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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Café; Embrapa Unidades Centrais. |
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Data corrente: |
20/04/2010 |
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Data da última atualização: |
10/03/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MISSIO, R. F.; CAIXETA, E. T.; ZAMBOLIM, E. M.; ZAMBOLIM, L.; SAKIYAMA, N. S. |
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Afiliação: |
ROBSON FERNANDO MISSIO, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA, SAPC; EUNIZE MACIEL ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; NEY SUSSUMU SAKIYAMA, UNIVERSIDADE FEDERAL DE VIÇOSA. |
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Título: |
Development and validation of SSR markers for Coffea arabica L. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In.: CROP BREEDING AND APPLIED BIOTECHNOLOGY. v.9, p.361-371, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1% of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSR markers were validated for further genetic studies of C. arabica. |
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Palavras-Chave: |
Biblioteca genômica enriquecida; Coffea arabica L; Coffee; Enriched genomic library; Marcador microssatélite; Molecular marker; SSR marker. |
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Thesagro: |
Café; Marcador Molecular. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29283/1/Development-and-validation.pdf
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Marc: |
LEADER 01553naa a2200277 a 4500
001 1880403
005 2011-03-10
008 2009 bl uuuu u00u1 u #d
100 1 $aMISSIO, R. F.
245 $aDevelopment and validation of SSR markers for Coffea arabica L.
260 $c2009
520 $aWith the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1% of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSR markers were validated for further genetic studies of C. arabica.
650 $aCafé
650 $aMarcador Molecular
653 $aBiblioteca genômica enriquecida
653 $aCoffea arabica L
653 $aCoffee
653 $aEnriched genomic library
653 $aMarcador microssatélite
653 $aMolecular marker
653 $aSSR marker
700 1 $aCAIXETA, E. T.
700 1 $aZAMBOLIM, E. M.
700 1 $aZAMBOLIM, L.
700 1 $aSAKIYAMA, N. S.
773 $tIn.: CROP BREEDING AND APPLIED BIOTECHNOLOGY.$gv.9, p.361-371, 2009.
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Registro original: |
Embrapa Café (CNPCa) |
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