Data/hora: 19/06/2024 02:08:50

Biblioteca(s): | Embrapa Soja.
Data corrente: | 10/12/1992
Data da última atualização: | 09/04/2009
Autoria: | LAGUERRE, G.; MAZURIER, S. I.; AMARGER, N.
Afiliação: | Institut National de la Recherche Agronomique, Laboratoire de Microbiologie des Sols, 17 rue Sully, BV 1540, 21034 Dijon Cedex, France.
Título: | Plasmid profiles and restriction fragment length polymorphism of Rhizobium leguminosarum bv. viciae in field populations.
Ano de publicação: | 1992
Fonte/Imprenta: | FEMS Microbiology Ecology, v.101, p.17-26, 1992.
Idioma: | Inglês
Conteúdo: | 56 isolates of Rhizobium leguminosarum biovar viciae from one field were characterized by analysis of plasmid profile, total DNA restriction pattern and restriction fragment length polymorphism (RFLP) of 2 chromosomal regions and of symbiotic (Sym) plasmid. Different levels of similarity exist in patterns generated by the different techniques. At the level of partial similarity these techniques give comparable results for more than 80% of the isolates, with the exception of RFLP profiling with the Sym probe. Analysis at this level allows the grouping of the isolates that have most of their non-Sym genome similarity, the techniques are no more equivalent and provide complementary information on possible evolution of the different elements of the genome identified by each specific technique. The non-Sym plasmids defining classes were strongly associated with specific chromosomal backgrounds. In contrast, variations in Sym plasmids were not related with variations in the remaining genome. Host range towards chromosomes was variable among the Sym plasmids, which may reflect plasmid transfer between strains.
Palavras-Chave: | Diversidade genetica; DNA fingerprint; Genetic diversity; Length polymorphism; Methology; Metodologia; Perfil; Plasmid profiles; Restriction fragment; RFLP; Symbiotic plasmid.
Thesagro: | Plasmídeo; Rhizobium Leguminosarum.
Categoria do assunto: | --
Registro original: | Embrapa Soja (CNPSO)
Exemplares: | CNPSO - (13927-1) - ADD - SP - -- - 9916 -  - 9916 - DIS