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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
22/03/2012 |
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Data da última atualização: |
22/03/2012 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, M. S.; GHINI, R.; FERNANDES, B. V. |
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Afiliação: |
M. S. SANTOS, FCA-UNESP; RAQUEL GHINI, CNPMA; B. V. FERNANDES, V&M Florestal Ltda. |
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Título: |
Impacto do aumento da concentração de dióxido de carbono sobre a murcha de Ceratocystis em dois híbridos de Eucalyptus. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, DF, v. 36, p. 0780, ago. 2011. Suplemento. Edição dos resumos do XLIV Congresso Brasileiro de Fitopatologia, Bento Gonçalves, ago. 2010. Resumo 1152. |
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Idioma: |
Português |
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Palavras-Chave: |
Mudanças climáticas e doenças de plantas; Murcha de Ceratocystis. |
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Thesagro: |
Ceratocystis Fimbriata; Controle biologico; Dióxido de Carbono; Eucalipto. |
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Thesaurus Nal: |
Eucalyptus. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/56219/1/2011RA115.pdf
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Marc: |
LEADER 00848nam a2200205 a 4500
001 1919925
005 2012-03-22
008 2011 bl uuuu u00u1 u #d
100 1 $aSANTOS, M. S.
245 $aImpacto do aumento da concentração de dióxido de carbono sobre a murcha de Ceratocystis em dois híbridos de Eucalyptus.$h[electronic resource]
260 $aTropical Plant Pathology, Brasília, DF, v. 36, p. 0780, ago. 2011. Suplemento. Edição dos resumos do XLIV Congresso Brasileiro de Fitopatologia, Bento Gonçalves, ago. 2010. Resumo 1152.$c1152
650 $aEucalyptus
650 $aCeratocystis Fimbriata
650 $aControle biologico
650 $aDióxido de Carbono
650 $aEucalipto
653 $aMudanças climáticas e doenças de plantas
653 $aMurcha de Ceratocystis
700 1 $aGHINI, R.
700 1 $aFERNANDES, B. V.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
LOPES, F. G.; COSTA, E. P. DA; QUEIROZ-CASTRO, V. L. D.; PEREIRA, E. C. M.; GUIMARÃES, J. D.; ALVES, S. V. P.; FERNANDES, C. A. C.; CAMARGO, L. S. de A.; BENJAMIM, L. DOS A. |
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Afiliação: |
Flavio Guisseli Lopes; Eduardo Paulino da Costa; Vanessa Lopes Dias Queiroz-Castro; Emilio Cesar Martins Pereira; José Domingos Guimarães; Saullo Vinicius Pereira Alves; Carlos Antonio Carvalho Fernandes; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Laercio dos Anjos Benjamim. |
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Título: |
Use of two new formulations as bovine embryo manipulation solution. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Animal Reproduction, v. 16 n. 2, p. 348-355, 2019. |
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DOI: |
10.21451/1984-3143-ar2018-0044 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stages of early blastocyst, and compact morula grades I and II. These embryos were initially randomly distributed and maintained for four hours in three solutions: Modified phosphate buffered saline (PBS; Control); EMS (treatment 1), and EMS supplemented (treatment 2). Subsequently, they were cultured in TCM 199 medium and evaluated in terms of total number of cells, morphometric characteristics, ultra structural aspects, detection of cell apoptosis, and quantification of Hsp70.3 gene expression. In the second phase, these same solutions were tested in the transfer of quality I and II bovine embryos (excellent and good). These embryos were transferred fresh to 58 recipients. The results showed that the total number of cells in embryos expanded blastocyst (ExB), the number of apoptotic cells, the cell, nuclear, nucleolar diameter and the nucleus/nucleolus ratio was similar among the treatments. The pregnancy rate shown on second phase was also similar. However, the EMS supplemented expressed more Hsp70.3 than EMS. The expression of Hsp70.3 was also greater for embryos in EMS than that of EMS supplemented. The McII embryos, EMS and EMS supplemented samples also expressed more Hsp70.3 compared to control embryos. In conclusion, the tested solutions can be used in routine embryo transfer techniques, replacing modified PBS solution as an effective media in maintaining embryo viability. MenosABSTRACT This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stages of early blastocyst, and compact morula grades I and II. These embryos were initially randomly distributed and maintained for four hours in three solutions: Modified phosphate buffered saline (PBS; Control); EMS (treatment 1), and EMS supplemented (treatment 2). Subsequently, they were cultured in TCM 199 medium and evaluated in terms of total number of cells, morphometric characteristics, ultra structural aspects, detection of cell apoptosis, and quantification of Hsp70.3 gene expression. In the second phase, these same solutions were tested in the transfer of quality I and II bovine embryos (excellent and good). These embryos were transferred fresh to 58 recipients. The results showed that the total number of cells in embryos expanded blastocyst (ExB), the number of apoptotic cells, the cell, nuclear, nucleolar diameter and the nucleus/nucleolus ratio was similar among the treatments. The pregnancy rate shown on second phase was also similar. However, the EMS supplemented expressed more Hsp70.3 than EMS. The expression of Hsp70.3 was also greater for embryos in EMS than that of EMS supplemented. The McII embryos, EMS ... Mostrar Tudo |
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Palavras-Chave: |
Cell apoptosis; Hsp 70 ponto 3. |
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Thesaurus NAL: |
Mice. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208745/1/Use-of-two-new-formulations.pdf
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Marc: |
LEADER 02538naa a2200265 a 4500
001 2118720
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.21451/1984-3143-ar2018-0044$2DOI
100 1 $aLOPES, F. G.
245 $aUse of two new formulations as bovine embryo manipulation solution.$h[electronic resource]
260 $c2019
520 $aABSTRACT This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stages of early blastocyst, and compact morula grades I and II. These embryos were initially randomly distributed and maintained for four hours in three solutions: Modified phosphate buffered saline (PBS; Control); EMS (treatment 1), and EMS supplemented (treatment 2). Subsequently, they were cultured in TCM 199 medium and evaluated in terms of total number of cells, morphometric characteristics, ultra structural aspects, detection of cell apoptosis, and quantification of Hsp70.3 gene expression. In the second phase, these same solutions were tested in the transfer of quality I and II bovine embryos (excellent and good). These embryos were transferred fresh to 58 recipients. The results showed that the total number of cells in embryos expanded blastocyst (ExB), the number of apoptotic cells, the cell, nuclear, nucleolar diameter and the nucleus/nucleolus ratio was similar among the treatments. The pregnancy rate shown on second phase was also similar. However, the EMS supplemented expressed more Hsp70.3 than EMS. The expression of Hsp70.3 was also greater for embryos in EMS than that of EMS supplemented. The McII embryos, EMS and EMS supplemented samples also expressed more Hsp70.3 compared to control embryos. In conclusion, the tested solutions can be used in routine embryo transfer techniques, replacing modified PBS solution as an effective media in maintaining embryo viability.
650 $aMice
653 $aCell apoptosis
653 $aHsp 70 ponto 3
700 1 $aCOSTA, E. P. DA
700 1 $aQUEIROZ-CASTRO, V. L. D.
700 1 $aPEREIRA, E. C. M.
700 1 $aGUIMARÃES, J. D.
700 1 $aALVES, S. V. P.
700 1 $aFERNANDES, C. A. C.
700 1 $aCAMARGO, L. S. de A.
700 1 $aBENJAMIM, L. DOS A.
773 $tAnimal Reproduction$gv. 16 n. 2, p. 348-355, 2019.
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