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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
18/06/2012 |
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Data da última atualização: |
24/07/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRIGUES, F. A.; MARCOLINO-GOMES, J.; CARVALHO, J. de F. C.; NASCIMENTO, L. C. do; NEUMAIER, N.; FARIAS, J. R. B.; CARAZZOLLE, M. F.; MARCELINO, F. C.; NEPOMUCENO, A. L. |
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Afiliação: |
FABIANA APARECIDA RODRIGUES, CNPSo; JULIANA MARCOLINO-GOMES, CNPSo; JOSIRLEI DE FÁTIMA CORRÊA CARVALHO, CARVALHO , J. de F. C.; LEANDRO COSTA DO NASCIMENTO, UNICAMP; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARCELO FALSARELLA CARAZZOLLE, UNICAMP; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI. |
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Título: |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 304-314, May 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress. |
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Thesagro: |
Deficiência hídrica; Estiagem; Gene; Genoma; Relação água-planta; Seca; Soja. |
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Thesaurus Nal: |
Drought; Gene expression; Soybeans. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/61091/1/gmb.subtractive.v35n1s.304-314.2012.pdf
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Marc: |
LEADER 02370naa a2200337 a 4500
001 1926619
005 2012-07-24
008 2012 bl uuuu u00u1 u #d
100 1 $aRODRIGUES, F. A.
245 $aSubtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.
260 $c2012
520 $aSoybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.
650 $aDrought
650 $aGene expression
650 $aSoybeans
650 $aDeficiência hídrica
650 $aEstiagem
650 $aGene
650 $aGenoma
650 $aRelação água-planta
650 $aSeca
650 $aSoja
700 1 $aMARCOLINO-GOMES, J.
700 1 $aCARVALHO, J. de F. C.
700 1 $aNASCIMENTO, L. C. do
700 1 $aNEUMAIER, N.
700 1 $aFARIAS, J. R. B.
700 1 $aCARAZZOLLE, M. F.
700 1 $aMARCELINO, F. C.
700 1 $aNEPOMUCENO, A. L.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 35, n. 1, suppl., p. 304-314, May 2012.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/11/2015 |
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Data da última atualização: |
15/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FAVERO, A. P.; SANTOS, R. F. dos; SIMPSON, C. E.; VALLS, J. F. M.; VELLO, N. A. |
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Afiliação: |
ALESSANDRA PEREIRA FAVERO, CPPSE; RODRIGO FURTADO DOS SANTOS, CNPAE; CHARLES E. SIMPSON, TEXAS AGRILIFE RESEARCH -TEXAS A&M UNIVERSITY/STEPHENVILLE; JOSE FRANCISCO MONTENEGRO VALLS, CENARGEN; NATAL ANTONIO VELLO, ESALQ/PIRACICABA. |
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Título: |
Successful crosses between fungal-resistant wild species of Arachis (section Arachis) and Arachis hypogaea. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, v. 38, n. 3, p. 353-365, sep. 2015. |
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DOI: |
10.1590/S1415-475738320140376 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
Peanut (Arachis hypogaea) is the fifth most produced oil crop worldwide. Besides lack of water, fungal diseases are the most limiting factors for the crop. Several species of Arachis are resistant to certain pests and diseases. This study aimed to successfully cross the A-genome with B-K-A genome wild species previously selected for fungal disease resistance, but that are still untested. We also aimed to polyplodize the amphihaploid chromosomes; cross the synthetic amphidiploids and A. hypogaea to introgress disease resistance genes into the cultivated peanut; and analyze pollen viability and morphological descriptors for all progenies and their parents. We selected 12 A-genome accessions as male parents and three B-genome species, one K-genome species, and one A-genome species as female parents. Of the 26 distinct cross combinations, 13 different interspecific AB-genome and three AA-genome hybrids were obtained. These sterile hybrids were polyploidized and five combinations produced tetraploid flowers. Next, 16 combinations were crossed between A. hypogaea and the synthetic amphidiploids, resulting in 11 different hybrid combinations. Our results confirm that it is possible to introgress resistance genes from wild species into the peanut using artificial hybridization, and that more species than previously reported can be used, thus enhancing the genetic variability in peanut genetic improvement programs. |
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Palavras-Chave: |
Interspecific hybrids; Peanut; Pollen viability; Polyploidization. |
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Thesaurus NAL: |
colchicine. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/133604/1/alessamdrafavero.pdf
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Marc: |
LEADER 02181naa a2200241 a 4500
001 2029193
005 2023-03-15
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1590/S1415-475738320140376$2DOI
100 1 $aFAVERO, A. P.
245 $aSuccessful crosses between fungal-resistant wild species of Arachis (section Arachis) and Arachis hypogaea.$h[electronic resource]
260 $c2015
520 $aPeanut (Arachis hypogaea) is the fifth most produced oil crop worldwide. Besides lack of water, fungal diseases are the most limiting factors for the crop. Several species of Arachis are resistant to certain pests and diseases. This study aimed to successfully cross the A-genome with B-K-A genome wild species previously selected for fungal disease resistance, but that are still untested. We also aimed to polyplodize the amphihaploid chromosomes; cross the synthetic amphidiploids and A. hypogaea to introgress disease resistance genes into the cultivated peanut; and analyze pollen viability and morphological descriptors for all progenies and their parents. We selected 12 A-genome accessions as male parents and three B-genome species, one K-genome species, and one A-genome species as female parents. Of the 26 distinct cross combinations, 13 different interspecific AB-genome and three AA-genome hybrids were obtained. These sterile hybrids were polyploidized and five combinations produced tetraploid flowers. Next, 16 combinations were crossed between A. hypogaea and the synthetic amphidiploids, resulting in 11 different hybrid combinations. Our results confirm that it is possible to introgress resistance genes from wild species into the peanut using artificial hybridization, and that more species than previously reported can be used, thus enhancing the genetic variability in peanut genetic improvement programs.
650 $acolchicine
653 $aInterspecific hybrids
653 $aPeanut
653 $aPollen viability
653 $aPolyploidization
700 1 $aSANTOS, R. F. dos
700 1 $aSIMPSON, C. E.
700 1 $aVALLS, J. F. M.
700 1 $aVELLO, N. A.
773 $tGenetics and Molecular Biology$gv. 38, n. 3, p. 353-365, sep. 2015.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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