|
|
Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
27/01/2014 |
Data da última atualização: |
09/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
COSTA, F. M.; SCHIAVO, J. A.; BRASIL, M. S.; LEITE, J.; XAVIER, G. R.; FERNANDES JUNIOR, P. I. |
Afiliação: |
Universidade Estadual do Mato Grosso do Sul; Universidade Estadual do Mato Grosso do Sul; Universidade Federal do Mato Grosso do Sul; Universidade Federal Rural do Rio de Janeiro; GUSTAVO RIBEIRO XAVIER, CNPAB; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
Título: |
Phenotypic and molecular finger printing of fast growing rhizobia of field-grown pigeonpea from the eastern edge of the Brazilian Pantanal. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 13, n. 1, p. 469-482 2014. |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeonpea plants grown at the eastern edge of the Brazilian Pantanal. The bacterial isolates were isolated from root nodules from field-growing pigeonpea grown in two rural settlements of the Aquidauana municipality. The bacterial isolates were characterized phenotypically by means of cultural characterization, intrinsic antibiotic resistance (IAR), salt and high incubation temperature tolerance, and amylolytic and cellulolytic activities. The molecular characterization of the bacterial isolates was carried out using amplified ribosomal DNA restriction analysis (ARDRA) and Box-polymerase chain reaction (PCR) techniques. In addition, the symbiotic performance of selected rhizobial isolates was evaluated in a greenhouse experiment using sterile substrate. The phenotypic characterization revealed that the bacterial strains obtained from pigeonpea root nodules presented characteristics that are uncommon among rhizobial isolates, indicating the presence of new species nodulating the pigeonpea plants in the Brazilian Pantanal. The molecular fingerprinting of these bacterial isolates also showed a highly diverse collection, with both techniques revealing less than 25% similarity among bacterial isolates. The evaluation of symbiotic performance also indicated the presence of microorganisms with high potential to increase the growth and nitrogen content at the shoots of pigeonpea plants. The results obtained in this study indicate the presence of a highly diversified rhizobial community nodulating the pigeonpea at the eastern edge of the Brazilian Pantanal. MenosThe aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeonpea plants grown at the eastern edge of the Brazilian Pantanal. The bacterial isolates were isolated from root nodules from field-growing pigeonpea grown in two rural settlements of the Aquidauana municipality. The bacterial isolates were characterized phenotypically by means of cultural characterization, intrinsic antibiotic resistance (IAR), salt and high incubation temperature tolerance, and amylolytic and cellulolytic activities. The molecular characterization of the bacterial isolates was carried out using amplified ribosomal DNA restriction analysis (ARDRA) and Box-polymerase chain reaction (PCR) techniques. In addition, the symbiotic performance of selected rhizobial isolates was evaluated in a greenhouse experiment using sterile substrate. The phenotypic characterization revealed that the bacterial strains obtained from pigeonpea root nodules presented characteristics that are uncommon among rhizobial isolates, indicating the presence of new species nodulating the pigeonpea plants in the Brazilian Pantanal. The molecular fingerprinting of these bacterial isolates also showed a highly diverse collection, with both techniques revealing less than 25% similarity among bacterial isolates. The evaluation of symbiotic performance also indicated the presence of microorganisms with high potential to increase the growth and nitrogen content at the shoots of pigeonpea p... Mostrar Tudo |
Palavras-Chave: |
Diversidade; Ecologia microbiana; Feijão guandu; Fixação biológica de nitrogênio; Nódulos radiculares; Rizóbio. |
Thesagro: |
Bactéria; Feijão; Fixação de Nitrogênio. |
Thesaurus Nal: |
Beans; Genetic disorders; Plant genetics. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/95854/1/Paulo-Ivan.pdf
|
Marc: |
LEADER 02669naa a2200325 a 4500 001 1977492 005 2023-01-09 008 2014 bl uuuu u00u1 u #d 100 1 $aCOSTA, F. M. 245 $aPhenotypic and molecular finger printing of fast growing rhizobia of field-grown pigeonpea from the eastern edge of the Brazilian Pantanal.$h[electronic resource] 260 $c2014 520 $aThe aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeonpea plants grown at the eastern edge of the Brazilian Pantanal. The bacterial isolates were isolated from root nodules from field-growing pigeonpea grown in two rural settlements of the Aquidauana municipality. The bacterial isolates were characterized phenotypically by means of cultural characterization, intrinsic antibiotic resistance (IAR), salt and high incubation temperature tolerance, and amylolytic and cellulolytic activities. The molecular characterization of the bacterial isolates was carried out using amplified ribosomal DNA restriction analysis (ARDRA) and Box-polymerase chain reaction (PCR) techniques. In addition, the symbiotic performance of selected rhizobial isolates was evaluated in a greenhouse experiment using sterile substrate. The phenotypic characterization revealed that the bacterial strains obtained from pigeonpea root nodules presented characteristics that are uncommon among rhizobial isolates, indicating the presence of new species nodulating the pigeonpea plants in the Brazilian Pantanal. The molecular fingerprinting of these bacterial isolates also showed a highly diverse collection, with both techniques revealing less than 25% similarity among bacterial isolates. The evaluation of symbiotic performance also indicated the presence of microorganisms with high potential to increase the growth and nitrogen content at the shoots of pigeonpea plants. The results obtained in this study indicate the presence of a highly diversified rhizobial community nodulating the pigeonpea at the eastern edge of the Brazilian Pantanal. 650 $aBeans 650 $aGenetic disorders 650 $aPlant genetics 650 $aBactéria 650 $aFeijão 650 $aFixação de Nitrogênio 653 $aDiversidade 653 $aEcologia microbiana 653 $aFeijão guandu 653 $aFixação biológica de nitrogênio 653 $aNódulos radiculares 653 $aRizóbio 700 1 $aSCHIAVO, J. A. 700 1 $aBRASIL, M. S. 700 1 $aLEITE, J. 700 1 $aXAVIER, G. R. 700 1 $aFERNANDES JUNIOR, P. I. 773 $tGenetics and Molecular Research$gv. 13, n. 1, p. 469-482 2014.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Semiárido (CPATSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Roraima. |
Data corrente: |
19/11/2014 |
Data da última atualização: |
01/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
ZILLI, J. E.; BARAÚNA, A. C.; SILVA, K. da; MEYER, S. E.; FARIAS, E. do N. C.; KAMINSKI, P.; COSTA, I. B. da; ARDLEY, J. K.; WILLEMS, A.; CAMACHO, N. N.; DOURADO, F. dos S.; O'HARA, G. |
Afiliação: |
JERRI EDSON ZILLI, CNPAB; NATALIA NEUTZLING CAMACHO, CNPAB. |
Título: |
Bradyrhizobium neotropicale sp. nov. isolated from effective nodules of Centrolobium paraense grown in soil of Amazonia, Brazil. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
International journal of Systematic and Evolutionary Microbiology, v. 64, pt 12, p. 3950-3957, 2014. |
ISSN: |
1466-5034 |
DOI: |
10.1099/ijs.0.065458-0 |
Idioma: |
Inglês Português |
Thesagro: |
Bactéria. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00868naa a2200277 a 4500 001 2002150 005 2023-09-01 008 2014 bl uuuu u00u1 u #d 022 $a1466-5034 024 7 $a10.1099/ijs.0.065458-0$2DOI 100 1 $aZILLI, J. E. 245 $aBradyrhizobium neotropicale sp. nov. isolated from effective nodules of Centrolobium paraense grown in soil of Amazonia, Brazil. 260 $c2014 650 $aBactéria 700 1 $aBARAÚNA, A. C. 700 1 $aSILVA, K. da 700 1 $aMEYER, S. E. 700 1 $aFARIAS, E. do N. C. 700 1 $aKAMINSKI, P. 700 1 $aCOSTA, I. B. da 700 1 $aARDLEY, J. K. 700 1 $aWILLEMS, A. 700 1 $aCAMACHO, N. N. 700 1 $aDOURADO, F. dos S. 700 1 $aO'HARA, G. 773 $tInternational journal of Systematic and Evolutionary Microbiology$gv. 64, pt 12, p. 3950-3957, 2014.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|