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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
24/11/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROCHA, L. C.; BUSTAMANTE, F. de O.; SILVEIRA, R. A. D. S.; TORRES, G. A.; MITTELMANN, A.; TECHIO, V. H. |
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Afiliação: |
LAIANE CORSINI ROCHA, UFLA; FERNANDA OLIVEIRA BUSTAMANTE, UFLA; RAPHAELA APARECIDA DUARTE SILVEIRA, UFLA; GIOVANA AUGUSTA TORRES, UFLA; ANDREA MITTELMANN, CNPGL; VANIA HELENA TECHIO, UFLA. |
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Título: |
Functional repetitive sequences and fragile sites in chromosomes of Lolium perenne L. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Protoplasma, v. 252, n. 2, p. 451-460, 2015. |
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DOI: |
https://doi.org/10.1007/s00709-014-0690-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Lolium perenne is considered a high-quality forage widely used in temperate regions to meet the shortage of forage during the winter. In this species, some peculiarities related to cytogenetic aspects have already been described, as the variability in number and position of 45S ribosomal DNA (rDNA) sites and the expression of fragile sites, which require further studies to support the understanding of their causes and consequences. In this way, this study aimed to evaluate the relationship between the expression of fragile sites and functional repetitive sequences (rDNA and telomeric) in chromosomes of diploid and polyploid cultivars of L. perenne. The techniques of FISH, Ag-NOR and fluorescence banding were used to assess the distribution of sites of 45S rDNA, 5S, telomeric sequences, and the transcriptional activity of the 45S ribosomal genes and the distribution of AT- and/or GC-rich sequences in L. perenne, respectively. There was variability in the number and location of 45S rDNA sites, which was not observed for 5S rDNA sites. One of the genotypes showed two 45S rDNA sites on the same chromosome, located in different chromosome arms. Breaks and gaps were found in 45S rDNA sites in most metaphases evaluated for both cultivars. Telomeric sequences were not detected at the end of the chromosomal fragments corresponding to the location of breaks at 45S sites. Apparently, the transcriptional activity was modified in fragile sites. Variation in the number and size of nucleoli, nucleolar fusions and dissociations were observed. All CMA(+) bands were colocalized with the 45S sites. MenosLolium perenne is considered a high-quality forage widely used in temperate regions to meet the shortage of forage during the winter. In this species, some peculiarities related to cytogenetic aspects have already been described, as the variability in number and position of 45S ribosomal DNA (rDNA) sites and the expression of fragile sites, which require further studies to support the understanding of their causes and consequences. In this way, this study aimed to evaluate the relationship between the expression of fragile sites and functional repetitive sequences (rDNA and telomeric) in chromosomes of diploid and polyploid cultivars of L. perenne. The techniques of FISH, Ag-NOR and fluorescence banding were used to assess the distribution of sites of 45S rDNA, 5S, telomeric sequences, and the transcriptional activity of the 45S ribosomal genes and the distribution of AT- and/or GC-rich sequences in L. perenne, respectively. There was variability in the number and location of 45S rDNA sites, which was not observed for 5S rDNA sites. One of the genotypes showed two 45S rDNA sites on the same chromosome, located in different chromosome arms. Breaks and gaps were found in 45S rDNA sites in most metaphases evaluated for both cultivars. Telomeric sequences were not detected at the end of the chromosomal fragments corresponding to the location of breaks at 45S sites. Apparently, the transcriptional activity was modified in fragile sites. Variation in the number and size of nucleol... Mostrar Tudo |
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Palavras-Chave: |
45S rDNA; Chromosomal rearrangements; CMA banding; Fragile sites; Ryegrass; Synteny. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02385naa a2200265 a 4500
001 2000837
005 2024-02-05
008 2015 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00709-014-0690-4$2DOI
100 1 $aROCHA, L. C.
245 $aFunctional repetitive sequences and fragile sites in chromosomes of Lolium perenne L.$h[electronic resource]
260 $c2015
520 $aLolium perenne is considered a high-quality forage widely used in temperate regions to meet the shortage of forage during the winter. In this species, some peculiarities related to cytogenetic aspects have already been described, as the variability in number and position of 45S ribosomal DNA (rDNA) sites and the expression of fragile sites, which require further studies to support the understanding of their causes and consequences. In this way, this study aimed to evaluate the relationship between the expression of fragile sites and functional repetitive sequences (rDNA and telomeric) in chromosomes of diploid and polyploid cultivars of L. perenne. The techniques of FISH, Ag-NOR and fluorescence banding were used to assess the distribution of sites of 45S rDNA, 5S, telomeric sequences, and the transcriptional activity of the 45S ribosomal genes and the distribution of AT- and/or GC-rich sequences in L. perenne, respectively. There was variability in the number and location of 45S rDNA sites, which was not observed for 5S rDNA sites. One of the genotypes showed two 45S rDNA sites on the same chromosome, located in different chromosome arms. Breaks and gaps were found in 45S rDNA sites in most metaphases evaluated for both cultivars. Telomeric sequences were not detected at the end of the chromosomal fragments corresponding to the location of breaks at 45S sites. Apparently, the transcriptional activity was modified in fragile sites. Variation in the number and size of nucleoli, nucleolar fusions and dissociations were observed. All CMA(+) bands were colocalized with the 45S sites.
653 $a45S rDNA
653 $aChromosomal rearrangements
653 $aCMA banding
653 $aFragile sites
653 $aRyegrass
653 $aSynteny
700 1 $aBUSTAMANTE, F. de O.
700 1 $aSILVEIRA, R. A. D. S.
700 1 $aTORRES, G. A.
700 1 $aMITTELMANN, A.
700 1 $aTECHIO, V. H.
773 $tProtoplasma$gv. 252, n. 2, p. 451-460, 2015.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
25/02/2013 |
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Data da última atualização: |
28/02/2013 |
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Tipo da produção científica: |
Software |
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Autoria: |
MOURA, M. F.; MERCANTI, E.; PEIXOTO, B. M.; MARCACINI, R. M.; TAMADA, T.; LIMA, A. F.; SANTOS, F. F. dos; REZENDE, S. O.; HIGA, R. H. |
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Afiliação: |
MARIA FERNANDA MOURA, CNPTIA; ENZO MERCANTI, Estagiário/CNPTIA, IC/UNICAMP; BRUNO MALVEIRA PEIXOTO, Estagiário/CNPTIA, IC/Unicamp; RICARDO MARCONDES MARCACINI, ICMC/USP; THIAGO TAMADA, Estagiário/CNPTIA, IC/Unicamp; ANA FLÁVIA LIMA, Estagiário/CNPTIA, IMECC/Unicamp; FABIANO FERNADES DOS SANTOS, ICMC/USP; SOLANGE OLIVEIRA REZENDE, ICMC/USP; ROBERTO HIROSHI HIGA, CNPTIA. |
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Título: |
TaxEdit - Taxonomy Editor V 3.0. Versão 1.0. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Campinas: Embrapa Informática Agropecuária, 2012. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
Nesta terceira versão, a interface foi evoluída a fim de melhor acoplar as novas funcionalidades (vocabulário controlado, cardinalidade do vocabulário, importação de hierarquias por meio do xml padronizado para o projeto) e novos modos de rotulação - tanto hierárquicos como flats; além de manter controles de arquivos intermediários e de permitir construir pastas de análise de dados para cada coleção de textos, guardando as informações geradas em cada qual. |
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Palavras-Chave: |
Ambiente de software; Mineração de textos; Organização da informação; Text mining; Thesaurus. |
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Thesaurus NAL: |
Computer software. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01249nam a2200289 a 4500
001 1950830
005 2013-02-28
008 2012 bl uuuu u0uu1 u #d
100 1 $aMOURA, M. F.
245 $aTaxEdit - Taxonomy Editor V 3.0. Versão 1.0.
260 $aCampinas: Embrapa Informática Agropecuária$c2012
300 $c1 CD-ROM.
520 $aNesta terceira versão, a interface foi evoluída a fim de melhor acoplar as novas funcionalidades (vocabulário controlado, cardinalidade do vocabulário, importação de hierarquias por meio do xml padronizado para o projeto) e novos modos de rotulação - tanto hierárquicos como flats; além de manter controles de arquivos intermediários e de permitir construir pastas de análise de dados para cada coleção de textos, guardando as informações geradas em cada qual.
650 $aComputer software
653 $aAmbiente de software
653 $aMineração de textos
653 $aOrganização da informação
653 $aText mining
653 $aThesaurus
700 1 $aMERCANTI, E.
700 1 $aPEIXOTO, B. M.
700 1 $aMARCACINI, R. M.
700 1 $aTAMADA, T.
700 1 $aLIMA, A. F.
700 1 $aSANTOS, F. F. dos
700 1 $aREZENDE, S. O.
700 1 $aHIGA, R. H.
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Embrapa Agricultura Digital (CNPTIA) |
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