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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
07/12/2023 |
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Autoria: |
RANA, A. P. S.; MAJUMDER, G. C. A. |
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Título: |
A two-phase polymer method for isolation of maturing goat sperm plasmamembrane. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Preparative Biochemistry, v. 19, n. 2, p. 167-173, 1989. |
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DOI: |
10.1080/10826068908544906 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: An aqueous two-phase polymer method originally developed for the isolation of plasma membrane from mature goat epididymal spermatozoa (Rana, A.P.S. and Majumder, G.C., Prep. Biochem., 17, 261, 1987) has been found to be unsuitable for the maturing spermatozoa derived from caput and corpus epididymides because of significant contamination of the isolated membrane with intact cells. A modified method has been developed by manipulating the centrifugal force (required for membrane sedimentation) for the isolation of maturing sperm plasma membrane of high yield (approximately 55%) and purity as judged by marker enzyme assays and phase contrast and electron microscopic analyses. The method consists of treatment of intact spermatozoa with 1.25 mM EDTA, dispersion of these cells to a two-phase polymer system comprising 5.5% 252-Kd dextran and 4.2% 20-Kd polyethylene glycol compound and subsequent centrifugation at 12,000 X g for 30 min when the two phases separate out and membranes sediment at the interphase. The repeatation of the two-phase fractionation step yielded greater purity of the plasma membrane. |
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Palavras-Chave: |
Sperm maturation. |
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Thesagro: |
Bioquímica; Caprino; Reprodução; Sêmen. |
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Thesaurus Nal: |
Cell membranes; Centrifugation; Goats; Reproduction; Spermatozoa; Ultracentrifugation; Ultrastructure. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01912naa a2200289 a 4500
001 1520994
005 2023-12-07
008 1989 bl uuuu u00u1 u #d
024 7 $a10.1080/10826068908544906$2DOI
100 1 $aRANA, A. P. S.
245 $aA two-phase polymer method for isolation of maturing goat sperm plasmamembrane.
260 $c1989
520 $aAbstract: An aqueous two-phase polymer method originally developed for the isolation of plasma membrane from mature goat epididymal spermatozoa (Rana, A.P.S. and Majumder, G.C., Prep. Biochem., 17, 261, 1987) has been found to be unsuitable for the maturing spermatozoa derived from caput and corpus epididymides because of significant contamination of the isolated membrane with intact cells. A modified method has been developed by manipulating the centrifugal force (required for membrane sedimentation) for the isolation of maturing sperm plasma membrane of high yield (approximately 55%) and purity as judged by marker enzyme assays and phase contrast and electron microscopic analyses. The method consists of treatment of intact spermatozoa with 1.25 mM EDTA, dispersion of these cells to a two-phase polymer system comprising 5.5% 252-Kd dextran and 4.2% 20-Kd polyethylene glycol compound and subsequent centrifugation at 12,000 X g for 30 min when the two phases separate out and membranes sediment at the interphase. The repeatation of the two-phase fractionation step yielded greater purity of the plasma membrane.
650 $aCell membranes
650 $aCentrifugation
650 $aGoats
650 $aReproduction
650 $aSpermatozoa
650 $aUltracentrifugation
650 $aUltrastructure
650 $aBioquímica
650 $aCaprino
650 $aReprodução
650 $aSêmen
653 $aSperm maturation
700 1 $aMAJUMDER, G. C. A.
773 $tPreparative Biochemistry$gv. 19, n. 2, p. 167-173, 1989.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 12 | |
| 2. |  | SILVA, S. de O. e; SHEPHERD, K.; DANTAS, J. L. L.; ALVES, E. J.; BORGES, A. L.; FANCELLI, M.; OLIVEIRA, S. L. de; OLIVEIRA, M. de A. Avanços do programa de pesquisa em Musa no CNPMF, EMBRAPA, Brasil Cruz das Almas: EMBRAPA-CNPMF, 1995. 37 p. (EMBRAPA-CNPMF. Documentos, 65)| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | CARVALHO, H. W. L. de; LEAL, M. de L. da S.; CARDOSO, M. J.; SANTOS, M. X. dos; ALBUQUERQUE, M. M. de; SANTOS, D. M. dos; TABOSA, J. N.; CARVALHO, B. C. L. de; LIRA, M. A.; SILVA, I. O.; MARQUES, H. da S.; SAMPAIO, G. V.; DOURADO, V. V. Cultivares de milho para o Nordeste brasileiro: ensaios realizados no ano agrícola de 1998. Aracaju: CPATC, 1999. 6 p. (Embrapa CPATC. Comunicado Técnico, 30).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Tabuleiros Costeiros. |
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| Registros recuperados : 12 | |
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| Registros recuperados : 1 | |
| 1. | | 6050009, IRISH VETERINARY JOURNAL, Irish Veterinary Association, Dublin-Irlanda
Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Caprinos e Ovinos; Embrapa Clima Temperado; Embrapa Gado de Corte; Embrapa Gado de Leite; Embrapa Semiárido; Embrapa Suínos e Aves. | |
| Registros recuperados : 1 | |
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