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1. | | DE CÉSARO, A.; SOUZA, P. V. D. de; SANTOS, H. P. dos; FELIPPETO, J. Caracterização de desenvolvimento vegetativo e taxa fotossintética em videiras inffestadas por pérola-da-terra. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 5., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 1., 2007, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2007. p. 54. (Embrapa Uva e Vinho. Documentos, 63). Resumo. Biblioteca(s): Embrapa Uva e Vinho. |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
02/10/2020 |
Data da última atualização: |
07/10/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SANTANA, M. F.; ZAMBOLIM, E. M.; CAIXETA, E. T.; ZAMBOLIM, L. |
Afiliação: |
Mateus F. Santana, Universidade Federal de Viçosa; Eunize M. Zambolim, Universidade Federal de Viçosa; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa; Laércio Zambolim, Universidade Federal de Viçosa. |
Título: |
Population genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Tropical Plant Pathology, v. 43, n. 5 , p. 473-476, 2018. |
DOI: |
https://doi.org/10.1007/s40858-018-0246-9 |
Idioma: |
Inglês |
Conteúdo: |
The biotrophic fungus Hemileia vastatrix Berk & Broome is the most destructive coffee pathogen in Brazil. Better understanding of the population genetics of H. vastatrix would provide important insights into its biology, epidemiology, and evolutionary potential. The aim of the present study was to assess the genetic diversity and population structure of H. vastatrix in Minas Gerais (Brazil) using ribosomal DNA (rDNA) sequences. The analyzes were performed by sequencing the internal transcribed spacers ITS1 and ITS2, and the 5.8S gene from 15 H. vastatrix populations. Of the 82 sequences obtained, 68 ribotypes were found, as defined by 108 nucleotide substitutions and five indels. Of the 68 ribotypes, 64 were exclusively found in one population. Analysis of molecular variance (AMOVA) and FST fixation index indicated moderate genetic differentiation among field populations, which were divided according to geographic origin. In conclusion, analysis of the nuclear ITS1?5.8S-ITS2 rDNA sequence diversity in the H. vastatrix population of Minas Gerais revealed that most ribotypes are restricted to a single population and that there exists greater genetic diversity within than among field populations. |
Palavras-Chave: |
Coffee rust; ITS polymorphism. |
Thesagro: |
Coffea Arábica. |
Thesaurus NAL: |
Population structure. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216380/1/Santana-et-al-2018-1.pdf
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Marc: |
LEADER 01897naa a2200217 a 4500 001 2125253 005 2020-10-07 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s40858-018-0246-9$2DOI 100 1 $aSANTANA, M. F. 245 $aPopulation genetic structure of the coffee pathogen Hemileia vastatrix in Minas Gerais, Brazil.$h[electronic resource] 260 $c2018 520 $aThe biotrophic fungus Hemileia vastatrix Berk & Broome is the most destructive coffee pathogen in Brazil. Better understanding of the population genetics of H. vastatrix would provide important insights into its biology, epidemiology, and evolutionary potential. The aim of the present study was to assess the genetic diversity and population structure of H. vastatrix in Minas Gerais (Brazil) using ribosomal DNA (rDNA) sequences. The analyzes were performed by sequencing the internal transcribed spacers ITS1 and ITS2, and the 5.8S gene from 15 H. vastatrix populations. Of the 82 sequences obtained, 68 ribotypes were found, as defined by 108 nucleotide substitutions and five indels. Of the 68 ribotypes, 64 were exclusively found in one population. Analysis of molecular variance (AMOVA) and FST fixation index indicated moderate genetic differentiation among field populations, which were divided according to geographic origin. In conclusion, analysis of the nuclear ITS1?5.8S-ITS2 rDNA sequence diversity in the H. vastatrix population of Minas Gerais revealed that most ribotypes are restricted to a single population and that there exists greater genetic diversity within than among field populations. 650 $aPopulation structure 650 $aCoffea Arábica 653 $aCoffee rust 653 $aITS polymorphism 700 1 $aZAMBOLIM, E. M. 700 1 $aCAIXETA, E. T. 700 1 $aZAMBOLIM, L. 773 $tTropical Plant Pathology$gv. 43, n. 5 , p. 473-476, 2018.
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