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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amapá. Para informações adicionais entre em contato com cpafap.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
11/12/2014 |
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Data da última atualização: |
05/10/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BIANCHI, M. B.; JERÔNIMO, G. T.; PÁDUA, S. B.; SATAKE, F.; ISHIKAWA, M. M.; TAVARES-DIAS, M.; MARTINS, M. L. |
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Afiliação: |
MICAEL B. BIANCHI, UFSC; GABRIELA T. JERÔNIMO; SANTIAGO B. PÁDUA; FABIANA SATAKE; MÁRCIA M. ISHIKAWA; MARCOS TAVARES DIAS, CPAF-AP; MAURÍCIO L. MARTINS. |
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Título: |
The hematological profile of farmed Sorubim lima: reference intervals, cell morphology and cytochemistry. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Veterinarski Arhiv, Zagreb, v. 84, n. 6, p. 677-690, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Hematology implies rapid and practical analysis to assist the diagnosis of fish homeostatic imbalance. This study determined the blood reference intervals in an important native South American catfish farmed in the Brazilian Pantanal wetland, Sorubim lima (Siluriformes, Pimelodidae), and describes the morphological and cytochemical characteristics of the cells. A total of 92 fish reared in a fish farm were examined for reference intervals 25 - 75% of total plasmatic protein, hemoglobin, red blood cell count (RBC), hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC) and total thrombocyte and leukocyte count (WBC). Periodic acid Schiff (PAS) with salivaryamylase, bromophenol blue, Sudan black B and toluidine blue (metacromasy) were the cytochemical stains employed to signal glycogen, proteins, lipids and nuclei in thrombocytes and leucocytes. In the blood smears, monocytes, lymphocytes, neutrophils, basophils, eosinophils, PAS - positive granular lymphocytes (LG-PAS) and thrombocytes were observed. Siluriforme similar results were observed in neutrophils and eosinophils morphology. Compared to other pimelodidae RBC, hematocrit and MCV showed the lowest index variation. In contrast to other siluriformes fish, S. limashowed the concomitant presence of circulating eosinophils and basophils. |
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Palavras-Chave: |
Jurupensém. |
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Thesagro: |
Hematologia; Peixe de água doce. |
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Thesaurus Nal: |
freshwater fish; hematology. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02094naa a2200253 a 4500
001 2002344
005 2022-10-05
008 2014 bl uuuu u00u1 u #d
100 1 $aBIANCHI, M. B.
245 $aThe hematological profile of farmed Sorubim lima$breference intervals, cell morphology and cytochemistry.$h[electronic resource]
260 $c2014
520 $aHematology implies rapid and practical analysis to assist the diagnosis of fish homeostatic imbalance. This study determined the blood reference intervals in an important native South American catfish farmed in the Brazilian Pantanal wetland, Sorubim lima (Siluriformes, Pimelodidae), and describes the morphological and cytochemical characteristics of the cells. A total of 92 fish reared in a fish farm were examined for reference intervals 25 - 75% of total plasmatic protein, hemoglobin, red blood cell count (RBC), hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC) and total thrombocyte and leukocyte count (WBC). Periodic acid Schiff (PAS) with salivaryamylase, bromophenol blue, Sudan black B and toluidine blue (metacromasy) were the cytochemical stains employed to signal glycogen, proteins, lipids and nuclei in thrombocytes and leucocytes. In the blood smears, monocytes, lymphocytes, neutrophils, basophils, eosinophils, PAS - positive granular lymphocytes (LG-PAS) and thrombocytes were observed. Siluriforme similar results were observed in neutrophils and eosinophils morphology. Compared to other pimelodidae RBC, hematocrit and MCV showed the lowest index variation. In contrast to other siluriformes fish, S. limashowed the concomitant presence of circulating eosinophils and basophils.
650 $afreshwater fish
650 $ahematology
650 $aHematologia
650 $aPeixe de água doce
653 $aJurupensém
700 1 $aJERÔNIMO, G. T.
700 1 $aPÁDUA, S. B.
700 1 $aSATAKE, F.
700 1 $aISHIKAWA, M. M.
700 1 $aTAVARES-DIAS, M.
700 1 $aMARTINS, M. L.
773 $tVeterinarski Arhiv, Zagreb$gv. 84, n. 6, p. 677-690, 2014.
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Registro original: |
Embrapa Amapá (CPAF-AP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Unidades Centrais. |
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Data corrente: |
21/03/2018 |
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Data da última atualização: |
23/03/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
RODRIGUES, R. A.; MENESES, I. I. F. S.; JORGE, K. S. G.; SILVA, M. R.; SANTOS, L. R. dos; LILENBAUM, W.; ETGES, R. N.; ARAUJO, F. R. |
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Afiliação: |
Rudielle A. Rodrigues, Programa de Pós-Graduação em Ciências Veterinárias/Faculdade de Medicina Veterinária e Zootecnia - FAMEZ/Universidade Federal de Mato Grosso do Sul - UFMS; Ingrid I. F. S. Meneses, Programa de Pós-Graduação em Biotecnologia e Biodiversidade da Região Centro-Oeste/Universidade Federal de Mato Grosso do Sul - UFMS; Klaudia S. G. Jorge, Programa de Pós-Graduação em Ciências Veterinárias/Faculdade de Medicina Veterinária e Zootecnia - FAMEZ/Universidade Federal de Mato Grosso do Sul - UFMS; MARCIO ROBERTO SILVA, CNPGL; LENITA RAMIRES DOS SANTOS, CNPGC; Walter Lilenbaum, Laboratório de Bacteriologia Veterinária/Universidade Federal Fluminense - UFF; Rodrigo N. Etges, Secretaria da Agricultura/Pecuária e Irrigação; FLABIO RIBEIRO ARAUJO, CNPGC. |
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Título: |
False-negative reactions to the comparative intradermal tuberculin test for bovine tuberculosis. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 37, n. 12, p. 1380-1384, dezembro. 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Reações falso-negativas ao teste cervical comparativo para tuberculose bovina. |
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Conteúdo: |
According to the Brazilian National Program for the Control and Eradication of Animal Brucellosis and Tuberculosis (PNCEBT), the routine tests for the diagnosis of bovine tuberculosis in the country are the simple intradermal tuberculin test (SITT) of the Ministry of Agriculture, Livestock and Food Supply (MAPA), the caudal fold test and the comparative intradermal tuberculin test (CITT). The latter is also used as a confirmatory test. A group of 53 animals from three dairy herds in a focal area for bovine tuberculosis, that were submitted to depopulation in the state of Rio Grande do Sul, were submitted to the CITT. Tissues were cultured and the resulting colonies were confirmed by PCR and DNA sequencing. Among the 53 animals analyzed using the CITT, 32 (60.4%) were negative, 14 (26.4%) were positive and seven (13.2%) results were inconclusive. The CITT detected 11 of the 39 animals with culture-confirmed M. bovis infection as positive. Among the total of 14 uninfected animals based on cultures, the CBT detected eight as negative. Thus, the CITT demonstrated sensitivity of 28.2% and specificity of 57.1% for the population sampled. A total of 24/32 (75.0%) of the animals with negative CITT results were culture positive (confirmed by PCR) and were considered false negatives based on the CITT. The maintenance of these false-negative animals in herds has serious implications for the control of the disease, since they can be a source of infection. The addition of complementary tests could help identify such animals and increase the odds of diagnostic success. MenosAccording to the Brazilian National Program for the Control and Eradication of Animal Brucellosis and Tuberculosis (PNCEBT), the routine tests for the diagnosis of bovine tuberculosis in the country are the simple intradermal tuberculin test (SITT) of the Ministry of Agriculture, Livestock and Food Supply (MAPA), the caudal fold test and the comparative intradermal tuberculin test (CITT). The latter is also used as a confirmatory test. A group of 53 animals from three dairy herds in a focal area for bovine tuberculosis, that were submitted to depopulation in the state of Rio Grande do Sul, were submitted to the CITT. Tissues were cultured and the resulting colonies were confirmed by PCR and DNA sequencing. Among the 53 animals analyzed using the CITT, 32 (60.4%) were negative, 14 (26.4%) were positive and seven (13.2%) results were inconclusive. The CITT detected 11 of the 39 animals with culture-confirmed M. bovis infection as positive. Among the total of 14 uninfected animals based on cultures, the CBT detected eight as negative. Thus, the CITT demonstrated sensitivity of 28.2% and specificity of 57.1% for the population sampled. A total of 24/32 (75.0%) of the animals with negative CITT results were culture positive (confirmed by PCR) and were considered false negatives based on the CITT. The maintenance of these false-negative animals in herds has serious implications for the control of the disease, since they can be a source of infection. The addition of complementary t... Mostrar Tudo |
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Palavras-Chave: |
Bacteriological culture; Cultura bacteriológica; Reações falso-negativas; Teste Cervical Comparativo; Tuberculose bovina. |
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Thesagro: |
Mycobacterium Bovis. |
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Thesaurus NAL: |
Tuberculosis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174398/1/False-negative-reactions-to-the-comparative.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174263/1/False-negative-reactions-to-the-comparative.pdf
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Marc: |
LEADER 02625naa a2200301 a 4500
001 2089602
005 2018-03-23
008 2017 bl uuuu u00u1 u #d
100 1 $aRODRIGUES, R. A.
245 $aFalse-negative reactions to the comparative intradermal tuberculin test for bovine tuberculosis.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Reações falso-negativas ao teste cervical comparativo para tuberculose bovina.
520 $aAccording to the Brazilian National Program for the Control and Eradication of Animal Brucellosis and Tuberculosis (PNCEBT), the routine tests for the diagnosis of bovine tuberculosis in the country are the simple intradermal tuberculin test (SITT) of the Ministry of Agriculture, Livestock and Food Supply (MAPA), the caudal fold test and the comparative intradermal tuberculin test (CITT). The latter is also used as a confirmatory test. A group of 53 animals from three dairy herds in a focal area for bovine tuberculosis, that were submitted to depopulation in the state of Rio Grande do Sul, were submitted to the CITT. Tissues were cultured and the resulting colonies were confirmed by PCR and DNA sequencing. Among the 53 animals analyzed using the CITT, 32 (60.4%) were negative, 14 (26.4%) were positive and seven (13.2%) results were inconclusive. The CITT detected 11 of the 39 animals with culture-confirmed M. bovis infection as positive. Among the total of 14 uninfected animals based on cultures, the CBT detected eight as negative. Thus, the CITT demonstrated sensitivity of 28.2% and specificity of 57.1% for the population sampled. A total of 24/32 (75.0%) of the animals with negative CITT results were culture positive (confirmed by PCR) and were considered false negatives based on the CITT. The maintenance of these false-negative animals in herds has serious implications for the control of the disease, since they can be a source of infection. The addition of complementary tests could help identify such animals and increase the odds of diagnostic success.
650 $aTuberculosis
650 $aMycobacterium Bovis
653 $aBacteriological culture
653 $aCultura bacteriológica
653 $aReações falso-negativas
653 $aTeste Cervical Comparativo
653 $aTuberculose bovina
700 1 $aMENESES, I. I. F. S.
700 1 $aJORGE, K. S. G.
700 1 $aSILVA, M. R.
700 1 $aSANTOS, L. R. dos
700 1 $aLILENBAUM, W.
700 1 $aETGES, R. N.
700 1 $aARAUJO, F. R.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 37, n. 12, p. 1380-1384, dezembro. 2017.
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