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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
12/07/2023 |
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Autoria: |
MACKENZIE, C. D.; JUNGERY, M.; TAYLOR, P. M.; OGILVE, B. N. |
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Título: |
The in vitro interaction of eosinophils, neutrophils, macrophages and mast cells with nematode surfaces in the presence of complement or antibodies. |
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Ano de publicação: |
1981 |
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Fonte/Imprenta: |
Journal Pathology, v. 133, n. 2, p. 161-175, 1981. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The adherence in vitro of leucocytes to the surface of various stages in the life cycle of T. spiralis and N. brasiliensis in the presence of serum was examined. Considerable differences were observed in the behaviour of mast cells, eosinophils, neutrophils and macrophages in this interaction. Mast cells adhered for a short time, did not flatten onto the surface and did not degranulate. Adherence ceased after 4-6 hr. Eosinophils adhered within minutes to the surface or worms, flattened and degranulated; only their cytoplasmic remnants could be seen on the worms' surface after 24 hr in culture. In contrast, only a small area of the cytoplasmic membrane of neutrophils flattened on the surface of the worms and adherence ceased after 2-24 hr. The NBT conversion reaction showed a positive deposit at the interface between neutrophils and parasites during neutrophil adherence. This deposit remained as "foot prints" on the surface of the nematodes following neutrophil detachment. This positive NBT reaction occurred only with neutrophils and not with eosinophils, mast cells or macrophages. Macrophages adhered permanently to the surface of these worms, they did not flatten and retained their integrity. Under the light microscope the cytoplasmic inclusions appeared to decrease in size during culture. Electron microscopy revealed the presence of fewer granules and an increased number of vacuoles in later cultures of macrophages. These findings are discussed in relationship to the immunopathology of nematode infection in vivo MenosAbstract: The adherence in vitro of leucocytes to the surface of various stages in the life cycle of T. spiralis and N. brasiliensis in the presence of serum was examined. Considerable differences were observed in the behaviour of mast cells, eosinophils, neutrophils and macrophages in this interaction. Mast cells adhered for a short time, did not flatten onto the surface and did not degranulate. Adherence ceased after 4-6 hr. Eosinophils adhered within minutes to the surface or worms, flattened and degranulated; only their cytoplasmic remnants could be seen on the worms' surface after 24 hr in culture. In contrast, only a small area of the cytoplasmic membrane of neutrophils flattened on the surface of the worms and adherence ceased after 2-24 hr. The NBT conversion reaction showed a positive deposit at the interface between neutrophils and parasites during neutrophil adherence. This deposit remained as "foot prints" on the surface of the nematodes following neutrophil detachment. This positive NBT reaction occurred only with neutrophils and not with eosinophils, mast cells or macrophages. Macrophages adhered permanently to the surface of these worms, they did not flatten and retained their integrity. Under the light microscope the cytoplasmic inclusions appeared to decrease in size during culture. Electron microscopy revealed the presence of fewer granules and an increased number of vacuoles in later cultures of macrophages. These findings are discussed in relationship to ... Mostrar Tudo |
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Palavras-Chave: |
Complement system proteins; Nematodeo. |
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Thesagro: |
Anticorpo; Imunologia; Mamífero; Parasitologia. |
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Thesaurus Nal: |
Antibodies; Cell adhesion; Eosinophils; Leukocytes; Macrophages; Mast cells; Nematoda; Nitroblue tetrazolium; Rats; Trichinella. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02541naa a2200349 a 4500
001 1521898
005 2023-07-12
008 1981 bl uuuu u00u1 u #d
100 1 $aMACKENZIE, C. D.
245 $aThe in vitro interaction of eosinophils, neutrophils, macrophages and mast cells with nematode surfaces in the presence of complement or antibodies.$h[electronic resource]
260 $c1981
520 $aAbstract: The adherence in vitro of leucocytes to the surface of various stages in the life cycle of T. spiralis and N. brasiliensis in the presence of serum was examined. Considerable differences were observed in the behaviour of mast cells, eosinophils, neutrophils and macrophages in this interaction. Mast cells adhered for a short time, did not flatten onto the surface and did not degranulate. Adherence ceased after 4-6 hr. Eosinophils adhered within minutes to the surface or worms, flattened and degranulated; only their cytoplasmic remnants could be seen on the worms' surface after 24 hr in culture. In contrast, only a small area of the cytoplasmic membrane of neutrophils flattened on the surface of the worms and adherence ceased after 2-24 hr. The NBT conversion reaction showed a positive deposit at the interface between neutrophils and parasites during neutrophil adherence. This deposit remained as "foot prints" on the surface of the nematodes following neutrophil detachment. This positive NBT reaction occurred only with neutrophils and not with eosinophils, mast cells or macrophages. Macrophages adhered permanently to the surface of these worms, they did not flatten and retained their integrity. Under the light microscope the cytoplasmic inclusions appeared to decrease in size during culture. Electron microscopy revealed the presence of fewer granules and an increased number of vacuoles in later cultures of macrophages. These findings are discussed in relationship to the immunopathology of nematode infection in vivo
650 $aAntibodies
650 $aCell adhesion
650 $aEosinophils
650 $aLeukocytes
650 $aMacrophages
650 $aMast cells
650 $aNematoda
650 $aNitroblue tetrazolium
650 $aRats
650 $aTrichinella
650 $aAnticorpo
650 $aImunologia
650 $aMamífero
650 $aParasitologia
653 $aComplement system proteins
653 $aNematodeo
700 1 $aJUNGERY, M.
700 1 $aTAYLOR, P. M.
700 1 $aOGILVE, B. N.
773 $tJournal Pathology$gv. 133, n. 2, p. 161-175, 1981.
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| 6. |  | AZEVEDO, R. A.; MACHADO, F. S.; CAMPOS, M. M.; FURINI, P. M.; RUFINO, S. R.; PEREIRA, L. G. R.; TOMICH, T. R.; COELHO, S. G. The effects of increasing amounts of milk replacer powder added to whole milk on feed intake and performance in dairy heifers. Journal of Dairy Science, v. 99, n. 10, p. 8018-8027, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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