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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
12/02/2014 |
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Data da última atualização: |
22/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SINSKI, I.; BOSCO, D. D.; PIEROZZI, N. I.; MAIA, J. D. G.; RITSCHEL, P. S.; QUECINI, V. |
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Afiliação: |
IRACI SINSKI, CNPUV; DANIELA DAL BOSCO, CNPUV; Neiva Izabel Pierozzi, IAC; JOAO DIMAS GARCIA MAIA, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; VERA MARIA QUECINI, CNPUV. |
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Título: |
Improving in vitro induction of autopolyploidy in grapevine seedless cultivars. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Euphytica, Wageningen, v. 196, n. 2, p. 299-311, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
The efficiency of in vitro polyploidization depends on several variables associated to the plant, the antimicrotubule agent and the interactions between them. In the present work, we have used response-surface methodology to determine the best operating conditions for plant recovery in polyploidization assays for shoot apices and somatic embryos of two seedless grape cultivars, employing colchicine and oryzalin. Explant type, tubulin-interfering compound and concentration were the critical factors determining plant recovery. Linear reduction in viable plant regeneration via organogenesis and somatic embryogenesis was obtained by increasing oryzalin concentrations and treatment time, whereas the effects of colchicine were better described by a quadratic design for both explants types. The conditions promoting higher rates of plant recovery were used in chromosome doubling experiments with oryzalin and colchicine for shoot apices and somatic embryos of ?Crimson seedless? and ?BRS Clara?. The established protocols allowed the recovery of non-chimerical autotetraploid plants at rates higher than 30 % for both cultivars. Stomata size parameters statistically correlate to the ploidy level of the regenerants and were effective for preliminary polyploidy screening. Autotetraploid lines of seedless grapes were incorporated into the Vitis germplasm bank for further agronomical evaluations. To our knowledge, this is the first report of in vitro oryzalin induced polyploidization of grapevine and of the use of mathematical modeling to optimize chromosome doubling in plants. MenosThe efficiency of in vitro polyploidization depends on several variables associated to the plant, the antimicrotubule agent and the interactions between them. In the present work, we have used response-surface methodology to determine the best operating conditions for plant recovery in polyploidization assays for shoot apices and somatic embryos of two seedless grape cultivars, employing colchicine and oryzalin. Explant type, tubulin-interfering compound and concentration were the critical factors determining plant recovery. Linear reduction in viable plant regeneration via organogenesis and somatic embryogenesis was obtained by increasing oryzalin concentrations and treatment time, whereas the effects of colchicine were better described by a quadratic design for both explants types. The conditions promoting higher rates of plant recovery were used in chromosome doubling experiments with oryzalin and colchicine for shoot apices and somatic embryos of ?Crimson seedless? and ?BRS Clara?. The established protocols allowed the recovery of non-chimerical autotetraploid plants at rates higher than 30 % for both cultivars. Stomata size parameters statistically correlate to the ploidy level of the regenerants and were effective for preliminary polyploidy screening. Autotetraploid lines of seedless grapes were incorporated into the Vitis germplasm bank for further agronomical evaluations. To our knowledge, this is the first report of in vitro oryzalin induced polyploidization of grap... Mostrar Tudo |
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Thesagro: |
Genética; Poliploidia; Uva; Viticultura. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97097/1/Euphytica-Polyploid-Grape-1213.pdf
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Marc: |
LEADER 02218naa a2200229 a 4500
001 1979552
005 2016-02-22
008 2014 bl uuuu u00u1 u #d
100 1 $aSINSKI, I.
245 $aImproving in vitro induction of autopolyploidy in grapevine seedless cultivars.$h[electronic resource]
260 $c2014
520 $aThe efficiency of in vitro polyploidization depends on several variables associated to the plant, the antimicrotubule agent and the interactions between them. In the present work, we have used response-surface methodology to determine the best operating conditions for plant recovery in polyploidization assays for shoot apices and somatic embryos of two seedless grape cultivars, employing colchicine and oryzalin. Explant type, tubulin-interfering compound and concentration were the critical factors determining plant recovery. Linear reduction in viable plant regeneration via organogenesis and somatic embryogenesis was obtained by increasing oryzalin concentrations and treatment time, whereas the effects of colchicine were better described by a quadratic design for both explants types. The conditions promoting higher rates of plant recovery were used in chromosome doubling experiments with oryzalin and colchicine for shoot apices and somatic embryos of ?Crimson seedless? and ?BRS Clara?. The established protocols allowed the recovery of non-chimerical autotetraploid plants at rates higher than 30 % for both cultivars. Stomata size parameters statistically correlate to the ploidy level of the regenerants and were effective for preliminary polyploidy screening. Autotetraploid lines of seedless grapes were incorporated into the Vitis germplasm bank for further agronomical evaluations. To our knowledge, this is the first report of in vitro oryzalin induced polyploidization of grapevine and of the use of mathematical modeling to optimize chromosome doubling in plants.
650 $aGenética
650 $aPoliploidia
650 $aUva
650 $aViticultura
700 1 $aBOSCO, D. D.
700 1 $aPIEROZZI, N. I.
700 1 $aMAIA, J. D. G.
700 1 $aRITSCHEL, P. S.
700 1 $aQUECINI, V.
773 $tEuphytica, Wageningen$gv. 196, n. 2, p. 299-311, 2014.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
21/12/1999 |
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Data da última atualização: |
17/04/2012 |
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Autoria: |
KATO, O. R.; ALBUQUERQUE, F. C. de; KATO, M. do S. A.; MASCARENHAS, R. E. B. |
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Afiliação: |
OSVALDO RYOHEI KATO, CPATU; FERNANDO CARNEIRO DE ALBUQUERQUE, CPATU; MARIA DO SOCORRO ANDRADE KATO, CPATU; Raimundo Evandro B. Nascarenhas, CPATU. |
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Título: |
Controle de ervas daninhas na cultura da pimenta-do-reino na região Transamazônia. |
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Ano de publicação: |
1981 |
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Fonte/Imprenta: |
Altamira: EMBRAPA-UEPAE Altamira, 1981. |
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Páginas: |
4 p. |
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Série: |
(EMBRAPA-UEPAE Altamira. Pesquisa em andamento, 2). |
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Idioma: |
Português |
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Conteúdo: |
A pimenta-do-reino (Piper nigrum L.) é uma das principais culturas que contribuem na economia do produtor rural do Estado do Pará. Desde 1933, quando foi introduzido o cultivo racional da pipericultura, até hoje, os custos de manutenção de um pimental têm aumentado em função da escassez de mão-de-obra, sendo a capina manual uma das operações que mais tem contribuído para este fato. |
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Palavras-Chave: |
Altamira; Brasil; Controle de erva daninha; Pará; Pimenta-do-reino; Transamazônica. |
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Thesagro: |
Controle Químico; Erva Daninha; Herbicida; Pimenta do Reino; Piper Nigrum. |
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Thesaurus NAL: |
Amazonia; weed control. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/57733/1/Altamira-PA2-1981.pdf
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Marc: |
LEADER 01299nam a2200325 a 4500
001 1379805
005 2012-04-17
008 1981 bl uuuu u0uu1 u #d
100 1 $aKATO, O. R.
245 $aControle de ervas daninhas na cultura da pimenta-do-reino na região Transamazônia.
260 $aAltamira: EMBRAPA-UEPAE Altamira$c1981
300 $a4 p.
490 $a(EMBRAPA-UEPAE Altamira. Pesquisa em andamento, 2).
520 $aA pimenta-do-reino (Piper nigrum L.) é uma das principais culturas que contribuem na economia do produtor rural do Estado do Pará. Desde 1933, quando foi introduzido o cultivo racional da pipericultura, até hoje, os custos de manutenção de um pimental têm aumentado em função da escassez de mão-de-obra, sendo a capina manual uma das operações que mais tem contribuído para este fato.
650 $aAmazonia
650 $aweed control
650 $aControle Químico
650 $aErva Daninha
650 $aHerbicida
650 $aPimenta do Reino
650 $aPiper Nigrum
653 $aAltamira
653 $aBrasil
653 $aControle de erva daninha
653 $aPará
653 $aPimenta-do-reino
653 $aTransamazônica
700 1 $aALBUQUERQUE, F. C. de
700 1 $aKATO, M. do S. A.
700 1 $aMASCARENHAS, R. E. B.
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Embrapa Amazônia Oriental (CPATU) |
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