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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
27/02/2012 |
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Data da última atualização: |
26/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARCELLOS, F. G.; HUNGRIA, M.; PIZZIRANI-KLEINER, A. A. |
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Afiliação: |
FERNANDO GOMES BARCELLOS, Universidade Paranaense - Umuarama, PR.; MARIANGELA HUNGRIA DA CUNHA, CNPSO; ALINE APARECIDA PIZZIRANI-KLEINER, USP - ESALQ. |
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Título: |
Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 42, n. 4, p. 1625-1637, Oct./Dec. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
With the aim of a better characterization of the somatic recombination process in Trichoderma pseudokoningii, a progeny from crossings between T. pseudokoningii strains contrasting for auxotroph markers was characterized by RAPD markers and PFGE (electrophoretic karyotype). Cytological studies of the conidia, conidiogenesis and heterokaryotic colonies were also performed. The genotypes of the majority of the recombinant strains analyzed were similar to only one of the parental strains and the low frequency of polymorphic RAPD bands suggested that the nuclear fusions may not occur into the heterokaryon. In some heterokaryotic regions the existence of intensely staining hyphae might be related to cell death. We proposed that a mechanism of somatic recombination other than parasexuality might occur, being related to limited vegetative compatibility after postfusion events, as described for other Trichoderma species. |
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Palavras-Chave: |
Somatic recombination. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/54698/1/limited.pdf
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Marc: |
LEADER 01449naa a2200157 a 4500
001 1916731
005 2017-07-26
008 2011 bl uuuu u00u1 u #d
100 1 $aBARCELLOS, F. G.
245 $aLimited vegetative compatibility as a cause of somatic recombination in$bTrichoderma pseudokoningii.
260 $c2011
520 $aWith the aim of a better characterization of the somatic recombination process in Trichoderma pseudokoningii, a progeny from crossings between T. pseudokoningii strains contrasting for auxotroph markers was characterized by RAPD markers and PFGE (electrophoretic karyotype). Cytological studies of the conidia, conidiogenesis and heterokaryotic colonies were also performed. The genotypes of the majority of the recombinant strains analyzed were similar to only one of the parental strains and the low frequency of polymorphic RAPD bands suggested that the nuclear fusions may not occur into the heterokaryon. In some heterokaryotic regions the existence of intensely staining hyphae might be related to cell death. We proposed that a mechanism of somatic recombination other than parasexuality might occur, being related to limited vegetative compatibility after postfusion events, as described for other Trichoderma species.
653 $aSomatic recombination
700 1 $aHUNGRIA, M.
700 1 $aPIZZIRANI-KLEINER, A. A.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 42, n. 4, p. 1625-1637, Oct./Dec. 2011.
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