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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/03/2016 |
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Data da última atualização: |
25/04/2024 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
AQUINO, S. O. de; CARNEIRO, F. de A.; RÊGO, E. C. S.; ALVES, G. S. C.; MARRACCINI. P.; ANDRADE, A. C. |
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Afiliação: |
S. O. DE AQUINO, UFLA; F. DE A. CARNEIRO, UCB; E. C. S. RÊGO; G. S. C. ALVES, UFLA; P. MARRACCINI, CIRAD UMR AGAP; ALAN CARVALHO ANDRADE, CENARGEN. |
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Título: |
Functional analysis of CcDREB1D promoter region from haplotypes of Coffea canephora through genetic transformation of Nicotiana tabacum. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia, Colombia. [Proceedings...]. [Armenia]: ASIC, 2015. p. 42-46. |
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Idioma: |
Inglês |
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Conteúdo: |
In many higher plants, DREB genes were shown to be involved in the transduction pathways of abiotic stress. Previous results showed that CcDREB1D gene expression increased under drought stress in leaves of drought-tolerant clone 14 of Coffea canephora Conilon but not in those of the drought-susceptible clone 22. By sequencing the DREB1D promoter regions of these two clones, several nucleic polymorphisms (?single nucleotide polymorphism? [SNP] and insertion/deletion [INDELs]) were found. In order to know if these polymorphisms could explain the differences of DREB1D gene expression observed between the clones 14 and 22, 5' deletions of several alleles of the CcDREB1D promoter regions were made and cloned independently into the binary vector pBI101 in order to analyze their ability to control the expression of the uidA reporter gene in transgenic tobacco (Nicotiana tabacum) plants. This work shows preliminary results of DREB1D promoter constructs analyzed in leaves of N. tabacum. |
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Palavras-Chave: |
Coffee. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01675nam a2200181 a 4500
001 2041748
005 2024-04-25
008 2015 bl uuuu u00u1 u #d
100 1 $aAQUINO, S. O. de
245 $aFunctional analysis of CcDREB1D promoter region from haplotypes of Coffea canephora through genetic transformation of Nicotiana tabacum.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia, Colombia. [Proceedings...]. [Armenia]: ASIC, 2015. p. 42-46.$c2015
520 $aIn many higher plants, DREB genes were shown to be involved in the transduction pathways of abiotic stress. Previous results showed that CcDREB1D gene expression increased under drought stress in leaves of drought-tolerant clone 14 of Coffea canephora Conilon but not in those of the drought-susceptible clone 22. By sequencing the DREB1D promoter regions of these two clones, several nucleic polymorphisms (?single nucleotide polymorphism? [SNP] and insertion/deletion [INDELs]) were found. In order to know if these polymorphisms could explain the differences of DREB1D gene expression observed between the clones 14 and 22, 5' deletions of several alleles of the CcDREB1D promoter regions were made and cloned independently into the binary vector pBI101 in order to analyze their ability to control the expression of the uidA reporter gene in transgenic tobacco (Nicotiana tabacum) plants. This work shows preliminary results of DREB1D promoter constructs analyzed in leaves of N. tabacum.
653 $aCoffee
700 1 $aCARNEIRO, F. de A.
700 1 $aRÊGO, E. C. S.
700 1 $aALVES, G. S. C.
700 1 $aMARRACCINI. P.
700 1 $aANDRADE, A. C.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/03/2017 |
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Data da última atualização: |
29/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
BALZON, T. A.; LUIS, Z. G.; PEREIRA, J. E. S. |
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Afiliação: |
Talita Aparecida Balzon; Zanderluce Gomes Luis, UnB; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
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Título: |
New approaches to improve the efficiency of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.) from mature zygotic embryos. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In Vitro Cellular e Developmental Biology, v. 49, p. 41-50, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
We developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embryos had a haustorium, a plumule, and the first and second foliar sheaths. In differentiated embryos, the radicular protrusion was not apparent because it generally does not appear until after the first true leaves emerge. MenosWe developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embr... Mostrar Tudo |
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Palavras-Chave: |
Zygotic embryo. |
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Thesaurus NAL: |
morphogenesis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180927/1/Balzon2013-Article-NewApproachesToImproveTheEffic.pdf
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Marc: |
LEADER 02287naa a2200169 a 4500
001 2067490
005 2023-03-29
008 2013 bl uuuu u00u1 u #d
100 1 $aBALZON, T. A.
245 $aNew approaches to improve the efficiency of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.) from mature zygotic embryos.$h[electronic resource]
260 $c2013
520 $aWe developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embryos had a haustorium, a plumule, and the first and second foliar sheaths. In differentiated embryos, the radicular protrusion was not apparent because it generally does not appear until after the first true leaves emerge.
650 $amorphogenesis
653 $aZygotic embryo
700 1 $aLUIS, Z. G.
700 1 $aPEREIRA, J. E. S.
773 $tIn Vitro Cellular e Developmental Biology$gv. 49, p. 41-50, 2013.
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