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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
21/06/2013 |
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Data da última atualização: |
12/04/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
DUBIELA, C. R.; FAJARDO, T. V. M.; SOUTO, E. R.; NICKEL, O.; EIRAS, M.; REVERS, L. F. |
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Afiliação: |
CARLA R. DUBIELA, UEM; THOR VINICIUS MARTINS FAJARDO, CNPUV; ELIEZER R. SOUTO, UEM; OSMAR NICKEL, CNPUV; MARCELO EIRAS, INSTITUTO BIOLÓGICO; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Simultaneous detection of Brazilian isolates of grapevine viruses by TaqMan real-time RT-PCR. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Tropical Plant Pathology, Viçosa, v. 38, n. 2, p. 158-165, mar./apr. 2013 |
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Idioma: |
Português |
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Notas: |
Comunicação científica. |
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Conteúdo: |
The aim of this work was to evaluate the efficiency of real-time RT-PCR for detection of different isolates of ten important virus species that infect grapevines in Brazil: Grapevine leafroll-associated virus (GLRaV-1, -2, -3 and -5), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine fleck virus (GFkV) and Grapevine fanleaf virus (GFLV). The reactions consisted of individual (simplex) and simultaneous (duplex) virus detections. Thirty six grapevine accessions, regenerated after thermotherapy and tissue culture treatments, have been analysed. All the above-mentioned viruses were sensitively detected in simplex reactions in samples infected with different virus isolates. Specifically to GLRaV-1 it was necessary to mix reagents refered by different sources to achieve the amplification. GVA, GRSPaV, GLRaV-2 and GLRaV-3 combined with GVB, GFLV, GFkV, GVD and GLRaV-5 were accurately detected in duplex trials. It was shown, that real-time RT-PCR (TaqMan) is able to efficiently detect different local virus species and isolates. |
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Thesagro: |
Diagnostico; Doença de planta; Uva; Vírus; Viticultura. |
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Thesaurus Nal: |
Betaflexiviridae; Closteroviridae. |
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Categoria do assunto: |
-- |
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URL: |
https://www.scielo.br/pdf/tpp/v38n2/a11v38n2.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/84658/1/DUBIELA-TPP-v38n2p158-2013.pdf
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Marc: |
LEADER 01917nam a2200265 a 4500
001 1960405
005 2019-04-12
008 2013 bl uuuu u00u1 u #d
100 1 $aDUBIELA, C. R.
245 $aSimultaneous detection of Brazilian isolates of grapevine viruses by TaqMan real-time RT-PCR.$h[electronic resource]
260 $aTropical Plant Pathology, Viçosa, v. 38, n. 2, p. 158-165, mar./apr. 2013$c2013
500 $aComunicação científica.
520 $aThe aim of this work was to evaluate the efficiency of real-time RT-PCR for detection of different isolates of ten important virus species that infect grapevines in Brazil: Grapevine leafroll-associated virus (GLRaV-1, -2, -3 and -5), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine fleck virus (GFkV) and Grapevine fanleaf virus (GFLV). The reactions consisted of individual (simplex) and simultaneous (duplex) virus detections. Thirty six grapevine accessions, regenerated after thermotherapy and tissue culture treatments, have been analysed. All the above-mentioned viruses were sensitively detected in simplex reactions in samples infected with different virus isolates. Specifically to GLRaV-1 it was necessary to mix reagents refered by different sources to achieve the amplification. GVA, GRSPaV, GLRaV-2 and GLRaV-3 combined with GVB, GFLV, GFkV, GVD and GLRaV-5 were accurately detected in duplex trials. It was shown, that real-time RT-PCR (TaqMan) is able to efficiently detect different local virus species and isolates.
650 $aBetaflexiviridae
650 $aClosteroviridae
650 $aDiagnostico
650 $aDoença de planta
650 $aUva
650 $aVírus
650 $aViticultura
700 1 $aFAJARDO, T. V. M.
700 1 $aSOUTO, E. R.
700 1 $aNICKEL, O.
700 1 $aEIRAS, M.
700 1 $aREVERS, L. F.
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Embrapa Uva e Vinho (CNPUV) |
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| 1. |  | DUBIELA, C. R.; FAJARDO, T. V. M.; SOUTO, E. R.; NICKEL, O.; EIRAS, M.; REVERS, L. F. Simultaneous detection of Brazilian isolates of grapevine viruses by TaqMan real-time RT-PCR. Tropical Plant Pathology, Viçosa, v. 38, n. 2, p. 158-165, mar./apr. 2013 Comunicação científica.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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