Data/hora: 08/06/2024 08:42:00

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	26/02/1996	
Data da última atualização: 	23/06/2023	
Autoria: 	ELLIS, J. A.; HAWK, D. A.; MILLS, K. W.; PRATT, D. L.	
Título: 	Antigen specificity and activity of ovine antibodies induced by immunization with Corybebacterium Pseudotuberculosis culture filtrate.	
Ano de publicação: 	1991	
Fonte/Imprenta: 	Veterinary Immunology and Immunopathology, v. 28, n. 3/4, p. 303-316, 1991.	
DOI: 	https://doi.org/10.1016/0165-2427(91)90122-S	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Sheep were immunized three times with a vaccine composed of filtrate from a 36 h culture of Corynebacterium pseudotuberculosis and a block polymer adjuvant. Immunization resulted in the development of exotoxin-neutralizing antibody. This corresponded to the recognition of a 31.6 kDa protein on sequential immunoblots of ammonium sulfate-precipitated filtrate. In addition, sera from vaccinated sheep recognized at least eight bacterial cellular antigens on immunoblots of ether-extracted C. pseudotuberculosis, including bands of 12, 25.1, 31.6, 36.3, 39.8, 63.1, 70, 75 or 79.4 kDa. Sera from these sheep altered the colony growth characteristics of C. pseudotuberculosis in vitro. These results indicate that immunization with soluble C. pseudotuberculosis antigen preparations that have been used in toxoid vaccines induces antibody responses to numerous cellular antigens in addition to exotoxin and suggest that serologically mediated antibacterial effects could be an important component in the protection from disease that has been reported following immunization with C. pseudotuberculosis toxoids.	
Thesagro: 	Corynebacterium Pseudotuberculosis; Doença; Linfadenite Caseosa; Ovino.	
Thesaurus Nal: 	Bacterial antibodies; Bacterial vaccines; Biosynthesis; Disease control; Disease prevention; Enzyme-linked immunosorbent assay; Immunoblotting; Immunology; Molecular weight; Polyacrylamide gel electrophoresis; Sheep diseases.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (9478-1) - ADD - AP - DD -  -  -  - DIS