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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
29/05/2018 |
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Data da última atualização: |
30/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, D. R. de; TODO, A. H.; REGO, G. M.; CERUTTI, J. M.; CAVALHEIRO, A. J.; RANDO, D. G. G.; CERUTTI, S. M. |
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Afiliação: |
Daniela Rodrigues de Oliveira, Universidade Federal de São Paulo; Andréia Hatsue Todo, Universidade Federal de São Paulo; GIZELDA MAIA REGO, CNPF; Janete Maria Cerutti, Universidade Federal de São Paulo; Alberto José Cavalheiro, UNESP; Daniela Gonçales Galasse Rando, Universidade Federal de São Paulo; Suzete Maria Cerutti, Universidade Federal de São Paulo. |
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Título: |
Flavones-bound in benzodiazepine site on GABAA receptor: concomitant anxiolytic-like and cognitive-enhancing effects produced by Isovitexin and 6-C-glycoside-Diosmetin. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
European Journal of Pharmacology, v. 831, p. 77-86, July 2018. |
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DOI: |
10.1016/j.ejphar.2018.05.004 |
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Idioma: |
Inglês |
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Conteúdo: |
Increasing evidence suggests that flavones can modulate memory and anxiety-like behaviour. However, these therapeutic effects are inconsistent and induce of adverse effects, which have been associated with interactions at the Benzodiazepine (BZ)-binding site. To improve our understanding of flavone effects on memory and anxiety, we employed a plus-maze discriminative avoidance task. Furthermore, we evaluated the potential of the compounds in modulating GABAA receptors via BZ-binding site using molecular modelling studies. Adult male Wistar rats were treated 30 min before training session with Vicenin-2 (0.1 and 0.25 mg/kg), Vitexin (0.1 and 0.25 mg/kg), Isovitexin (0.1 and 0.25 mg/kg) and 0.1 mg/kg 6-C-glycoside-Diosmetin, vehicle and a GABAA receptor agonist. The analysis of the time spent in the non-aversive vs aversive enclosed arms during the test session and percentage of time in the open arms within the training session revealed that treatment with Isovitexin and 6-C-glycoside-Diosmetin had memory-enhancing and anxiolytic-like effects (P < 0.001). In contrast, treatment with a higher dose of Diazepam impaired short-and long-term memory when it alleviated anxiety level. Docking studies revealed that flavones docked in a very similar way to that observed to the Diazepam, except by a lack of interaction in residue α1His101 in the BZ-binding site on GABAA receptors, which may be related to memory-enhancing effect. The occurrence of the α1His101 interaction could justify the memory-impairing observed following Diazepam treatment. These findings provide the first evidence that Isovitexin and 6-C-glycoside-Diosmetin could exert their memory-enhancing and anxiolytic-like effects via GABAA receptor modulation, which likely occurs via their benzodiazepine-binding site. MenosIncreasing evidence suggests that flavones can modulate memory and anxiety-like behaviour. However, these therapeutic effects are inconsistent and induce of adverse effects, which have been associated with interactions at the Benzodiazepine (BZ)-binding site. To improve our understanding of flavone effects on memory and anxiety, we employed a plus-maze discriminative avoidance task. Furthermore, we evaluated the potential of the compounds in modulating GABAA receptors via BZ-binding site using molecular modelling studies. Adult male Wistar rats were treated 30 min before training session with Vicenin-2 (0.1 and 0.25 mg/kg), Vitexin (0.1 and 0.25 mg/kg), Isovitexin (0.1 and 0.25 mg/kg) and 0.1 mg/kg 6-C-glycoside-Diosmetin, vehicle and a GABAA receptor agonist. The analysis of the time spent in the non-aversive vs aversive enclosed arms during the test session and percentage of time in the open arms within the training session revealed that treatment with Isovitexin and 6-C-glycoside-Diosmetin had memory-enhancing and anxiolytic-like effects (P < 0.001). In contrast, treatment with a higher dose of Diazepam impaired short-and long-term memory when it alleviated anxiety level. Docking studies revealed that flavones docked in a very similar way to that observed to the Diazepam, except by a lack of interaction in residue α1His101 in the BZ-binding site on GABAA receptors, which may be related to memory-enhancing effect. The occurrence of the α1His101 interaction could ... Mostrar Tudo |
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Palavras-Chave: |
Ácido gama aminobutírico; Ansiedade; Anxiety and memory; Efeito ansiolítico; GABA; GABAAR; Memória; Neurotransmissor; PM-DAT; Receptor. |
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Thesagro: |
Erythrina Falcata; Farmacologia; Flavonóide. |
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Thesaurus Nal: |
Flavones. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02929naa a2200373 a 4500
001 2092046
005 2018-05-30
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.ejphar.2018.05.004$2DOI
100 1 $aOLIVEIRA, D. R. de
245 $aFlavones-bound in benzodiazepine site on GABAA receptor$bconcomitant anxiolytic-like and cognitive-enhancing effects produced by Isovitexin and 6-C-glycoside-Diosmetin.$h[electronic resource]
260 $c2018
520 $aIncreasing evidence suggests that flavones can modulate memory and anxiety-like behaviour. However, these therapeutic effects are inconsistent and induce of adverse effects, which have been associated with interactions at the Benzodiazepine (BZ)-binding site. To improve our understanding of flavone effects on memory and anxiety, we employed a plus-maze discriminative avoidance task. Furthermore, we evaluated the potential of the compounds in modulating GABAA receptors via BZ-binding site using molecular modelling studies. Adult male Wistar rats were treated 30 min before training session with Vicenin-2 (0.1 and 0.25 mg/kg), Vitexin (0.1 and 0.25 mg/kg), Isovitexin (0.1 and 0.25 mg/kg) and 0.1 mg/kg 6-C-glycoside-Diosmetin, vehicle and a GABAA receptor agonist. The analysis of the time spent in the non-aversive vs aversive enclosed arms during the test session and percentage of time in the open arms within the training session revealed that treatment with Isovitexin and 6-C-glycoside-Diosmetin had memory-enhancing and anxiolytic-like effects (P < 0.001). In contrast, treatment with a higher dose of Diazepam impaired short-and long-term memory when it alleviated anxiety level. Docking studies revealed that flavones docked in a very similar way to that observed to the Diazepam, except by a lack of interaction in residue α1His101 in the BZ-binding site on GABAA receptors, which may be related to memory-enhancing effect. The occurrence of the α1His101 interaction could justify the memory-impairing observed following Diazepam treatment. These findings provide the first evidence that Isovitexin and 6-C-glycoside-Diosmetin could exert their memory-enhancing and anxiolytic-like effects via GABAA receptor modulation, which likely occurs via their benzodiazepine-binding site.
650 $aFlavones
650 $aErythrina Falcata
650 $aFarmacologia
650 $aFlavonóide
653 $aÁcido gama aminobutírico
653 $aAnsiedade
653 $aAnxiety and memory
653 $aEfeito ansiolítico
653 $aGABA
653 $aGABAAR
653 $aMemória
653 $aNeurotransmissor
653 $aPM-DAT
653 $aReceptor
700 1 $aTODO, A. H.
700 1 $aREGO, G. M.
700 1 $aCERUTTI, J. M.
700 1 $aCAVALHEIRO, A. J.
700 1 $aRANDO, D. G. G.
700 1 $aCERUTTI, S. M.
773 $tEuropean Journal of Pharmacology$gv. 831, p. 77-86, July 2018.
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Registro original: |
Embrapa Florestas (CNPF) |
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Biblioteca |
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Origem |
Tipo/Formato |
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Cutter |
Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Rondônia. |
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Data corrente: |
11/01/1994 |
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Data da última atualização: |
23/09/2020 |
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Tipo da produção científica: |
Circular Técnica |
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Autoria: |
COSTA, N. de L.; GONCALVES, C. A.; OLIVEIRA, M. A. S.; OLIVEIRA, J. R. da C.; ROCHA, C. M. C. da. |
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Afiliação: |
NEWTON DE LUCENA COSTA, CPAF-RO; CARLOS ALBERTO GONÇALVES, CPAF-RO; MARIA ALICE SANTOS OLIVEIRA, CPAF-RO. |
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Título: |
Avaliação agronômica de germoplasmas de gramíneas forrageiras nos cerrados de Rondônia. |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
Porto Velho: EMBRAPA-UEPAE Porto Velho, 1988. |
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Páginas: |
8 p. |
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Série: |
(EMBRAPA-UEPAE Porto Velho. Comunicado técnico, 62). |
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Idioma: |
Português |
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Conteúdo: |
Avaliou-se novos germoplasmas de gramineas forrageiras mais promissoras para a formacao de pastagens melhoradas nos cerrados de Rondonia. O ensaio foi conduzido no campo experimental da UEPAE Porto Velho no muniicipio de Vilhena. Foram avaliados 45 ecotipos de gramineas forrageiras pertencentes aos seguintes generos: Panicum (29), Paspalum (3), Brachiaria (7) e Melinis (6), sem repeticao de campo. As parcelas mediam 2,5cm x 5,0m e foram adubadas por ocasiao do plantio com 60kg de N/ha, 60kg/ha de P2O5/ha e 86kg de K2O/ha, sob a forma de ureia, superfosfato triplo e cloreto de potassio, respectivamente. As gramineas mais promissoras foram: B. brizantha (CPAC-3132, CPAC-3099), p. Maximum (CPAC-3071, CPAC-3070, CPAC-3047, CPAC-3074, CPAC-3015, CPAC-3028, CPAC-3024, CPAC-3068, CPAC-3047, CPAC-3072, CPAC-3020 e CPAC-3021), B. humidicola, P. secans PCAP-12, P. coryphaeum FCAP-8 e M. minutiflora CPAC-3103. |
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Palavras-Chave: |
Amazônia Ocidental; Avaliacao; Avaliação agronômica; Brasil; Evaluation; Feed grasses; Forage grass; Forrageira; Pasture; Planta graminea forrageira; Rondônia; Valuation; Vilhena; Vilhena (RO); Western Amazon. |
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Thesagro: |
Aclimatação; Características Agronômicas; Cerrado; Comportamento de Variedade; Condição Ambiental; Fertilidade do Solo; Germoplasma; Gramínea; Gramínea Forrageira; Melhoramento; Pastagem; Produtividade; Rendimento. |
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Thesaurus NAL: |
Acclimation; Agronomic traits; Amazonia; Brazil; breeding; Cerrado soils; Crop yield; Environmental factors; forage; Forage grasses; germplasm; grasses; Pastures; Soil fertility; Variety trials. |
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Categoria do assunto: |
--
G Melhoramento Genético
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02764nam a2200697 a 4500
001 1698026
005 2020-09-23
008 1988 bl uuuu u0uu1 u #d
100 1 $aCOSTA, N. de L.
245 $aAvaliação agronômica de germoplasmas de gramíneas forrageiras nos cerrados de Rondônia.
260 $aPorto Velho: EMBRAPA-UEPAE Porto Velho$c1988
300 $a8 p.
490 $a(EMBRAPA-UEPAE Porto Velho. Comunicado técnico, 62).
520 $aAvaliou-se novos germoplasmas de gramineas forrageiras mais promissoras para a formacao de pastagens melhoradas nos cerrados de Rondonia. O ensaio foi conduzido no campo experimental da UEPAE Porto Velho no muniicipio de Vilhena. Foram avaliados 45 ecotipos de gramineas forrageiras pertencentes aos seguintes generos: Panicum (29), Paspalum (3), Brachiaria (7) e Melinis (6), sem repeticao de campo. As parcelas mediam 2,5cm x 5,0m e foram adubadas por ocasiao do plantio com 60kg de N/ha, 60kg/ha de P2O5/ha e 86kg de K2O/ha, sob a forma de ureia, superfosfato triplo e cloreto de potassio, respectivamente. As gramineas mais promissoras foram: B. brizantha (CPAC-3132, CPAC-3099), p. Maximum (CPAC-3071, CPAC-3070, CPAC-3047, CPAC-3074, CPAC-3015, CPAC-3028, CPAC-3024, CPAC-3068, CPAC-3047, CPAC-3072, CPAC-3020 e CPAC-3021), B. humidicola, P. secans PCAP-12, P. coryphaeum FCAP-8 e M. minutiflora CPAC-3103.
650 $aAcclimation
650 $aAgronomic traits
650 $aAmazonia
650 $aBrazil
650 $abreeding
650 $aCerrado soils
650 $aCrop yield
650 $aEnvironmental factors
650 $aforage
650 $aForage grasses
650 $agermplasm
650 $agrasses
650 $aPastures
650 $aSoil fertility
650 $aVariety trials
650 $aAclimatação
650 $aCaracterísticas Agronômicas
650 $aCerrado
650 $aComportamento de Variedade
650 $aCondição Ambiental
650 $aFertilidade do Solo
650 $aGermoplasma
650 $aGramínea
650 $aGramínea Forrageira
650 $aMelhoramento
650 $aPastagem
650 $aProdutividade
650 $aRendimento
653 $aAmazônia Ocidental
653 $aAvaliacao
653 $aAvaliação agronômica
653 $aBrasil
653 $aEvaluation
653 $aFeed grasses
653 $aForage grass
653 $aForrageira
653 $aPasture
653 $aPlanta graminea forrageira
653 $aRondônia
653 $aValuation
653 $aVilhena
653 $aVilhena (RO)
653 $aWestern Amazon
700 1 $aGONCALVES, C. A.
700 1 $aOLIVEIRA, M. A. S.
700 1 $aOLIVEIRA, J. R. da C.
700 1 $aROCHA, C. M. C. da
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