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| Registros recuperados : 6 | |
| 2. |  | PEREIRA, M. C. T.; GOMES, R. A.; DANTAS, B. F.; DANTAS, E. W. Banco de sementes de macrófitas aquáticas de lagoas intermitentes do Semiárido. Informativo ABRATES, Brasília, DF, v. 24, n. 3, p. 101, dez. 2014. Edição dos Resumos do V Workshop de Tecnologia e Fisiologia de Sementes e Mudas, Petrolina, dez. 2014.| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 6 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja; Embrapa Trigo. |
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Data corrente: |
27/09/2023 |
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Data da última atualização: |
27/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
SILVA. D. A. da; CARPENTIERI-PIPOLO, V.; BARRETO, T.; ABDELNOOR, R. V.; MARIN, S. R. R.; CARRAO-PANIZZI, M. C. |
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Afiliação: |
Instituto Agronômico Campinas; VALERIA CARPENTIERI PIPOLO, CNPT; Syngenta Proteção de Cultivos LTDA; RICARDO VILELA ABDELNOOR, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; MERCEDES CONCÓRDIA CARRÃO-PANIZZI. |
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Título: |
Genetic removal of trypsin inhibitor and lipoxygenase isozymes form soybean seeds (Glycine max) by simple sequence repeat marker assisted selection. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 3, GMR19145, 2023. |
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DOI: |
https://doi.org/10.4238/gmr19145 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: unitz trypsin inhibitor (KTI), which affects protein digestibility and lipoxygenase isozymes, responsible for the off-flavor associated with soy-based foods, are two undesirable factors present in soybean seeds. These unpleasant factors are usually inactivated by heat treatment. However, heat treatment does not completely eliminate these factors; in addition it may decrease protein solubility and may incur extra energy costs. Genetic elimination of these factors could be an alternative to heat treatment. This study aimed to select soybean lines free of KTI and lipoxygenase isozymes in the seeds. The population under study was obtained by crossing the BRS 213 cultivar, which shows low lipoxygenase activity, with BRS 155, a KTI lacking cultivar. F2:3 hybrid populations were selected and analyzed using DNA markers for the identification of recessive alleles that encode the absence of KTI and the three lipoxygenase enzymes (LOX1, LOX2 and LOX3). F2:3 segregating populations were successfully identified with the KTI specific marker with 100% efficiency. However, the KTi/kti-gene-specific marker does not allow for the identification of heterozygous and homozygous genotypes with dominant KTi alleles; the the simple sequence repeat (SSR) markers Satt228 and Satt409, which bind tightly to the KTi locus, were much more suitable diagnostic marker for screening plants in segregating populations. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3.00 and 2.77 cM, respectively. The SSR markers used in this study could be efficiently used in marker assisted selection in a breeding program aimed at improving soybean seed quality. MenosAbstract: unitz trypsin inhibitor (KTI), which affects protein digestibility and lipoxygenase isozymes, responsible for the off-flavor associated with soy-based foods, are two undesirable factors present in soybean seeds. These unpleasant factors are usually inactivated by heat treatment. However, heat treatment does not completely eliminate these factors; in addition it may decrease protein solubility and may incur extra energy costs. Genetic elimination of these factors could be an alternative to heat treatment. This study aimed to select soybean lines free of KTI and lipoxygenase isozymes in the seeds. The population under study was obtained by crossing the BRS 213 cultivar, which shows low lipoxygenase activity, with BRS 155, a KTI lacking cultivar. F2:3 hybrid populations were selected and analyzed using DNA markers for the identification of recessive alleles that encode the absence of KTI and the three lipoxygenase enzymes (LOX1, LOX2 and LOX3). F2:3 segregating populations were successfully identified with the KTI specific marker with 100% efficiency. However, the KTi/kti-gene-specific marker does not allow for the identification of heterozygous and homozygous genotypes with dominant KTi alleles; the the simple sequence repeat (SSR) markers Satt228 and Satt409, which bind tightly to the KTi locus, were much more suitable diagnostic marker for screening plants in segregating populations. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in th... Mostrar Tudo |
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Palavras-Chave: |
Inibidor de tripsina; Kunitz Isoenzimas lipoxigenase; Kunitz trypsin inhibitor; Lipoxygenase isozymes; Molecular markers. |
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Thesagro: |
Controle de Qualidade; Glycine Max; Marcador Molecular; Semente; Soja. |
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Thesaurus NAL: |
Seeds; Soybeans. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156934/1/genetic-removal-trypsin-inhibitor-and-lipoxygenase-isozymes-form-soybean-seeds-glycine-max.pdf
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Marc: |
LEADER 02807naa a2200337 a 4500
001 2156934
005 2023-09-27
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/gmr19145$2DOI
100 1 $aSILVA. D. A. da
245 $aGenetic removal of trypsin inhibitor and lipoxygenase isozymes form soybean seeds (Glycine max) by simple sequence repeat marker assisted selection.$h[electronic resource]
260 $c2023
520 $aAbstract: unitz trypsin inhibitor (KTI), which affects protein digestibility and lipoxygenase isozymes, responsible for the off-flavor associated with soy-based foods, are two undesirable factors present in soybean seeds. These unpleasant factors are usually inactivated by heat treatment. However, heat treatment does not completely eliminate these factors; in addition it may decrease protein solubility and may incur extra energy costs. Genetic elimination of these factors could be an alternative to heat treatment. This study aimed to select soybean lines free of KTI and lipoxygenase isozymes in the seeds. The population under study was obtained by crossing the BRS 213 cultivar, which shows low lipoxygenase activity, with BRS 155, a KTI lacking cultivar. F2:3 hybrid populations were selected and analyzed using DNA markers for the identification of recessive alleles that encode the absence of KTI and the three lipoxygenase enzymes (LOX1, LOX2 and LOX3). F2:3 segregating populations were successfully identified with the KTI specific marker with 100% efficiency. However, the KTi/kti-gene-specific marker does not allow for the identification of heterozygous and homozygous genotypes with dominant KTi alleles; the the simple sequence repeat (SSR) markers Satt228 and Satt409, which bind tightly to the KTi locus, were much more suitable diagnostic marker for screening plants in segregating populations. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3.00 and 2.77 cM, respectively. The SSR markers used in this study could be efficiently used in marker assisted selection in a breeding program aimed at improving soybean seed quality.
650 $aSeeds
650 $aSoybeans
650 $aControle de Qualidade
650 $aGlycine Max
650 $aMarcador Molecular
650 $aSemente
650 $aSoja
653 $aInibidor de tripsina
653 $aKunitz Isoenzimas lipoxigenase
653 $aKunitz trypsin inhibitor
653 $aLipoxygenase isozymes
653 $aMolecular markers
700 1 $aCARPENTIERI-PIPOLO, V.
700 1 $aBARRETO, T.
700 1 $aABDELNOOR, R. V.
700 1 $aMARIN, S. R. R.
700 1 $aCARRAO-PANIZZI, M. C.
773 $tGenetics and Molecular Research$gv. 22, n. 3, GMR19145, 2023.
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Embrapa Trigo (CNPT) |
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