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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
16/12/2016 |
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Data da última atualização: |
16/12/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KICH, J. D.; COSTA, E. de F.; TRIQUES, N. J.; NOGUEIRA, M. G.; DALLA COSTA, O. A.; COLDEBELLA, A.; CARDOSO, M. |
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Afiliação: |
JALUSA DEON KICH, CNPSA; EDUARDO D FREITAS COSTA, UFRGS/Faculdade de veterinária; NELISE JULIANE TRIQUES, UFRGS/Faculdade de veterinária; MARIANA GOMES NOGUEIRA, UFRGS/Faculdade de veterinária; OSMAR ANTONIO DALLA COSTA, CNPSA; ARLEI COLDEBELLA, CNPSA; MARISA CARDOSO, UFRGS/Faculdade de veterinária. |
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Título: |
Assessment of different cut-off values of the ELISA-Typhimurium for the discrimination of swine herds with Salmonella isolation. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Semina. Ciências Agrárias, Londrina, v. 37, n. 5, p. 3107-3114, 2016. |
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DOI: |
10.5433/1679-0359.2016v37n5p3107 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Salmonella control programs require the classification of positive herds according with the prevalence of infected pigs. This must be based on diagnostic methods that are reliable, accurate and cost effective. Since serological methods fulfill most of these prerequisites, many countries have adopted indirect ELISA tests to discriminate herds, based on their seroprevalence. In view of that, an in-house indirect ELISA test (ELISA-Typhimurium) previously developed proved to be able to detect sera antibodies against the most prevalent serovars of Salmonella found in Brazilian pig farms. This study aimed to assess different cut-off values of the ELISA-Typhimurium in terms of their correlation with Salmonella isolation results at the herd level. Blood and mesenteric lymph node or rectal swabs were taken from 56 slaughter and 14 grower batches from different farms. Serum was tested using the ELISA-Typhimurium at different cut-off points (OD: 6.87%-40%). A total of 55 slaughter batches had at least one Salmonellapositive sample, and the within-herd prevalence was 64.9% (SE±24.8). All 14-grower batches were negative on Salmonella isolation. The logistic regression demonstrated that at 20% OD the ELISATyphimurium test is the best model to predict Salmonella isolation frequency at the herd level. At this cut-off point, the test may be a helpful tool for discriminating Salmonella infected herds. Key words: Cut-off. ELISA. Salmonella detection. Serology. Swine. Resumo: Os programas de controle de Salmonella requerem a classificação de rebanhos de acordo com a prevalência de suínos infectados. Isto deve ser baseado em métodos de diagnóstico confiáveis, acurados e econômicos. Como os métodos sorológicos atendem estes pré-requisitos, muitos países têm adotado o teste de ELISA indireto na discriminação dos rebanhos, baseado na soroprevalência. Em vista disto, um teste de ELISA indireto (ELISA-Typhimurium) previamente desenvolvido demonstrou ser capaz de detectar anticorpos contra os sorovares de Salmonella mais prevalentes em granjas de suínos no Brasil. O presente estudo objetivou testar diferentes pontos de corte do teste ELISA-Typhimurium em termos de sua correlação com os resultados de isolamento de Salmonella na granja. Sangue e linfonodos mesentéricos ou suabes fecais foram colhidos de suínos pertencentes a 56 lotes de abate e 14 lotes de crescimento em diferentes granjas. O soro foi testado no ELISA-Typhimurium em diferentes pontos de corte (DO: 6,87%-40%). Um total de 55 lotes de abate testados apresentaram pelo menos uma amostra com isolamento de Salmonella, e a prevalência intra-lote foi de 64,9% (EP±24,8). Todos os lotes de crescimento foram negativos no isolamento de Salmonella. A regressão logística demostrou que a DO20% é o melhor modelo para predizer a frequência de isolamento de Salmonella em lotes de suínos. Nesse ponto de corte, o teste poderá ser uma ferramenta útil para discriminar lotes suínos infectados por Salmonella. MenosAbstract: Salmonella control programs require the classification of positive herds according with the prevalence of infected pigs. This must be based on diagnostic methods that are reliable, accurate and cost effective. Since serological methods fulfill most of these prerequisites, many countries have adopted indirect ELISA tests to discriminate herds, based on their seroprevalence. In view of that, an in-house indirect ELISA test (ELISA-Typhimurium) previously developed proved to be able to detect sera antibodies against the most prevalent serovars of Salmonella found in Brazilian pig farms. This study aimed to assess different cut-off values of the ELISA-Typhimurium in terms of their correlation with Salmonella isolation results at the herd level. Blood and mesenteric lymph node or rectal swabs were taken from 56 slaughter and 14 grower batches from different farms. Serum was tested using the ELISA-Typhimurium at different cut-off points (OD: 6.87%-40%). A total of 55 slaughter batches had at least one Salmonellapositive sample, and the within-herd prevalence was 64.9% (SE±24.8). All 14-grower batches were negative on Salmonella isolation. The logistic regression demonstrated that at 20% OD the ELISATyphimurium test is the best model to predict Salmonella isolation frequency at the herd level. At this cut-off point, the test may be a helpful tool for discriminating Salmonella infected herds. Key words: Cut-off. ELISA. Salmonella detection. Serology. Swine. Resumo: Os progr... Mostrar Tudo |
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Thesagro: |
Doença animal; Sanidade Animal; Suíno. |
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Thesaurus Nal: |
Animal diseases; Animal health; Bacteriology; Swine; Veterinary medicine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03941naa a2200301 a 4500
001 2058842
005 2016-12-16
008 2016 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2016v37n5p3107$2DOI
100 1 $aKICH, J. D.
245 $aAssessment of different cut-off values of the ELISA-Typhimurium for the discrimination of swine herds with Salmonella isolation.$h[electronic resource]
260 $c2016
520 $aAbstract: Salmonella control programs require the classification of positive herds according with the prevalence of infected pigs. This must be based on diagnostic methods that are reliable, accurate and cost effective. Since serological methods fulfill most of these prerequisites, many countries have adopted indirect ELISA tests to discriminate herds, based on their seroprevalence. In view of that, an in-house indirect ELISA test (ELISA-Typhimurium) previously developed proved to be able to detect sera antibodies against the most prevalent serovars of Salmonella found in Brazilian pig farms. This study aimed to assess different cut-off values of the ELISA-Typhimurium in terms of their correlation with Salmonella isolation results at the herd level. Blood and mesenteric lymph node or rectal swabs were taken from 56 slaughter and 14 grower batches from different farms. Serum was tested using the ELISA-Typhimurium at different cut-off points (OD: 6.87%-40%). A total of 55 slaughter batches had at least one Salmonellapositive sample, and the within-herd prevalence was 64.9% (SE±24.8). All 14-grower batches were negative on Salmonella isolation. The logistic regression demonstrated that at 20% OD the ELISATyphimurium test is the best model to predict Salmonella isolation frequency at the herd level. At this cut-off point, the test may be a helpful tool for discriminating Salmonella infected herds. Key words: Cut-off. ELISA. Salmonella detection. Serology. Swine. Resumo: Os programas de controle de Salmonella requerem a classificação de rebanhos de acordo com a prevalência de suínos infectados. Isto deve ser baseado em métodos de diagnóstico confiáveis, acurados e econômicos. Como os métodos sorológicos atendem estes pré-requisitos, muitos países têm adotado o teste de ELISA indireto na discriminação dos rebanhos, baseado na soroprevalência. Em vista disto, um teste de ELISA indireto (ELISA-Typhimurium) previamente desenvolvido demonstrou ser capaz de detectar anticorpos contra os sorovares de Salmonella mais prevalentes em granjas de suínos no Brasil. O presente estudo objetivou testar diferentes pontos de corte do teste ELISA-Typhimurium em termos de sua correlação com os resultados de isolamento de Salmonella na granja. Sangue e linfonodos mesentéricos ou suabes fecais foram colhidos de suínos pertencentes a 56 lotes de abate e 14 lotes de crescimento em diferentes granjas. O soro foi testado no ELISA-Typhimurium em diferentes pontos de corte (DO: 6,87%-40%). Um total de 55 lotes de abate testados apresentaram pelo menos uma amostra com isolamento de Salmonella, e a prevalência intra-lote foi de 64,9% (EP±24,8). Todos os lotes de crescimento foram negativos no isolamento de Salmonella. A regressão logística demostrou que a DO20% é o melhor modelo para predizer a frequência de isolamento de Salmonella em lotes de suínos. Nesse ponto de corte, o teste poderá ser uma ferramenta útil para discriminar lotes suínos infectados por Salmonella.
650 $aAnimal diseases
650 $aAnimal health
650 $aBacteriology
650 $aSwine
650 $aVeterinary medicine
650 $aDoença animal
650 $aSanidade Animal
650 $aSuíno
700 1 $aCOSTA, E. de F.
700 1 $aTRIQUES, N. J.
700 1 $aNOGUEIRA, M. G.
700 1 $aDALLA COSTA, O. A.
700 1 $aCOLDEBELLA, A.
700 1 $aCARDOSO, M.
773 $tSemina. Ciências Agrárias, Londrina$gv. 37, n. 5, p. 3107-3114, 2016.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Tipo/Formato |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
20/06/2017 |
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Data da última atualização: |
09/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
TOSCANO, L. T.; SILVA, A. S.; TOSCANO, L. T.; TAVARES, R. L.; BIASOTO, A. C. T.; CAMARGO, A. C. de; SILVA, C. S. O. da; GONÇALVES, M. da C. R.; SHAHIDI, F. |
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Afiliação: |
LYDIANE TAVARES TOSCANO, Department of Nutrition, Federal University of Paraiba, João Pessoa, PB; ALEXANDRE SÉRGIO SILVA, Department of Physical Education, Federal University of Paraíba, João Pessoa, PB; LUCIANA TAVARES TOSCANO, Department of Nutrition, Federal University of Paraiba, João Pessoa, PB; RENATA LEITE TAVARES, Department of Nutrition, Federal University of Paraiba, João Pessoa, PB; ALINE TELLES BIASOTO MARQUES, CPATSA; ADRIANO COSTA DE CAMARGO, Department of Biochemistry, Memorial University of Newfoundland, St. John’s, Canada; CÁSSIA SURAMA OLIVEIRA DA SILVA, Department of Nutrition, Federal University of Paraiba, João Pessoa, PB; MARIA DA CONCEIÇÃO RODRIGUES GONÇALVES, Department of Nutrition, Federal University of Paraiba, João Pessoa, PB; FEREIDOON SHAHIDI, Department of Biochemistry, Memorial University of Newfoundland, St. John’s, Canada. |
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Título: |
Phenolics from purple grape juice increase serum antioxidant status and improve lipid profile and blood pressure in healthy adults under intense physical training. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Functional Foods, v. 33, p. 419-424, 2017. |
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DOI: |
10.1016/j.jff.2017.03.063 |
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Idioma: |
Inglês |
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Conteúdo: |
Phenolic compounds have generated considerable interest in the scientific community and the media. Much of this interest is due to the properties ascribed to several phenolic compounds for prevention of cancer, dyslipidemia, and obesity. Grape juice made from cultivars Isabel, Bordeaux and Concord, was characterized in this work. Subsequently, twenty-eight healthy adults were randomly assigned to a group that consumed grape juice (GJG = 15; 25?54 years old) and a group that consumed a contro beverage (CG = 13; 20?53 years old). The groups were evaluated for their nutritional status, blood pressure, and blood collection for analysis of total antioxidant capacity, nitrite, glucose and lipid profile, upon receiving supplementation (10 m/kg/day) for 28 days and reevaluated at the end. The results suggest that purple grape juice had a great potential as an antioxidant source in improving antioxidant status and cardiometabolic profile of healthy adults under intense physical exercise. |
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Palavras-Chave: |
Bebida funcional; Estado antioxidante de plasma; Functional beverage; Human trial; Plasma antioxidant status; Polifenóis Julgamento humano. |
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Thesagro: |
Composto fenólico; Flavonóide; Suco; Suco de Fruta; Uva; Vitis Vinifera. |
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Thesaurus NAL: |
Polyphenols. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02190naa a2200385 a 4500
001 2071124
005 2018-01-09
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jff.2017.03.063$2DOI
100 1 $aTOSCANO, L. T.
245 $aPhenolics from purple grape juice increase serum antioxidant status and improve lipid profile and blood pressure in healthy adults under intense physical training.$h[electronic resource]
260 $c2017
520 $aPhenolic compounds have generated considerable interest in the scientific community and the media. Much of this interest is due to the properties ascribed to several phenolic compounds for prevention of cancer, dyslipidemia, and obesity. Grape juice made from cultivars Isabel, Bordeaux and Concord, was characterized in this work. Subsequently, twenty-eight healthy adults were randomly assigned to a group that consumed grape juice (GJG = 15; 25?54 years old) and a group that consumed a contro beverage (CG = 13; 20?53 years old). The groups were evaluated for their nutritional status, blood pressure, and blood collection for analysis of total antioxidant capacity, nitrite, glucose and lipid profile, upon receiving supplementation (10 m/kg/day) for 28 days and reevaluated at the end. The results suggest that purple grape juice had a great potential as an antioxidant source in improving antioxidant status and cardiometabolic profile of healthy adults under intense physical exercise.
650 $aPolyphenols
650 $aComposto fenólico
650 $aFlavonóide
650 $aSuco
650 $aSuco de Fruta
650 $aUva
650 $aVitis Vinifera
653 $aBebida funcional
653 $aEstado antioxidante de plasma
653 $aFunctional beverage
653 $aHuman trial
653 $aPlasma antioxidant status
653 $aPolifenóis Julgamento humano
700 1 $aSILVA, A. S.
700 1 $aTOSCANO, L. T.
700 1 $aTAVARES, R. L.
700 1 $aBIASOTO, A. C. T.
700 1 $aCAMARGO, A. C. de
700 1 $aSILVA, C. S. O. da
700 1 $aGONÇALVES, M. da C. R.
700 1 $aSHAHIDI, F.
773 $tJournal of Functional Foods$gv. 33, p. 419-424, 2017.
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