Data/hora: 19/06/2024 11:54:20

Biblioteca(s): | Embrapa Instrumentação.
Data corrente: | 29/12/2022
Data da última atualização: | 15/05/2024
Autoria: | KATAYAMA, E.; RODRIGUES, N. A.; BILATTO, S.; CASCIATORI, F. P.; FARINAS, C. S.
Afiliação: | CRISTIANE SANCHEZ FARINAS, CNPDIA.
Título: | Nanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor.
Ano de publicação: | 2022
Fonte/Imprenta: | Biomass Conversion and Biorefnery, v. 14, n. 8, 2022.
Páginas: | 9121-9136
DOI: | https://doi.org/10.1007/s13399-022-02977-1
Idioma: | Inglês
Conteúdo: | The isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept.
Palavras-Chave: | Endoglucanase; Packed-bed bioreactor; Solid-state cultivation.
Categoria do assunto: | --
Registro original: | Embrapa Instrumentação (CNPDIA)
Exemplares: | CNPDIA - (18178-1) - UPC - AP - DD - PROCI.22/196 -  - 2022/204 - DIS