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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
29/11/2018 |
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Data da última atualização: |
30/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OKINO, C. H.; GIGLIOTI, R.; Silva, P. C.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; Rodrigo Giglioti, UNESP; Pamella Cristini Silva, UNICEP; Henrique Nunes de Oliveira, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Comparative evaluation of DNA extraction kit, matrix sample and qPCR assays for bovine babesiosis monitoring. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 45, n. 6, p. 2671-2680, 2018. |
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DOI: |
10.1007/s11033-018-4436-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Bovine babesiosis caused by protozoan parasites Babesia bovis and B. bigemina is one of the most important causes of losses for the livestock industry in tropical and subtropical regions of the world. Therefore, highly sensitive and specific tools for these hemoparasites detection and monitoring are required, especially in carrier animals, in which low parasite levels were usually present. In this context, qPCR assays have been successfully and fairly used in last years. Aiming to improve the performance of Babesia levels monitoring by qPCR, some of main aspects of this methodology that may influence results were tested: DNA extraction kits, whole blood EDTA pre-treatment, blood source (tip of tail or jugular vein), erythrocytes isolation, FTA card interference and qPCR system of detection. Under our experimental conditions, both EDTA pre-treatment and FTA card application have no influence on the sensitivity of detection, and two DNA extraction kits provided higher sensitivity compared to others. As expected, blood samples collected from the tip of tail vessels presented higher levels of B. bovis DNA compared to those obtained from the jugular vein, and erythrocytes processed isolated has also improved the sensitivity compared to whole blood. Moreover, both qPCR assays here developed using hydrolysis probes for B. bovis and B. bigemina detection, presented enhanced reproducibility compared to qPCR assays using intercalating dye system. Even, qPCR for B. bigemina using hydrolysis probe here developed presented higher sensitivity compared to intercalating dye system. This study has contributed to the improvement of molecular diagnosis of bovine babesiosis, which may improve epidemiological studies related to these pathogens. MenosBovine babesiosis caused by protozoan parasites Babesia bovis and B. bigemina is one of the most important causes of losses for the livestock industry in tropical and subtropical regions of the world. Therefore, highly sensitive and specific tools for these hemoparasites detection and monitoring are required, especially in carrier animals, in which low parasite levels were usually present. In this context, qPCR assays have been successfully and fairly used in last years. Aiming to improve the performance of Babesia levels monitoring by qPCR, some of main aspects of this methodology that may influence results were tested: DNA extraction kits, whole blood EDTA pre-treatment, blood source (tip of tail or jugular vein), erythrocytes isolation, FTA card interference and qPCR system of detection. Under our experimental conditions, both EDTA pre-treatment and FTA card application have no influence on the sensitivity of detection, and two DNA extraction kits provided higher sensitivity compared to others. As expected, blood samples collected from the tip of tail vessels presented higher levels of B. bovis DNA compared to those obtained from the jugular vein, and erythrocytes processed isolated has also improved the sensitivity compared to whole blood. Moreover, both qPCR assays here developed using hydrolysis probes for B. bovis and B. bigemina detection, presented enhanced reproducibility compared to qPCR assays using intercalating dye system. Even, qPCR for B. bigemina using hydro... Mostrar Tudo |
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Palavras-Chave: |
DNA extraction; Hydrolysis probe; Matrix sample; QPCR. |
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Thesagro: |
Babesia Bovis. |
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Thesaurus Nal: |
Babesiosis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02517naa a2200253 a 4500
001 2100307
005 2018-11-30
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s11033-018-4436-9$2DOI
100 1 $aOKINO, C. H.
245 $aComparative evaluation of DNA extraction kit, matrix sample and qPCR assays for bovine babesiosis monitoring.$h[electronic resource]
260 $c2018
520 $aBovine babesiosis caused by protozoan parasites Babesia bovis and B. bigemina is one of the most important causes of losses for the livestock industry in tropical and subtropical regions of the world. Therefore, highly sensitive and specific tools for these hemoparasites detection and monitoring are required, especially in carrier animals, in which low parasite levels were usually present. In this context, qPCR assays have been successfully and fairly used in last years. Aiming to improve the performance of Babesia levels monitoring by qPCR, some of main aspects of this methodology that may influence results were tested: DNA extraction kits, whole blood EDTA pre-treatment, blood source (tip of tail or jugular vein), erythrocytes isolation, FTA card interference and qPCR system of detection. Under our experimental conditions, both EDTA pre-treatment and FTA card application have no influence on the sensitivity of detection, and two DNA extraction kits provided higher sensitivity compared to others. As expected, blood samples collected from the tip of tail vessels presented higher levels of B. bovis DNA compared to those obtained from the jugular vein, and erythrocytes processed isolated has also improved the sensitivity compared to whole blood. Moreover, both qPCR assays here developed using hydrolysis probes for B. bovis and B. bigemina detection, presented enhanced reproducibility compared to qPCR assays using intercalating dye system. Even, qPCR for B. bigemina using hydrolysis probe here developed presented higher sensitivity compared to intercalating dye system. This study has contributed to the improvement of molecular diagnosis of bovine babesiosis, which may improve epidemiological studies related to these pathogens.
650 $aBabesiosis
650 $aBabesia Bovis
653 $aDNA extraction
653 $aHydrolysis probe
653 $aMatrix sample
653 $aQPCR
700 1 $aGIGLIOTI, R.
700 1 $aSilva, P. C.
700 1 $aOLIVEIRA, H. N. de
700 1 $aOLIVEIRA, M. C. de S.
773 $tMolecular Biology Reports$gv. 45, n. 6, p. 2671-2680, 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
18/06/1998 |
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Data da última atualização: |
13/04/2020 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
SILVA, F. L. R. da; ARAUJO, A. M. de. |
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Afiliação: |
CNPC; ADRIANA MELLO DE ARAUJO, CNPC. |
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Título: |
Parâmetros produtivos da raça Somalis no Município de Independência, CE. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Sobral: EMBRAPA-CNPC, 1998. |
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Páginas: |
5 p. |
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Série: |
(EMBRAPA-CNPC. Comunicado Tecnico, 35). |
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Idioma: |
Português |
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Palavras-Chave: |
Brasil; Ceara; Improvement; Independencia; Independencia: Ceara; Parametro produtivo; Performance produtiva; Productive performance; Productivity; Raca Somalis. |
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Thesagro: |
Melhoramento; Ovino; Produtividade. |
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Thesaurus NAL: |
Animal performance; Brazil; sheep. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/26701/1/COT-35.pdf
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Marc: |
LEADER 00875nam a2200325 a 4500
001 1514960
005 2020-04-13
008 1998 bl uuuu u0uu1 u #d
100 1 $aSILVA, F. L. R. da
245 $aParâmetros produtivos da raça Somalis no Município de Independência, CE.
260 $aSobral: EMBRAPA-CNPC$c1998
300 $a5 p.
490 $a(EMBRAPA-CNPC. Comunicado Tecnico, 35).
650 $aAnimal performance
650 $aBrazil
650 $asheep
650 $aMelhoramento
650 $aOvino
650 $aProdutividade
653 $aBrasil
653 $aCeara
653 $aImprovement
653 $aIndependencia
653 $aIndependencia: Ceara
653 $aParametro produtivo
653 $aPerformance produtiva
653 $aProductive performance
653 $aProductivity
653 $aRaca Somalis
700 1 $aARAUJO, A. M. de
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Embrapa Caprinos e Ovinos (CNPC) |
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