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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
30/06/2010 |
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Data da última atualização: |
18/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. |
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Afiliação: |
CHRISTINA RAMIRES FERREIRA, UNESP-JABOTICABAL; JORG PATRICK BURGSTALLER, UNIVERSTIY OF VETERINARY MEDICINE - VIENNA; FELIPE PERECIN, UNESP - JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP - JABOTICABAL; MARCOS ROBERTO CHIARATTI, USP - PIRASSUNUNGA; SIMONE CRISTINA MÉO, CPPSE; MATHIAS MULLER, UNIVERSITY OF VETERINARY MEDICINE - VIENNA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL - QUEBEC; FLÁVIO VIEIRA MEIRELLES, USP - PIRASSUNUNGA; RALF STEINBORN, UNIVERSITY OF VETERINARY MEDICINE - VIENNA. |
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Título: |
Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010. |
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DOI: |
https://doi.org/10.1095/biolreprod.109.080564 |
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Idioma: |
Inglês |
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Conteúdo: |
Ooplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype. |
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Palavras-Chave: |
Bovine; Developmental biology; Embryo; Gamete biology; Ooplasmic. |
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Thesagro: |
DNA. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/28805/1/PROCISCMN2010.00002.pdf
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Marc: |
LEADER 02370naa a2200313 a 4500
001 1856388
005 2022-11-18
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1095/biolreprod.109.080564$2DOI
100 1 $aFERREIRA, C. R.
245 $aPronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line.$h[electronic resource]
260 $c2010
520 $aOoplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype.
650 $aDNA
653 $aBovine
653 $aDevelopmental biology
653 $aEmbryo
653 $aGamete biology
653 $aOoplasmic
700 1 $aBURGSTALLER, J. P.
700 1 $aPERECIN, F.
700 1 $aGARCIA, J. M.
700 1 $aCHIARATTI, M. R.
700 1 $aMÉO, S. C.
700 1 $aMULLER, M.
700 1 $aSMITH, L. C.
700 1 $aMEIRELLES, F. V.
700 1 $aSTEINBORN, R.
773 $tBiology of Reproduction$gv. 82, n. 03, p. 563-571, mar. 2010.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
10/10/2013 |
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Data da última atualização: |
16/06/2017 |
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Autoria: |
NUNES, C. da s.; MORAES, G.; FABRIZZI, F.; HACKBARTH, A.; ARBELÁEZ-ROJAS, G. A. |
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Afiliação: |
CLEUJOSÍ DA SILVA NUNES, Universidade Federal de São Carlos, Departamento de Genética e Evolução, Laboratório de Bioquímica Adaptativa; GILBERTO MORAES, Universidade Federal de São Carlos, Departamento de Genética e Evolução, Laboratório de Bioquímica Adaptativa; FERNANDO FABRIZZI, Universidade Federal de São Carlos, Departamento de Genética e Evolução, Laboratório de Bioquímica Adaptativa; ARACELI HACKBARTH, Universidade Federal de São Carlos, Departamento de Genética e Evolução, Laboratório de Bioquímica Adaptativa; GUSTAVO ALBERTO ARBELÁEZ-ROJAS, Universidade Federal de São Carlos, Departamento de Genética e Evolução, Laboratório de Bioquímica Adaptativa. |
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Título: |
Growth and hematology of pacu subjected to sustained swimming and fed different protein levels. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 6, p. 645-650, jun. 2013. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Crescimento e hematologia de pacu submetido a natação sustentada e alimentado com diferentes níveis proteicos. |
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Conteúdo: |
The objective of this work was to evaluate the effect of sustained swimming and dietary protein levels on growth and hematological responses of juvenile pacu (Piaractus mesopotamicus). A completely randomized design was used in a 3x2 factorial arrangement, with three levels of dietary protein (24, 28, and 32% crude protein), two rearing conditions (sustained swimming or motionless water), and 15 replicates. Fish were subjected to sustained swimming at the velocity of two body lengths per second (2 BL-1), for 45 days. The level of dietary protein and the swimming conditions affected the performance, growth, and hematological profile of pacu. Swimming conditions influenced nutritional factors, increasing daily weight gain, specific growth rate, number of erythrocytes, mean corpuscular volume, and mean corpuscular hemoglobin. Fish under sustained swimming and fed with 24% crude protein showed better growth performance, with higher specific growth rate (4.11±0.88) and higher daily weight gain (2.19±0.47 g per day). Sustained swimming can increase the productive performance of pacu and simultaneously reduce dietary protein levels. |
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Thesagro: |
Fator de crescimento; Nutrição animal; Pacu. |
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Thesaurus NAL: |
Animal growth; Erythropoiesis; Piaractus mesopotamicus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/90750/1/growth-and-hematology-of-pacu.pdf
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Marc: |
LEADER 02022naa a2200253 a 4500
001 1968247
005 2017-06-16
008 2013 bl uuuu u00u1 u #d
100 1 $aNUNES, C. da s.
245 $aGrowth and hematology of pacu subjected to sustained swimming and fed different protein levels.
260 $c2013
500 $aTítulo em português: Crescimento e hematologia de pacu submetido a natação sustentada e alimentado com diferentes níveis proteicos.
520 $aThe objective of this work was to evaluate the effect of sustained swimming and dietary protein levels on growth and hematological responses of juvenile pacu (Piaractus mesopotamicus). A completely randomized design was used in a 3x2 factorial arrangement, with three levels of dietary protein (24, 28, and 32% crude protein), two rearing conditions (sustained swimming or motionless water), and 15 replicates. Fish were subjected to sustained swimming at the velocity of two body lengths per second (2 BL-1), for 45 days. The level of dietary protein and the swimming conditions affected the performance, growth, and hematological profile of pacu. Swimming conditions influenced nutritional factors, increasing daily weight gain, specific growth rate, number of erythrocytes, mean corpuscular volume, and mean corpuscular hemoglobin. Fish under sustained swimming and fed with 24% crude protein showed better growth performance, with higher specific growth rate (4.11±0.88) and higher daily weight gain (2.19±0.47 g per day). Sustained swimming can increase the productive performance of pacu and simultaneously reduce dietary protein levels.
650 $aAnimal growth
650 $aErythropoiesis
650 $aPiaractus mesopotamicus
650 $aFator de crescimento
650 $aNutrição animal
650 $aPacu
700 1 $aMORAES, G.
700 1 $aFABRIZZI, F.
700 1 $aHACKBARTH, A.
700 1 $aARBELÁEZ-ROJAS, G. A.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 48, n. 6, p. 645-650, jun. 2013.
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Embrapa Unidades Centrais (AI-SEDE) |
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