Registro Completo
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Biblioteca(s): |
Embrapa Semiárido; Embrapa Uva e Vinho. |
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Data corrente: |
29/11/2019 |
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Data da última atualização: |
10/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VILLELA, J. G. A.; RITSCHEL, P. S.; BARBOSA, M. A. G.; BACCIN, J. M. S.; ROSSATO, M.; MAIA, J. D. G.; FERREIRA, M. A. S. V. |
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Afiliação: |
João Gilberto A. Villela; PATRICIA SILVA RITSCHEL, CNPUV; MARIA ANGELICA GUIMARAES BARBOSA, CPATSA; Kétini M. S. Baccin; Mauricio Rossato; JOAO DIMAS GARCIA MAIA, CNPUV; Marisa A. S. V. Ferreira. |
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Título: |
Detection of Xanthomonas citri pv. viticola on grapevine by real-time PCR and BIO-PCR using primers designed from pathogenicity and xanthomonadin gene sequences. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
European Journal of Plant Pathology, v. 155, n. 2, p. 445-459, 2019. |
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DOI: |
10.1007/s10658-019-01779-y |
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Idioma: |
Inglês |
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Conteúdo: |
Grapevine bacterial canker caused by Xanthomonas citri pv. viticola (X. campestris pv. viticola) (Xcv), was detected in Brazil in 1998 and is currently regarded as a quarantine disease with limited distribution in the country. To improve sensitivity and speed in the detection of Xcv in asymptomatic grapevines, two pairs of primers were designed, targeting sequences of a pathogenicity gene (hrpB) and the xanthomonadin coding cluster. Both pairs were tested in conventional PCR (cPCR) and real-time PCR (qPCR) formats. Primers targeting the hrpB gene showed cross reactions with other Xanthomonas spp. but were effective for use in both cPCR and qPCR, whereas primers for the xanthomonadin gene were highly specific for Xcv but showed low efficiency in qPCR. Enrichment of plant extracts in semi-selective medium before qPCR allowed a significant increase in sensitivity when compared to total DNA extraction, making it possible to detect as low as 101 CFU ml-1. Under natural infection conditions, symptomatic and asymptomatic grapevines were tested by qPCR with hrpB primers and cPCR with xanthomonadin primers. In both cases, plant extracts were enriched for 36-72h. Xcv was detected in all symptomatic samples by qPCR and the result was confirmed by cPCR. For the asymptomatic samples, Xcv was detected in 93.4% with qPCR and in 89.5% with cPCR. These two methods offer advantages in terms of sensitivity and specificity, and they could be useful in quarantine programs, certification of grapevine propagating material and detection of inoculum sources in alternative hosts, contributing to the prevention of pathogen spread to disease-free areas. MenosGrapevine bacterial canker caused by Xanthomonas citri pv. viticola (X. campestris pv. viticola) (Xcv), was detected in Brazil in 1998 and is currently regarded as a quarantine disease with limited distribution in the country. To improve sensitivity and speed in the detection of Xcv in asymptomatic grapevines, two pairs of primers were designed, targeting sequences of a pathogenicity gene (hrpB) and the xanthomonadin coding cluster. Both pairs were tested in conventional PCR (cPCR) and real-time PCR (qPCR) formats. Primers targeting the hrpB gene showed cross reactions with other Xanthomonas spp. but were effective for use in both cPCR and qPCR, whereas primers for the xanthomonadin gene were highly specific for Xcv but showed low efficiency in qPCR. Enrichment of plant extracts in semi-selective medium before qPCR allowed a significant increase in sensitivity when compared to total DNA extraction, making it possible to detect as low as 101 CFU ml-1. Under natural infection conditions, symptomatic and asymptomatic grapevines were tested by qPCR with hrpB primers and cPCR with xanthomonadin primers. In both cases, plant extracts were enriched for 36-72h. Xcv was detected in all symptomatic samples by qPCR and the result was confirmed by cPCR. For the asymptomatic samples, Xcv was detected in 93.4% with qPCR and in 89.5% with cPCR. These two methods offer advantages in terms of sensitivity and specificity, and they could be useful in quarantine programs, certification of grape... Mostrar Tudo |
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Palavras-Chave: |
QPCR de diagnóstico baseado em PCR; Videira. |
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Thesagro: |
Cancro Bacteriano; Doença; Doença Fúngica; Uva; Xanthomonas Citri. |
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Thesaurus NAL: |
Bacterial canker; Grapes. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/206475/1/Detection-of-Xanthomonas-citri-pv-viticola-2019.pdf
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Marc: |
LEADER 02654naa a2200313 a 4500
001 2116344
005 2019-12-10
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s10658-019-01779-y$2DOI
100 1 $aVILLELA, J. G. A.
245 $aDetection of Xanthomonas citri pv. viticola on grapevine by real-time PCR and BIO-PCR using primers designed from pathogenicity and xanthomonadin gene sequences.$h[electronic resource]
260 $c2019
520 $aGrapevine bacterial canker caused by Xanthomonas citri pv. viticola (X. campestris pv. viticola) (Xcv), was detected in Brazil in 1998 and is currently regarded as a quarantine disease with limited distribution in the country. To improve sensitivity and speed in the detection of Xcv in asymptomatic grapevines, two pairs of primers were designed, targeting sequences of a pathogenicity gene (hrpB) and the xanthomonadin coding cluster. Both pairs were tested in conventional PCR (cPCR) and real-time PCR (qPCR) formats. Primers targeting the hrpB gene showed cross reactions with other Xanthomonas spp. but were effective for use in both cPCR and qPCR, whereas primers for the xanthomonadin gene were highly specific for Xcv but showed low efficiency in qPCR. Enrichment of plant extracts in semi-selective medium before qPCR allowed a significant increase in sensitivity when compared to total DNA extraction, making it possible to detect as low as 101 CFU ml-1. Under natural infection conditions, symptomatic and asymptomatic grapevines were tested by qPCR with hrpB primers and cPCR with xanthomonadin primers. In both cases, plant extracts were enriched for 36-72h. Xcv was detected in all symptomatic samples by qPCR and the result was confirmed by cPCR. For the asymptomatic samples, Xcv was detected in 93.4% with qPCR and in 89.5% with cPCR. These two methods offer advantages in terms of sensitivity and specificity, and they could be useful in quarantine programs, certification of grapevine propagating material and detection of inoculum sources in alternative hosts, contributing to the prevention of pathogen spread to disease-free areas.
650 $aBacterial canker
650 $aGrapes
650 $aCancro Bacteriano
650 $aDoença
650 $aDoença Fúngica
650 $aUva
650 $aXanthomonas Citri
653 $aQPCR de diagnóstico baseado em PCR
653 $aVideira
700 1 $aRITSCHEL, P. S.
700 1 $aBARBOSA, M. A. G.
700 1 $aBACCIN, J. M. S.
700 1 $aROSSATO, M.
700 1 $aMAIA, J. D. G.
700 1 $aFERREIRA, M. A. S. V.
773 $tEuropean Journal of Plant Pathology$gv. 155, n. 2, p. 445-459, 2019.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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