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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
14/02/2011 |
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Data da última atualização: |
13/06/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
RIBEIRO, R. A.; HUNGRIA, M. |
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Afiliação: |
RENAN AUGUSTO RIBEIRO, UEL; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
MLSA (Multilocus Sequence Analysis) of brazilian N2-fixing symbionts of common bean (Phaseolus vulgaris L.). |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON CULTURE COLLECTIONS, 12., 2010, Florianópolis. Biological Resource Centrers: gateway to biodiversity and services for innovations in biotechnology: proceedings. [S.l]: WFCC, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Rhizobia are bacteria that live in symbiosis with legumes. In Brazil, the species Rhizobium tropici represents the majority of the population isolated from common bean (Phaseolus vulgaris L.) nodules of field grown plants. Since the description of the R. tropici species in 1991, several strains have shown variability in genetic and physiological properties, splitting the strains into two subgroups, R. tropici type A and type B. Currently, the phylogeny of rhizobia, as well as of other prokaryotes is based mainly on the analysis of the ribosomal gene 16S. However, there are studies showing that ribosomal genes may occasionally undergo lateral transfer and genetic recombination; therefore, the results would not always reflect correctly the prokaryotic phylogeny. With the aim of minimizing these effects, the MLSA (Multilocus Sequence Analysis) technique was proposed, which uses more than one gene locus, resulting in a more precise analysis. This study used eight strains of R. tropici, including the type strain, and seven type strains of other related species of rhizobia, in order to correlate them taxonomically and phylogenetically. The results obtained by the MLSA analyses were more robust in comparison to the clusters obtained in the individual analysis of each gene. Strains classified as R. tropici were consistently placed in the same great group, but clearly subdivided in two subgroups (I and II), one with three strains of R. tropici type B strains (I) and another with three strains including the reference strain of R. tropici type A (II). Physiological properties were also investigated, comparing the use of 49 carbohydrate sources as substrates; high diversity of profiles was observed, showing consistence with the subgroup division. Altogether, the results have clearly shown that the strains currently classified as R. tropici type A should belong to a new species. Partially financed by CNPq (577933/2008-6; 470162/2009-0). MenosRhizobia are bacteria that live in symbiosis with legumes. In Brazil, the species Rhizobium tropici represents the majority of the population isolated from common bean (Phaseolus vulgaris L.) nodules of field grown plants. Since the description of the R. tropici species in 1991, several strains have shown variability in genetic and physiological properties, splitting the strains into two subgroups, R. tropici type A and type B. Currently, the phylogeny of rhizobia, as well as of other prokaryotes is based mainly on the analysis of the ribosomal gene 16S. However, there are studies showing that ribosomal genes may occasionally undergo lateral transfer and genetic recombination; therefore, the results would not always reflect correctly the prokaryotic phylogeny. With the aim of minimizing these effects, the MLSA (Multilocus Sequence Analysis) technique was proposed, which uses more than one gene locus, resulting in a more precise analysis. This study used eight strains of R. tropici, including the type strain, and seven type strains of other related species of rhizobia, in order to correlate them taxonomically and phylogenetically. The results obtained by the MLSA analyses were more robust in comparison to the clusters obtained in the individual analysis of each gene. Strains classified as R. tropici were consistently placed in the same great group, but clearly subdivided in two subgroups (I and II), one with three strains of R. tropici type B strains (I) and another with thre... Mostrar Tudo |
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Palavras-Chave: |
MLSA. |
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Thesagro: |
Filogenia; Fixação de nitrogênio. |
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Thesaurus Nal: |
Nitrogen fixation; Phylogeny. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/30151/1/ICCC-.12-Renan.pdf
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Marc: |
LEADER 02656nam a2200181 a 4500
001 1877007
005 2011-06-13
008 2010 bl uuuu u00u1 u #d
100 1 $aRIBEIRO, R. A.
245 $aMLSA (Multilocus Sequence Analysis) of brazilian N2-fixing symbionts of common bean (Phaseolus vulgaris L.).
260 $aIn: INTERNATIONAL CONFERENCE ON CULTURE COLLECTIONS, 12., 2010, Florianópolis. Biological Resource Centrers: gateway to biodiversity and services for innovations in biotechnology: proceedings. [S.l]: WFCC$c2010
520 $aRhizobia are bacteria that live in symbiosis with legumes. In Brazil, the species Rhizobium tropici represents the majority of the population isolated from common bean (Phaseolus vulgaris L.) nodules of field grown plants. Since the description of the R. tropici species in 1991, several strains have shown variability in genetic and physiological properties, splitting the strains into two subgroups, R. tropici type A and type B. Currently, the phylogeny of rhizobia, as well as of other prokaryotes is based mainly on the analysis of the ribosomal gene 16S. However, there are studies showing that ribosomal genes may occasionally undergo lateral transfer and genetic recombination; therefore, the results would not always reflect correctly the prokaryotic phylogeny. With the aim of minimizing these effects, the MLSA (Multilocus Sequence Analysis) technique was proposed, which uses more than one gene locus, resulting in a more precise analysis. This study used eight strains of R. tropici, including the type strain, and seven type strains of other related species of rhizobia, in order to correlate them taxonomically and phylogenetically. The results obtained by the MLSA analyses were more robust in comparison to the clusters obtained in the individual analysis of each gene. Strains classified as R. tropici were consistently placed in the same great group, but clearly subdivided in two subgroups (I and II), one with three strains of R. tropici type B strains (I) and another with three strains including the reference strain of R. tropici type A (II). Physiological properties were also investigated, comparing the use of 49 carbohydrate sources as substrates; high diversity of profiles was observed, showing consistence with the subgroup division. Altogether, the results have clearly shown that the strains currently classified as R. tropici type A should belong to a new species. Partially financed by CNPq (577933/2008-6; 470162/2009-0).
650 $aNitrogen fixation
650 $aPhylogeny
650 $aFilogenia
650 $aFixação de nitrogênio
653 $aMLSA
700 1 $aHUNGRIA, M.
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Registro original: |
Embrapa Soja (CNPSO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
05/11/2014 |
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Data da última atualização: |
18/10/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BERENGUER, E.; FERREIRA, J.; GARDNER, T. A.; ARAGÃO, L. E. O. C.; CAMARGO, P. B. de; CERRI, C. E.; DURIGAN, M.; OLIVEIRA JUNIOR, R. C. de; VIEIRA, I. C. G.; BARLOW, J. |
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Afiliação: |
ERIKA BERENGUER, Lancaster University; JOICE NUNES FERREIRA, CPATU; TOBY ALAN GARDNER, University of Cambridge / International Institute for Sustainability / Stockholm Environment Institute; LUIZ EDUARDO OLIVEIRA CRUZ ARAGÃO, University of Exeter / INPE; PLÍNIO BARBOSA DE CAMARGO, CENA/USP; CARLOS EDUARDO CERRI, ESALQ/USP; MARIANA DURIGAN, ESALQ/USP; RAIMUNDO COSME DE OLIVEIRA JUNIOR, CPATU; IMA CELIA GUIMARÃES VIEIRA, MPEG; JOS BARLOW, Lancaster University / MPEG. |
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Título: |
A large-scale field assessment of carbon stocks in human-modified tropical forests. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Global Change Biology, v. 20, n. 12, p. 3713-3726, 2014. |
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DOI: |
10.1111/gcb.12627 |
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Idioma: |
Inglês |
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Conteúdo: |
Tropical rainforests store enormous amounts of carbon, the protection of which represents a vital component of efforts to mitigate global climate change. Currently, tropical forest conservation, science, policies, and climate mitigation actions focus predominantly on reducing carbon emissions from deforestation alone. However, every year vast areas of the humid tropics are disturbed by selective logging, understory fires, and habitat fragmentation. There is an urgent need to understand the effect of such disturbances on carbon stocks, and how stocks in disturbed forests compare to those found in undisturbed primary forests as well as in regenerating secondary forests. Here, we present the results of the largest field study to date on the impacts of human disturbances on above and belowground carbon stocks in tropical forests. Live vegetation, the largest carbon pool, was extremely sensitive to disturbance: forests that experienced both selective logging and understory fires stored, on average, 40% less aboveground carbon than undisturbed forests and were structurally similar to secondary forests. Edge effects also played an important role in explaining variability in aboveground carbon stocks of disturbed forests. Results indicate a potential rapid recovery of the dead wood and litter carbon pools, while soil stocks (0?30 cm) appeared to be resistant to the effects of logging and fire. Carbon loss and subsequent emissions due to human disturbances remain largely unaccounted for in greenhouse gas inventories, but by comparing our estimates of depleted carbon stocks in disturbed forests with Brazilian government assessments of the total forest area annually disturbed in the Amazon, we show that these emissions could represent up to 40% of the carbon loss from deforestation in the region. We conclude that conservation programs aiming to ensure the long-term permanence of forest carbon stocks, such as REDD+, will remain limited in their success unless they effectively avoid degradation as well as deforestation. MenosTropical rainforests store enormous amounts of carbon, the protection of which represents a vital component of efforts to mitigate global climate change. Currently, tropical forest conservation, science, policies, and climate mitigation actions focus predominantly on reducing carbon emissions from deforestation alone. However, every year vast areas of the humid tropics are disturbed by selective logging, understory fires, and habitat fragmentation. There is an urgent need to understand the effect of such disturbances on carbon stocks, and how stocks in disturbed forests compare to those found in undisturbed primary forests as well as in regenerating secondary forests. Here, we present the results of the largest field study to date on the impacts of human disturbances on above and belowground carbon stocks in tropical forests. Live vegetation, the largest carbon pool, was extremely sensitive to disturbance: forests that experienced both selective logging and understory fires stored, on average, 40% less aboveground carbon than undisturbed forests and were structurally similar to secondary forests. Edge effects also played an important role in explaining variability in aboveground carbon stocks of disturbed forests. Results indicate a potential rapid recovery of the dead wood and litter carbon pools, while soil stocks (0?30 cm) appeared to be resistant to the effects of logging and fire. Carbon loss and subsequent emissions due to human disturbances rem... Mostrar Tudo |
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Thesagro: |
Biomassa; Floresta Secundaria; Solo; Vegetação. |
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Thesaurus NAL: |
Amazonia. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02893nga a2200301 a 4500
001 1999228
005 2022-10-18
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1111/gcb.12627$2DOI
100 1 $aBERENGUER, E.
245 $aA large-scale field assessment of carbon stocks in human-modified tropical forests.
260 $c2014
520 $aTropical rainforests store enormous amounts of carbon, the protection of which represents a vital component of efforts to mitigate global climate change. Currently, tropical forest conservation, science, policies, and climate mitigation actions focus predominantly on reducing carbon emissions from deforestation alone. However, every year vast areas of the humid tropics are disturbed by selective logging, understory fires, and habitat fragmentation. There is an urgent need to understand the effect of such disturbances on carbon stocks, and how stocks in disturbed forests compare to those found in undisturbed primary forests as well as in regenerating secondary forests. Here, we present the results of the largest field study to date on the impacts of human disturbances on above and belowground carbon stocks in tropical forests. Live vegetation, the largest carbon pool, was extremely sensitive to disturbance: forests that experienced both selective logging and understory fires stored, on average, 40% less aboveground carbon than undisturbed forests and were structurally similar to secondary forests. Edge effects also played an important role in explaining variability in aboveground carbon stocks of disturbed forests. Results indicate a potential rapid recovery of the dead wood and litter carbon pools, while soil stocks (0?30 cm) appeared to be resistant to the effects of logging and fire. Carbon loss and subsequent emissions due to human disturbances remain largely unaccounted for in greenhouse gas inventories, but by comparing our estimates of depleted carbon stocks in disturbed forests with Brazilian government assessments of the total forest area annually disturbed in the Amazon, we show that these emissions could represent up to 40% of the carbon loss from deforestation in the region. We conclude that conservation programs aiming to ensure the long-term permanence of forest carbon stocks, such as REDD+, will remain limited in their success unless they effectively avoid degradation as well as deforestation.
650 $aAmazonia
650 $aBiomassa
650 $aFloresta Secundaria
650 $aSolo
650 $aVegetação
700 1 $aFERREIRA, J.
700 1 $aGARDNER, T. A.
700 1 $aARAGÃO, L. E. O. C.
700 1 $aCAMARGO, P. B. de
700 1 $aCERRI, C. E.
700 1 $aDURIGAN, M.
700 1 $aOLIVEIRA JUNIOR, R. C. de
700 1 $aVIEIRA, I. C. G.
700 1 $aBARLOW, J.
773 $tGlobal Change Biology$gv. 20, n. 12, p. 3713-3726, 2014.
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