aFruit
653 $aPaullinia cupana var
653 $aPlant diseases
653 $aPragas
653 $aProduction
653 $asorbilis
700 1 $aDUARTE, M. de L. R.
700 1 $aRODRIGUES, J. E. L. F.
700 1 $aNAZARE, R. F. R. de
700 1 $aKATO, A. K.
700 1 $aOLIVEIRA, A. F. F. de
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