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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão; Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
15/09/2022 |
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Data da última atualização: |
16/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, A. L.; FARIA, J. C. de; MOURA, M. da C.; ZAIDEM, A. L. de M.; PIZETTA, C. S. R.; FREITAS, E. de O.; COELHO, G. R. C.; SILVA, J. F. A. e; BARRIGOSSI, J. A. F.; HOFFMANN, L. V.; SOUZA, T. L. P. O. de; ARAGÃO, F. J. L.; PINHEIRO, P. V. |
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Afiliação: |
AMANDA LOPES FERREIRA, bolsista CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MATHEUS DA COSTA MOURA, UNIVERSIDADE FEDERAL DE GOIÁS; ANTÔNIA LOPES DE MENDONÇA ZAIDEM, UNIVERSIDADE FEDERAL DE GOIÁS; CAROLINA SENHORINHO RAMALHO PIZETTA; ELÍNEA DE OLIVEIRA FREITAS; GESIMARIA RIBEIRO COSTA COELHO, CNPAF; JOSE FRANCISCO ARRUDA E SILVA, CNPAF; JOSE ALEXANDRE F BARRIGOSSI, CNPAF; LUCIA VIEIRA HOFFMANN, CNPA; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen; PATRICIA VALLE PINHEIRO, CNPAF. |
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Título: |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 13, 984804, Aug. 2022. |
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ISSN: |
1664-462X |
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DOI: |
https://doi.org/10.3389/fpls.2022.984804 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases. MenosCommon bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays we... Mostrar Tudo |
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Palavras-Chave: |
Dry bean; Insect pest management; VATPase. |
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Thesagro: |
Bemisia Tabaci; Feijão; Mosca Branca; Phaseolus Vulgaris; Planta Transgênica. |
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Thesaurus Nal: |
Beans; RNA interference; Transgenic plants. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146488/1/fpls-984804.pdf
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Marc: |
LEADER 03529naa a2200421 a 4500
001 2146488
005 2022-09-16
008 2022 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $ahttps://doi.org/10.3389/fpls.2022.984804$2DOI
100 1 $aFERREIRA, A. L.
245 $aWhitefly-tolerant transgenic common bean (Phaseolus vulgaris) line.$h[electronic resource]
260 $c2022
520 $aCommon bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases.
650 $aBeans
650 $aRNA interference
650 $aTransgenic plants
650 $aBemisia Tabaci
650 $aFeijão
650 $aMosca Branca
650 $aPhaseolus Vulgaris
650 $aPlanta Transgênica
653 $aDry bean
653 $aInsect pest management
653 $aVATPase
700 1 $aFARIA, J. C. de
700 1 $aMOURA, M. da C.
700 1 $aZAIDEM, A. L. de M.
700 1 $aPIZETTA, C. S. R.
700 1 $aFREITAS, E. de O.
700 1 $aCOELHO, G. R. C.
700 1 $aSILVA, J. F. A. e
700 1 $aBARRIGOSSI, J. A. F.
700 1 $aHOFFMANN, L. V.
700 1 $aSOUZA, T. L. P. O. de
700 1 $aARAGÃO, F. J. L.
700 1 $aPINHEIRO, P. V.
773 $tFrontiers in Plant Science$gv. 13, 984804, Aug. 2022.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 8 | |
| 2. |  | CARVALHO, H. W. L. de; FARIA, L. C. de; WARWICK, D. R. N.; ALBUQUERQUE, M. M. de; DEL PELOSO, M. J.; MELO, L. C.; RODRIGUES, A. R. dos S.; OLIVEIRA, V. D. de; RIBEIRO, S. S.; SOUZA, E. M. de. Adaptabilidade e estabilidade de genótipos de feijoeiro comum no nordeste brasileiro no ano agrícola de 2005. Aracaju: Embrapa Tabuleiros Costeiros, 2006. 18 p. (Embrapa Tabuleiros Costeiros. Boletim de Pesquisa e Desenvolvimento, 16). Duplicado no sistema.| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Tabuleiros Costeiros. |
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| 7. | | BARBOSA, G. S.; RAMOS, A. de R.; MARCELLINO, L. H.; GANDER, E. S. Nim (Azadirachta indica) como candidato para controle biológico de fungos patogênicos ao cacau (Theobroma cacao). Fitopatologia Brasileira, Brasília, DF, v. 29, supl., p. S159, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | SANTOS, F. C. dos; ALBUQUERQUE FILHO, M. R. de; FERREIRA, G. B.; SILVA FILHO, J. L. da; CARVALHO, M. da C. S.; PEDROSA, M. B.; BARBOSA, C. A. da S.; SANTOS, J. B. dos. Pesquisas com correção do solo e adubação para o algodoeiro no Cerrado do Oeste baiano, sob diferentes sistemas de cultivo - Safra 2007/2008. Planaltina, DF: Embrapa Cerrados, 2008. (Embrapa Cerrados. Documentos, 232).| Biblioteca(s): Embrapa Algodão; Embrapa Cerrados. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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