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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
17/12/2013 |
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Data da última atualização: |
17/12/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GAMA, R. N. C. S.; SANTOS, C. A. F.; DIAS, R. de C. S.; SOUZA, F. de F. |
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Afiliação: |
RENATA NATÁLIA C. S. GAMA, UEFS, Feira de Santana, BA; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; RITA DE CASSIA SOUZA DIAS, CPATSA; FLAVIO DE FRANCA SOUZA, CPATSA. |
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Título: |
Molecular characterization of watermelon cultivars using microsatellite markers. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Horticultura Brasileira, Brasília, DF, v. 31, n. 4, p. 522-527, out./dez. 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Allelic patterns and genetic similarity among 17 watermelon cultivars were established using microsatellite markers. For visualization of the genetic similarity, the dendrogram UPGMA (Unweighted Pair Group Method with Arithmetic Means) was generated by the similarity matrix of the Jacquard coefficient, based on 34 alleles of ten microsatellite loci. Total DNA was extracted by the CTAB 2x method and PCR (Polymerase Chain Reaction) products were analyzed in denaturing polyacrylamide 6% gels, stained with silver nitrate. The number of base pairs was estimated by the method of inverse mobility, based on known size product regression. Similarity ranged from 34 to 100%, reflecting high genetic variability. Analyzed loci were not enough to distinguish all 17 watermelon cultivars. The pairs ?Sugar Baby? and ?Omaru Yamato?, ?Charleston Gray? and ?Sunshade?, ?Crimson Sweet? and ?Nova Crimson? presented 100% of similarity. In dendrogram two groups were observed at 0.42 similarity cut point, with Citrullus colocynthis, positioned as an out group. One watermelon group was formed predominantly by cultivars derived from ?Crimson? and another group was formed by cultivars of different types such as ?Sugar Baby?, ?Charleston Gray? and ?Pérola?. Allele pattern and base pair (bp) estimates for the 34 alleles in the 10 microsatellite loci revealed in the present study are a first endeavor to use microsatellite markers in situations of cultivar protection for the watermelon agribusiness in Brazil. They can also be used in situations of commercial disputes regarding certification of the main watermelon cultivars used in the country. MenosAllelic patterns and genetic similarity among 17 watermelon cultivars were established using microsatellite markers. For visualization of the genetic similarity, the dendrogram UPGMA (Unweighted Pair Group Method with Arithmetic Means) was generated by the similarity matrix of the Jacquard coefficient, based on 34 alleles of ten microsatellite loci. Total DNA was extracted by the CTAB 2x method and PCR (Polymerase Chain Reaction) products were analyzed in denaturing polyacrylamide 6% gels, stained with silver nitrate. The number of base pairs was estimated by the method of inverse mobility, based on known size product regression. Similarity ranged from 34 to 100%, reflecting high genetic variability. Analyzed loci were not enough to distinguish all 17 watermelon cultivars. The pairs ?Sugar Baby? and ?Omaru Yamato?, ?Charleston Gray? and ?Sunshade?, ?Crimson Sweet? and ?Nova Crimson? presented 100% of similarity. In dendrogram two groups were observed at 0.42 similarity cut point, with Citrullus colocynthis, positioned as an out group. One watermelon group was formed predominantly by cultivars derived from ?Crimson? and another group was formed by cultivars of different types such as ?Sugar Baby?, ?Charleston Gray? and ?Pérola?. Allele pattern and base pair (bp) estimates for the 34 alleles in the 10 microsatellite loci revealed in the present study are a first endeavor to use microsatellite markers in situations of cultivar protection for the watermelon agribusiness in Brazi... Mostrar Tudo |
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Palavras-Chave: |
Divergência genética; Marcadores microssatélites; Melhoramento genético; Watermelon. |
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Thesagro: |
Citrullus Lanatus; Melancia. |
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Thesaurus Nal: |
Breeding. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94171/1/Carlos-3.pdf
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Marc: |
LEADER 02382naa a2200241 a 4500
001 1974131
005 2013-12-17
008 2013 bl uuuu u00u1 u #d
100 1 $aGAMA, R. N. C. S.
245 $aMolecular characterization of watermelon cultivars using microsatellite markers.$h[electronic resource]
260 $c2013
520 $aAllelic patterns and genetic similarity among 17 watermelon cultivars were established using microsatellite markers. For visualization of the genetic similarity, the dendrogram UPGMA (Unweighted Pair Group Method with Arithmetic Means) was generated by the similarity matrix of the Jacquard coefficient, based on 34 alleles of ten microsatellite loci. Total DNA was extracted by the CTAB 2x method and PCR (Polymerase Chain Reaction) products were analyzed in denaturing polyacrylamide 6% gels, stained with silver nitrate. The number of base pairs was estimated by the method of inverse mobility, based on known size product regression. Similarity ranged from 34 to 100%, reflecting high genetic variability. Analyzed loci were not enough to distinguish all 17 watermelon cultivars. The pairs ?Sugar Baby? and ?Omaru Yamato?, ?Charleston Gray? and ?Sunshade?, ?Crimson Sweet? and ?Nova Crimson? presented 100% of similarity. In dendrogram two groups were observed at 0.42 similarity cut point, with Citrullus colocynthis, positioned as an out group. One watermelon group was formed predominantly by cultivars derived from ?Crimson? and another group was formed by cultivars of different types such as ?Sugar Baby?, ?Charleston Gray? and ?Pérola?. Allele pattern and base pair (bp) estimates for the 34 alleles in the 10 microsatellite loci revealed in the present study are a first endeavor to use microsatellite markers in situations of cultivar protection for the watermelon agribusiness in Brazil. They can also be used in situations of commercial disputes regarding certification of the main watermelon cultivars used in the country.
650 $aBreeding
650 $aCitrullus Lanatus
650 $aMelancia
653 $aDivergência genética
653 $aMarcadores microssatélites
653 $aMelhoramento genético
653 $aWatermelon
700 1 $aSANTOS, C. A. F.
700 1 $aDIAS, R. de C. S.
700 1 $aSOUZA, F. de F.
773 $tHorticultura Brasileira, Brasília, DF$gv. 31, n. 4, p. 522-527, out./dez. 2013.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 528 | |
| 161. |  | COSTAMILAN, L. M.; CLEBSCH, C. C.; SEIXAS, C. D. S.; SOARES, R. M.; GODOY, C. V. Caracterização da diversidade da população patogênica de Phytophthora sojae do Brasil. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 33., 2013, Londrina. Resumos expandidos... Brasília, DF: Embrapa, 2013. p. 77-79.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 163. | | DALBOSCO, J.; PAVAN, W.; DELPONTE, E.; GODOY, C. V.; CERVI, C. R. Aplicações ricas no controle de doenças da soja. In: CONGRESSO BRASILEIRO DE AGROINFORMÁTICA, 8., 2011, Bento Gonçalves. Anais... Florianópolis: UFSC; Pelotas: UFPel, 2011. 5 p. Seção Poster II, pdf 89833_1. CD-ROM. SBIAGRO.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 165. | | GODOY, C. V.; FERNANDES, C. D.; FLAUSINO, A. M.; PEREIRA, T. B. C. Avaliação da reação de acessos de espécies de estilosantes a Phakopsora pachyrhizi. In: CONGRESSO BRASILEIRO DE SOJA, 8., 2018, Goiânia. Inovação, tecnologias digitais e sustentabilidade da soja: anais. Brasília, DF: Embrapa, 2018. p. 565-567.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 166. | | COSTAMILAN, L. M.; GODOY, C. V.; YORINORI, J. T.; GUGEL, F. L. Avaliação da reação de genótipos de soja à ferrugem. In: BERTAGNOLLI, P. F.; COSTAMILAN, L. M. (Org.). Soja: resultados de pesquisa 2003/2004. Passo Fundo: Embrapa Trigo, 2004. p. 88-91. (Embrapa Trigo. Documentos, 50). Trabalhos apresentados na XXXII Reunião de Pesquisa de Soja da Região Sul, Passo Fundo, jul. 2004.| Biblioteca(s): Embrapa Soja. |
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| 167. | | COSTAMILAN, L. M.; GODOY, C. V.; YORINORI, J. T.; GUGEL, F. L. Avaliação da reação de genótipos de soja à ferrugem. Fitopatologia Brasileira, Brasília, v. 29, p. S137, ago. 2004. Suplemento. Resumo apresentado no XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, agosto, 2004.| Biblioteca(s): Embrapa Soja. |
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| 168. | | ZAMBENEDETTI, E. B.; ALVES, E.; POZZA, E. A.; ARAÚJO, D. V. de; GODOY, C. V. Avaliação de parâmetros monocíclicos e da densidade da ferrugem asiática (Phakopsora pachyrhizi) em diferentes genótipos de soja e posições de copa. Summa Phytopathologica, Botucatu, v. 33, n. 2, p. 178-181, abr./jun. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - C |
| Biblioteca(s): Embrapa Soja. |
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| 169. | | SOARES, R. M.; ALMEIDA FILHO, K. M.; MEYER, M. C.; TERAMOTO, A.; GODOY, C. V. Comparação da virulência de isolados de corynespora cassiicola obtidos em soja. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: resumos. Brasília, DF: Embrapa, 2012. p. 118, res. 194.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 170. | | SOARES, R. M.; ALMEIDA FILHO, K. M.; MEYER, M. C.; TERAMOTO, A.; GODOY, C. V. Comparação da virulência de isolados de corynespora cassiicola obtidos em soja. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. 4 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 174. | | DEL PONTE, E.; GODOY, C.; PREEZ, E. du; RIJ, N. van; CALDWELL, P.; YANG, X. Predicting regional Asian soybean rust outbreaks in Brazil and South Africa. Phytopathology, St. Paul, v. 95, n. 6, p. S23, June 2005. Supplement. Edição dos Resumos da APS Annual Meeting, Austin, TX, July/Aug. 2005. Nome correto do segundo autor GODOY, C. V.| Biblioteca(s): Embrapa Soja. |
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| 176. | | GODOY, C.; SOARES, R.; SEIXAS, C.; PONTE, E. del; SPOLTI, P.; SANTOS, T. dos. Precoce e agressiva. Cultivar: grandes culturas, Pelotas, v. 12, n. 129, p. 26-28, fev. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Soja. |
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| 180. | | HIKISHIMA, M.; CANTERI, M. G.; GODOY, C. V.; KOGA, L. J.; SILVA, A. J. da. Quantificação de danos e relações entre severidade, medidas de refletância e produtividade no patossistema ferrugem asiática da soja. Tropical Plant Pathology, Brasília, DF, v. 35, n. 2, p. 96-103, mar./apr. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 528 | |
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