Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/12/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
GIRARDINI, L. K.; PAIM, D. S.; LOPES, G. V.; PELLEGRINI, D. C. P.; BRITO, M. A. V. P.; CARDOSO, M. |
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Afiliação: |
LILIAN K. GIRARDINI, Universidade do Oeste de Santa Catarina/FMV; DANIEL S. PAIM, UFRGS; GRACIELA V. LOPES, UFRGS; DEBORA C. P. PELLEGRINI, Universidade Federal do Pampa; MARIA APARECIDA V PAIVA E BRITO, CNPGL; MARISA CARDOSO, UFRGS. |
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Título: |
Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 10, p. 951-956, out. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
In intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ?4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by sporadic PFGE clusters, although some persistent clusters can arise over time. Both sporadic and persistent isolates were highly susceptible to antimicrobials. MenosIn intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ?4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by spor... Mostrar Tudo |
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Palavras-Chave: |
Grupo clonal; Mastistis. |
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Thesagro: |
Mamite. |
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Thesaurus NAL: |
Methicillin-resistant Staphylococcus aureus; Pulsed-field gel electrophoresis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/152571/1/Antimicrobial-resistance-profiles.pdf
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Marc: |
LEADER 02442naa a2200241 a 4500
001 2059656
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aGIRARDINI, L. K.
245 $aAntimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil.$h[electronic resource]
260 $c2016
520 $aIn intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ?4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by sporadic PFGE clusters, although some persistent clusters can arise over time. Both sporadic and persistent isolates were highly susceptible to antimicrobials.
650 $aMethicillin-resistant Staphylococcus aureus
650 $aPulsed-field gel electrophoresis
650 $aMamite
653 $aGrupo clonal
653 $aMastistis
700 1 $aPAIM, D. S.
700 1 $aLOPES, G. V.
700 1 $aPELLEGRINI, D. C. P.
700 1 $aBRITO, M. A. V. P.
700 1 $aCARDOSO, M.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 10, p. 951-956, out. 2016.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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