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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/07/2018 |
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Data da última atualização: |
26/07/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, J. T. M.; FONSECA, J. F. da; BALARO, M. F. A.; ESTEVES, L. V.; ASCOLI, F. O.; LEITE, R. C.; RIBEIRO, A. C. S.; DELGADO, K. F.; SOUZA-FABJAN, J. M. G.; TORRES FILHO, R. A.; BRANDÃO, F. Z. |
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Afiliação: |
Peixoto, Renato Mesquita Peixoto, Renato Mesquita2, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; Jeferson Ferreira da Fonseca, CNPC; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil. |
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Título: |
Effect of natural mating or laparoscopic artificial insemination in superovulated Santa Inês ewes on superovulatory response, fertility and embryo viability. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Animal Production Science, v. 56, n. 9, p. 1463-1468, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study evaluated the effect of two mating methods (GNM: natural mating or GAI: laparoscopic artificial insemination) on superovulatory response, fertility and embryo yield in superovulated ewes. Fifteen non-pregnant Santa Inês ewes were superovulated and either mated by GNM or GAI in a crossover design. Oestrus was synchronised using intravaginal progestagen sponges for 6 days and on Day 5, 300 IU eCG and 0.0375 mg d-cloprostenol were given. Twelve hours after sponge removal, 0.025 mg gonadotropin-releasing hormone was administered. Superovulation started 48 h after gonadotropin-releasing hormone treatment, using 5 IU/kg follicle-stimulating hormone (pFSH). At the first pFSH dose, new sponges were inserted. At the fifth dose, 0.0375 mg cloprostenol was administered and the sponges were removed. The GNM was mated with rams every 12 h, until the end of oestrus. The ewes of GAI were laparoscopic inseminated with frozen?thawed semen 36 and 48 h after sponge removal. Ultrasonography was performed every 24 h from the beginning of oestrus synchronisation treatment and every 12 h from the second sponge removal to 2 days after the last pFSH dose. Six to seven days after mating, the number of corpora lutea (CL) was evaluated by laparoscopy and the females with >4CL were subjected to embryo collection. The interval from sponge removal to ovulation was shorter (P < 0.05) in the GNM. The overall superovulatory response was 63.3% (19/30), with 60.0% and 66.7% in GNM and GAI, respectively (P > 0.05). The number of recovered structures (6.4 ± 2.4 vs 4.5 ± 3.0), recovery rate (74.0 ± 16.0 vs 52.3 ± 26.5%), number of transferable embryos (3.0 ± 2.9 vs 3.6 ± 2.0) and viability rate (47.2 ± 45.3 vs 77.4 ± 37.1%) did not differ between GAI and GNM (P > 0.05). However, the GAI group showed a higher (P < 0.05) number of unfertilised oocytes (3.1 ± 3.1) and a higher non-fertilisation rate (47.1 ± 45.3%) than the GNM (0.9 ± 2.1 and 11.5 ± 21.5%). The mating method did not affect the superovulatory response, and production of viable embryos although the non-fertilisation rate has been inferior for the AI group. MenosAbstract: This study evaluated the effect of two mating methods (GNM: natural mating or GAI: laparoscopic artificial insemination) on superovulatory response, fertility and embryo yield in superovulated ewes. Fifteen non-pregnant Santa Inês ewes were superovulated and either mated by GNM or GAI in a crossover design. Oestrus was synchronised using intravaginal progestagen sponges for 6 days and on Day 5, 300 IU eCG and 0.0375 mg d-cloprostenol were given. Twelve hours after sponge removal, 0.025 mg gonadotropin-releasing hormone was administered. Superovulation started 48 h after gonadotropin-releasing hormone treatment, using 5 IU/kg follicle-stimulating hormone (pFSH). At the first pFSH dose, new sponges were inserted. At the fifth dose, 0.0375 mg cloprostenol was administered and the sponges were removed. The GNM was mated with rams every 12 h, until the end of oestrus. The ewes of GAI were laparoscopic inseminated with frozen?thawed semen 36 and 48 h after sponge removal. Ultrasonography was performed every 24 h from the beginning of oestrus synchronisation treatment and every 12 h from the second sponge removal to 2 days after the last pFSH dose. Six to seven days after mating, the number of corpora lutea (CL) was evaluated by laparoscopy and the females with >4CL were subjected to embryo collection. The interval from sponge removal to ovulation was shorter (P < 0.05) in the GNM. The overall superovulatory response was 63.3% (19/30), with 60.0% and 66.7% in GNM and GAI,... Mostrar Tudo |
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Palavras-Chave: |
Estrus synchronisation; MOET; Oestrus synchronization; Raça Santa Inês. |
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Thesagro: |
Inseminação Artificial; Ovino; Reprodução Animal; Superovulação; Transferência de Embrião. |
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Thesaurus Nal: |
Artificial insemination; Embryo transfer; Sheep; Superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03386naa a2200397 a 4500
001 2093593
005 2018-07-26
008 2016 bl uuuu u00u1 u #d
100 1 $aLIMA, J. T. M.
245 $aEffect of natural mating or laparoscopic artificial insemination in superovulated Santa Inês ewes on superovulatory response, fertility and embryo viability.$h[electronic resource]
260 $c2016
520 $aAbstract: This study evaluated the effect of two mating methods (GNM: natural mating or GAI: laparoscopic artificial insemination) on superovulatory response, fertility and embryo yield in superovulated ewes. Fifteen non-pregnant Santa Inês ewes were superovulated and either mated by GNM or GAI in a crossover design. Oestrus was synchronised using intravaginal progestagen sponges for 6 days and on Day 5, 300 IU eCG and 0.0375 mg d-cloprostenol were given. Twelve hours after sponge removal, 0.025 mg gonadotropin-releasing hormone was administered. Superovulation started 48 h after gonadotropin-releasing hormone treatment, using 5 IU/kg follicle-stimulating hormone (pFSH). At the first pFSH dose, new sponges were inserted. At the fifth dose, 0.0375 mg cloprostenol was administered and the sponges were removed. The GNM was mated with rams every 12 h, until the end of oestrus. The ewes of GAI were laparoscopic inseminated with frozen?thawed semen 36 and 48 h after sponge removal. Ultrasonography was performed every 24 h from the beginning of oestrus synchronisation treatment and every 12 h from the second sponge removal to 2 days after the last pFSH dose. Six to seven days after mating, the number of corpora lutea (CL) was evaluated by laparoscopy and the females with >4CL were subjected to embryo collection. The interval from sponge removal to ovulation was shorter (P < 0.05) in the GNM. The overall superovulatory response was 63.3% (19/30), with 60.0% and 66.7% in GNM and GAI, respectively (P > 0.05). The number of recovered structures (6.4 ± 2.4 vs 4.5 ± 3.0), recovery rate (74.0 ± 16.0 vs 52.3 ± 26.5%), number of transferable embryos (3.0 ± 2.9 vs 3.6 ± 2.0) and viability rate (47.2 ± 45.3 vs 77.4 ± 37.1%) did not differ between GAI and GNM (P > 0.05). However, the GAI group showed a higher (P < 0.05) number of unfertilised oocytes (3.1 ± 3.1) and a higher non-fertilisation rate (47.1 ± 45.3%) than the GNM (0.9 ± 2.1 and 11.5 ± 21.5%). The mating method did not affect the superovulatory response, and production of viable embryos although the non-fertilisation rate has been inferior for the AI group.
650 $aArtificial insemination
650 $aEmbryo transfer
650 $aSheep
650 $aSuperovulation
650 $aInseminação Artificial
650 $aOvino
650 $aReprodução Animal
650 $aSuperovulação
650 $aTransferência de Embrião
653 $aEstrus synchronisation
653 $aMOET
653 $aOestrus synchronization
653 $aRaça Santa Inês
700 1 $aFONSECA, J. F. da
700 1 $aBALARO, M. F. A.
700 1 $aESTEVES, L. V.
700 1 $aASCOLI, F. O.
700 1 $aLEITE, R. C.
700 1 $aRIBEIRO, A. C. S.
700 1 $aDELGADO, K. F.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aTORRES FILHO, R. A.
700 1 $aBRANDÃO, F. Z.
773 $tAnimal Production Science$gv. 56, n. 9, p. 1463-1468, 2016.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
24/04/2014 |
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Data da última atualização: |
28/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SANTOS, P. M.; CORRÊA, C.; FORATO, L. A.; TULLIO, R. R.; CRUZ, G. M. D.; COLNAGO, L. A. |
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Afiliação: |
POLIANA M. SANTOS, INSTITUTO DE QUÍMICA- SÃO CARLOS, SP; CÁTIA C. CORRÊA, INSTITUTO DE QUIMÍCA USP/SÃO CARLOS, SP; LUCIMARA APARECIDA FORATO, CNPDIA; RYMER RAMIZ TULLIO, CPPSE; GERALDO MARIA DA CRUZ, CPPSE; LUIZ ALBERTO COLNAGO, CNPDIA. |
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Título: |
A fast and non-destructive method to discriminate beef samples using TD-NMR. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Food Control, v. 38, p.204-208, apr. 2014. |
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ISSN: |
0956-7135 |
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DOI: |
https://doi.org/10.1016/j.foodcont.2013.10.026 |
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Idioma: |
Inglês |
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Conteúdo: |
Carr-Purcell-Meiboom-Gill (CPMG) and Continuous Wave Free Precision (CWFP) pulse sequences were used to obtain time-domain 1H NuclearMagnetic Resonance (TD-NMR) data of beef samples. A total of 99 beef samples from different bull race (Angus, Bonsmara and Canchim) and sex (heifers and steer) were used in this study. The CPMG and CWFP data sets were processed using univariate and multivariate analysis in order to develop classification models to discriminate the samples according to the animal sex and bull race. A paired Student?s t test indicated that the univariate models obtained with CWFP data set (using Mz/Mo) had superior performance than those obtained with CPMG (using T2 values). Multivariate models showed similar predictability for both pulse sequences, with an average of correct classification higher than 80% for most of models developed. In general, the results showed that the TD-NMR spectroscopy when combined with univariate and multivariate analysis could provide a valuable tool for tracing the sex and bull race of beef samples, providing a reliable identification in a rapid, relatively cheap and non-invasive way, even in packages. |
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Palavras-Chave: |
CPMG; CWFP; TD NMR. |
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Thesaurus NAL: |
beef. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01856naa a2200253 a 4500
001 1985050
005 2023-03-28
008 2014 bl uuuu u00u1 u #d
022 $a0956-7135
024 7 $ahttps://doi.org/10.1016/j.foodcont.2013.10.026$2DOI
100 1 $aSANTOS, P. M.
245 $aA fast and non-destructive method to discriminate beef samples using TD-NMR.$h[electronic resource]
260 $c2014
520 $aCarr-Purcell-Meiboom-Gill (CPMG) and Continuous Wave Free Precision (CWFP) pulse sequences were used to obtain time-domain 1H NuclearMagnetic Resonance (TD-NMR) data of beef samples. A total of 99 beef samples from different bull race (Angus, Bonsmara and Canchim) and sex (heifers and steer) were used in this study. The CPMG and CWFP data sets were processed using univariate and multivariate analysis in order to develop classification models to discriminate the samples according to the animal sex and bull race. A paired Student?s t test indicated that the univariate models obtained with CWFP data set (using Mz/Mo) had superior performance than those obtained with CPMG (using T2 values). Multivariate models showed similar predictability for both pulse sequences, with an average of correct classification higher than 80% for most of models developed. In general, the results showed that the TD-NMR spectroscopy when combined with univariate and multivariate analysis could provide a valuable tool for tracing the sex and bull race of beef samples, providing a reliable identification in a rapid, relatively cheap and non-invasive way, even in packages.
650 $abeef
653 $aCPMG
653 $aCWFP
653 $aTD NMR
700 1 $aCORRÊA, C.
700 1 $aFORATO, L. A.
700 1 $aTULLIO, R. R.
700 1 $aCRUZ, G. M. D.
700 1 $aCOLNAGO, L. A.
773 $tFood Control$gv. 38, p.204-208, apr. 2014.
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Embrapa Pecuária Sudeste (CPPSE) |
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