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Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
29/12/2022 |
Data da última atualização: |
15/05/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
KATAYAMA, E.; RODRIGUES, N. A.; BILATTO, S.; CASCIATORI, F. P.; FARINAS, C. S. |
Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
Título: |
Nanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Biomass Conversion and Biorefnery, v. 14, n. 8, 2022. |
Páginas: |
9121-9136 |
DOI: |
https://doi.org/10.1007/s13399-022-02977-1 |
Idioma: |
Inglês |
Conteúdo: |
The isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept. MenosThe isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stabili... Mostrar Tudo |
Palavras-Chave: |
Endoglucanase; Packed-bed bioreactor; Solid-state cultivation. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02537naa a2200229 a 4500 001 2150493 005 2024-05-15 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s13399-022-02977-1$2DOI 100 1 $aKATAYAMA, E. 245 $aNanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor.$h[electronic resource] 260 $c2022 300 $a9121-9136 520 $aThe isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept. 653 $aEndoglucanase 653 $aPacked-bed bioreactor 653 $aSolid-state cultivation 700 1 $aRODRIGUES, N. A. 700 1 $aBILATTO, S. 700 1 $aCASCIATORI, F. P. 700 1 $aFARINAS, C. S. 773 $tBiomass Conversion and Biorefnery$gv. 14, n. 8, 2022.
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1. | | SANTOS, A. D. dos; SOUZA, M. I. F.; LEITE, M. A. de A.; MOURA, M. F.; CRUZ, S. A. B. da; SOUZA, K. X. S. de; MACÁRIO, C. G. do N.; MASSRUHÁ, S. M. F. S. Agência de informação Embrapa: acesso à informação tecnológica. In: CONGRESSO BRASILEIRO DE AGROINFORMÁTICA, 5.; SIMPÓSIO BRASILEIRO DE TECNOLOGIA DA INFORMAÇÃO NO AGRONEGÓCIO COOPERATIVO, 2., 2005, Londrina. Agronegócio, tecnologia e inovação: anais. Londrina: SBI-Agro, 2005. 3 p. Na publicação: Maria Angélica Andrade Leite.Biblioteca(s): Embrapa Agricultura Digital. |
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2. | | GABARDO, S.; RITSCHEL, P. S.; GARRIDO, L. da R.; SONEGO, O. R. Análise do polimorfismo da região ITS do rDNA de Cylindrocarpon spp., agente causal do pé-preto da videira utilizando endonucleases. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 5., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 1., 2007, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2007. p. 25. (Embrapa Uva e Vinho. Documentos, 63). Resumo.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Uva e Vinho. |
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4. | | DANTAS, J. L. L. Propagação rápida da bananeira. Cruz das Almas: Embrapa Mandioca e Fruticultura, 1986. 9 p. III Curso Intensivo Nacional de Fruticultura realizado em Cruz das Almas, BA. 1986.Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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7. | | MENEZES SOBRINHO, J. A. de; LOPES, C. A.; REIFSCHNEIDER, F. J. B.; CHARCHAR, J. M.; CRISOSTOMO, L. A.; CARRIJO, O. A.; BARBOSA, S. A cultura do alho. Brasília, DF: EMBRAPA SPI, 1993. 50 p. (EMBRAPA-SPI. Coleção Plantar, 1).Biblioteca(s): Embrapa Unidades Centrais. |
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1. | | 0900875, RELATORIO TECNICO ANUAL. EMBRAPA. CNPSA, EMBRAPA-CNPSA, Concordia-SC Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Acre; Embrapa Amapá; Embrapa Amazônia Oriental; Embrapa Pantanal. | |
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