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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/11/2004 |
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Data da última atualização: |
23/11/2015 |
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Autoria: |
CARVALHO, C. H. S.; ZEHR, U. B.; GUNARATNA, N.; ANDERSON, J.; KONONOWICZ, H. H.; HODGES, T. K.; AXTELL, J. D. |
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Afiliação: |
CARLOS HENRIQUE S DE CARVALHO, Embrapa Milho e Sorgo; Usha B. Zehr, Purdue University; Nilupa Gunaratna, Purdue University; Joseph Anderson, Purdue University; Halina H. Kononowicz, Purdue University; Thomas K. Hodges, Purdue University; John D. Axtell, Purdue University. |
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Título: |
Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 27, n. 2, p. 259-269, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection. MenosThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculu... Mostrar Tudo |
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Thesagro: |
Sorgo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32593/1/Agrobacterium-mediated.pdf
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Marc: |
LEADER 02225naa a2200205 a 4500
001 1488374
005 2015-11-23
008 2004 bl uuuu u00u1 u #d
100 1 $aCARVALHO, C. H. S.
245 $aAgrobacterium-mediated transformation of sorghum$bfactors that affect transformation efficiency.$h[electronic resource]
260 $c2004
520 $aThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection.
650 $aSorgo
700 1 $aZEHR, U. B.
700 1 $aGUNARATNA, N.
700 1 $aANDERSON, J.
700 1 $aKONONOWICZ, H. H.
700 1 $aHODGES, T. K.
700 1 $aAXTELL, J. D.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 27, n. 2, p. 259-269, 2004.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
19/10/2016 |
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Data da última atualização: |
19/10/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DEMATTÊ, J. A. M.; TERRA, F. da S.; OTTO, R.; TOMA, R. S.; PEREIRA, L. H.; NASCIMENTO, A. F. do; BORTOLETTO, M. A. M. |
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Afiliação: |
José A. M. Demattê, USP-ESALQ; Fabrício da Silva Terra, UFVJM; Rafael Otto, USP-ESALQ; Raul Shiso Toma, UFC; Luiz Henrique Pereira, UNESP-IGCE; ALEXANDRE FERREIRA DO NASCIMENTO, CPAMT; Marco Antonio Melo Bortoletto, GRUPO CLEALCO. |
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Título: |
Assessment of sugarcane harvesting residue effects on soil spectral behavior |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Scientia Agricola, Piracicaba, v. 73, n. 2, p. 159-168, mar./abr., 2016 |
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ISSN: |
0103-9016 |
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DOI: |
http://dx.doi.org/10.1590/0103-9016-2014-0370 |
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Idioma: |
Português |
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Conteúdo: |
When the harvesting of sugarcane involves a mechanized process, plant residues remain on the soil surface, which makes proximal and remote sensing difficult to monitor. This study aimed to evaluate, under laboratory conditions, differences in the soil spectral behavior of surface layers Quartzipsamment and Hapludox soil classes due to increasing levels of sugarcane?s dry (DL) and green (GL) leaf cover on the soil. Soil cover was quantified by supervised classification of the digital images (photography) taken of the treatments. The spectral reflectance of the samples was obtained using the FieldSpec Pro (350 to 2500 nm). TM-Landsat bands were simulated and the Normalized Difference Vegetation Index (NDVI) and soil line were also determined. Soil cover ranged from 0 to 89 % for DL and 0 to 80 % for GL. Dry leaf covering affected the features of the following soil constituents: iron oxides (480, 530 and 900 nm) and kaolinite (2200 nm). Water absorption (1400 and 1900 nm) and chlorophyll (670 nm) were determinant in differentiating between bare soil and GL covering. Bands 3 and 4 and NDVI showed pronounced variations as regards differences in soil cover percentage for both DL and GL. The soil line allowed for discrimination of the bare soil from the covered soil (DL and GL). High resolution sensors from about 50 % of the DL or GL covering are expected to reveal differences in soil spectral behavior. Above this coverage percentage, soil assessment by remote sensing is impaired. |
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Palavras-Chave: |
NDVI; Soil line; Soil proximal sensing; Soil remote sensing; TM-Landsat. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148949/1/2016-cpamt-nascimento-surgane-residue-effects-soil.pdf
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Marc: |
LEADER 02328naa a2200277 a 4500
001 2055007
005 2016-10-19
008 2016 bl uuuu u00u1 u #d
022 $a0103-9016
024 7 $ahttp://dx.doi.org/10.1590/0103-9016-2014-0370$2DOI
100 1 $aDEMATTÊ, J. A. M.
245 $aAssessment of sugarcane harvesting residue effects on soil spectral behavior$h[electronic resource]
260 $c2016
520 $aWhen the harvesting of sugarcane involves a mechanized process, plant residues remain on the soil surface, which makes proximal and remote sensing difficult to monitor. This study aimed to evaluate, under laboratory conditions, differences in the soil spectral behavior of surface layers Quartzipsamment and Hapludox soil classes due to increasing levels of sugarcane?s dry (DL) and green (GL) leaf cover on the soil. Soil cover was quantified by supervised classification of the digital images (photography) taken of the treatments. The spectral reflectance of the samples was obtained using the FieldSpec Pro (350 to 2500 nm). TM-Landsat bands were simulated and the Normalized Difference Vegetation Index (NDVI) and soil line were also determined. Soil cover ranged from 0 to 89 % for DL and 0 to 80 % for GL. Dry leaf covering affected the features of the following soil constituents: iron oxides (480, 530 and 900 nm) and kaolinite (2200 nm). Water absorption (1400 and 1900 nm) and chlorophyll (670 nm) were determinant in differentiating between bare soil and GL covering. Bands 3 and 4 and NDVI showed pronounced variations as regards differences in soil cover percentage for both DL and GL. The soil line allowed for discrimination of the bare soil from the covered soil (DL and GL). High resolution sensors from about 50 % of the DL or GL covering are expected to reveal differences in soil spectral behavior. Above this coverage percentage, soil assessment by remote sensing is impaired.
653 $aNDVI
653 $aSoil line
653 $aSoil proximal sensing
653 $aSoil remote sensing
653 $aTM-Landsat
700 1 $aTERRA, F. da S.
700 1 $aOTTO, R.
700 1 $aTOMA, R. S.
700 1 $aPEREIRA, L. H.
700 1 $aNASCIMENTO, A. F. do
700 1 $aBORTOLETTO, M. A. M.
773 $tScientia Agricola, Piracicaba$gv. 73, n. 2, p. 159-168, mar./abr., 2016
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Embrapa Agrossilvipastoril (CPAMT) |
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