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Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
13/06/2016 |
Data da última atualização: |
13/06/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ALMEIDA, F. de; MEDEIROS, M. I. C.; KICH, J. D.; FALCÃO, J. P. |
Afiliação: |
FERNANDA DE ALMEIDA, USP/Faculdade de Ciências farmacêuticas de Ribeirão Preto; MARTA INÊS CAZENTINI MEDEIROS, Instituto Adolfo Lutz; JALUSA DEON KICH, CNPSA; JULIANA PFRIMER FALCÃO, USP/Faculdade de Ciências Farmacêuticas de Ribeirão Preto. |
Título: |
Virulence-associated genes, antimicrobial resistance and molecular typing of salmonella typhimurium strains isolated from swine from 2000 to 2012 in Brazil. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Journal of Applied Microbiology, v. 120, n. 6, p. 1677-1690, 2016. |
DOI: |
10.1111/jam.13110 |
Idioma: |
Inglês |
Conteúdo: |
Abstract Aims: The aims of this study were to assess the pathogenic potential, antimicrobial resistance and genotypic diversity of Salmonella Typhimurium strains isolated in Brazil from swine (22) and the surrounding swine environment (5) from 2000 to 2012 and compare them to the profiles of 43 human strains isolated from 1983 to 2010, which had been previously studied. Methods and Results: The presence of 12 SPI-1, SPI-2 and plasmid genes was assessed by PCR, the antimicrobial susceptibility to 13 antimicrobials was determined by the disc diffusion assay and genotyping was performed using pulsed-field gel electrophoresis (PFGE), multiple-locus variable-number of tandem repeats analysis (MLVA) and ERIC-PCR. More than 778% of the swine strains carried 10 or more of the virulence markers. Ten (37%) strains isolated from swine were multi-drug resistant (MDR). All the molecular typing techniques grouped the strains in two main clusters. Some strains isolated from swine and humans were allocated together in the PFGE-B2, MLVA-A1, MLVA-B and ERIC-A1 clusters. Conclusions: The genotyping results suggest that some strains isolated from swine and humans may descend from a common subtype and may indicate a possible risk of MDR S. Typhimurium with high frequency of virulence genes isolated from swine to contaminate humans in Brazil. Significance and Impact of the Study: This study provided new information about the pathogenic potential, antimicrobial resistance and genotypic diversity of S. Typhimurium isolates from swine origin in Brazil, the fourth largest producer of pigs worldwide. MenosAbstract Aims: The aims of this study were to assess the pathogenic potential, antimicrobial resistance and genotypic diversity of Salmonella Typhimurium strains isolated in Brazil from swine (22) and the surrounding swine environment (5) from 2000 to 2012 and compare them to the profiles of 43 human strains isolated from 1983 to 2010, which had been previously studied. Methods and Results: The presence of 12 SPI-1, SPI-2 and plasmid genes was assessed by PCR, the antimicrobial susceptibility to 13 antimicrobials was determined by the disc diffusion assay and genotyping was performed using pulsed-field gel electrophoresis (PFGE), multiple-locus variable-number of tandem repeats analysis (MLVA) and ERIC-PCR. More than 778% of the swine strains carried 10 or more of the virulence markers. Ten (37%) strains isolated from swine were multi-drug resistant (MDR). All the molecular typing techniques grouped the strains in two main clusters. Some strains isolated from swine and humans were allocated together in the PFGE-B2, MLVA-A1, MLVA-B and ERIC-A1 clusters. Conclusions: The genotyping results suggest that some strains isolated from swine and humans may descend from a common subtype and may indicate a possible risk of MDR S. Typhimurium with high frequency of virulence genes isolated from swine to contaminate humans in Brazil. Significance and Impact of the Study: This study provided new information about the pathogenic potential, antimicrobial resistance and genotypic diversity o... Mostrar Tudo |
Thesagro: |
Bactéria; Microbiologia; Patogenicidade; Produção animal; Salmonella Typhimurium; Suíno. |
Thesaurus Nal: |
Swine production; Virulence. |
Categoria do assunto: |
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Marc: |
LEADER 02445naa a2200265 a 4500 001 2046836 005 2016-06-13 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1111/jam.13110$2DOI 100 1 $aALMEIDA, F. de 245 $aVirulence-associated genes, antimicrobial resistance and molecular typing of salmonella typhimurium strains isolated from swine from 2000 to 2012 in Brazil.$h[electronic resource] 260 $c2016 520 $aAbstract Aims: The aims of this study were to assess the pathogenic potential, antimicrobial resistance and genotypic diversity of Salmonella Typhimurium strains isolated in Brazil from swine (22) and the surrounding swine environment (5) from 2000 to 2012 and compare them to the profiles of 43 human strains isolated from 1983 to 2010, which had been previously studied. Methods and Results: The presence of 12 SPI-1, SPI-2 and plasmid genes was assessed by PCR, the antimicrobial susceptibility to 13 antimicrobials was determined by the disc diffusion assay and genotyping was performed using pulsed-field gel electrophoresis (PFGE), multiple-locus variable-number of tandem repeats analysis (MLVA) and ERIC-PCR. More than 778% of the swine strains carried 10 or more of the virulence markers. Ten (37%) strains isolated from swine were multi-drug resistant (MDR). All the molecular typing techniques grouped the strains in two main clusters. Some strains isolated from swine and humans were allocated together in the PFGE-B2, MLVA-A1, MLVA-B and ERIC-A1 clusters. Conclusions: The genotyping results suggest that some strains isolated from swine and humans may descend from a common subtype and may indicate a possible risk of MDR S. Typhimurium with high frequency of virulence genes isolated from swine to contaminate humans in Brazil. Significance and Impact of the Study: This study provided new information about the pathogenic potential, antimicrobial resistance and genotypic diversity of S. Typhimurium isolates from swine origin in Brazil, the fourth largest producer of pigs worldwide. 650 $aSwine production 650 $aVirulence 650 $aBactéria 650 $aMicrobiologia 650 $aPatogenicidade 650 $aProdução animal 650 $aSalmonella Typhimurium 650 $aSuíno 700 1 $aMEDEIROS, M. I. C. 700 1 $aKICH, J. D. 700 1 $aFALCÃO, J. P. 773 $tJournal of Applied Microbiology$gv. 120, n. 6, p. 1677-1690, 2016.
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Embrapa Suínos e Aves (CNPSA) |
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Registros recuperados : 49 | |
5. | | ARVOR, D.; MEIRELLES, M. S. P.; DUBREUIL, V.; BEGUÈ, A.; SHIMABUKURO, Y. E. Analyzing the agricultural transition in Mato Grosso, Brazil, using satellite-derived indices. Applied Geography, v. 32, p. 702-713, 2011.Biblioteca(s): Embrapa Solos. |
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8. | | ADAMI, M.; FREITAS, R. M. de; PADOVANI, C. R.; SHIMABUKURO, Y. E.; MOREIRA, M. A. Estudo da dinâmica espaço-temporal do bioma Pantanal por meio de imagens MODIS. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 10, p. 1371-1378, out. 2008. Título em inglês: Spatial-temporal analysis of MODIS image applied to dynamic of Pantanal biome.Biblioteca(s): Embrapa Unidades Centrais. |
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9. | | WALKER, R.; DeFRIES, R.; VERA-DIAZ, M. del C.; SHIMABUKURO, Y.; VENTURIERI, A. The expansion of intensive agriculture and ranching in brazilian Amazonia. In: KELLER, M.; BUSTAMANTE, M.; GASH, J.; DIAS, P. S. (Ed.). Amazonia and global change. Washington, DC: American Geophysical Union, 2009. p. 61-81. (Geophysical monograph series, 186).Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Amazônia Oriental. |
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11. | | TRABAQUINI, K.; SILVA, G. B. S. da; FORMAGGIO, A. R.; SHIMABUKURO, Y. E.; GALVÃO, L. S. Dynamics and distribution of anthropic occupation in the Cerrado of Mato Grosso in the period from 1990 to 2008. Geografia, Rio Claro, v. 38, n. 2, p. 209-224, maio/ago. 2013. p. 209-224Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Territorial. |
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12. | | SILVA, G. B. S. da; FORMAGGIO, A. R.; SHIMABUKURO, Y. E.; ADAMI, M.; SANO, E. E. Discriminação da cobertura vegetal do Cerrado matogrossense por meio de imagens MODIS. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 2, p. 186-194, fev. 2010 Título em inglês: Discrimination of Cerrado vegetation cover in the state of Mato Grosso using MODIS images.Biblioteca(s): Embrapa Unidades Centrais. |
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18. | | PADOVANI, C. R.; SHIMABUKURO, Y. E.; FREITAS, R. M.; ADAMI, M.; VETTORAZZI, C. A. Spatial analysis of Pantanal wetland flood dynamics determined from modis images: a case study . In: INTECOL INTERNATIONAL WETLANDS CONFERENCE, 8., Cuiabá, 2008. Big wetlands, big concerns: abstracts. [Sl.: s.n], 2008. p.160Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pantanal. |
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Registros recuperados : 49 | |
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