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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
24/03/2008 |
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Data da última atualização: |
22/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; BIASE, F. H.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. |
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Afiliação: |
Felipe Perecin, USP/Pirassununga; SIMONE CRISTINA MEO NICIURA, CPPSE; Walt Yamazaki, Biotecnologia da Reprodução Animal Ltda; Christina Ramires Ferreira, Unicamp; Fernando Henrique Biase, USP/Pirassununga; Giovana Krentel Merighe, USP/Pirassununga; Flávio V. Meirelles, USP/Pirassununga; Joaquim Mansano Garcia, UNESP/Jaboticabal. |
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Título: |
Imprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Some gestational alterations associated with bovine somatic cell nuclear transfer (SCNT) are presumably consequences of abnormal imprinted gene expression. This work aimed to evaluate the expression patterns of imprinted genes IGF2 and IGF2R in bovine fetuses and chorioallantoic membranes derived from in vivo- and in vitro-produced embryos. Fetuses were produced by AI (in vivo group, n = 3), IVF (n = 3), parthenogenesis (n = 3), or SCNT (n = 2). Cows with positive pregnancy diagnosis after ultrasonographic examination were slaughtered between Days 33 and 36 of gestation. The reproductive tract was transported on ice to the laboratory, where fetuses and chorioallantoic fragments were collected and stored in liquid nitrogen. Total RNA extraction was performed using TRIzol, according to manufacturer's instructions, and the reverse transcription reaction was carried out with 1 µg of total RNA, 6.75 µm oligo pd(T)12?18, and 50 U of reverse transcriptase (Improm-II, Promega, Madison, WI, USA). The relative quantification of IGF2 and IGF2R transcripts was done using real-time PCR with SYBR Green dye. The average efficiency of PCR amplifications was estimated for each gene using a linear regression on the logarithm of fluorescence per cycle (Ramakers et al. 2003 Neurosci. Lett. 339, 62?66), and the expression ratios were calculated according to the method described previously by Livak and Schmittgen (2001 Methods 25, 402?408). To verify statistical differences, a pair-wise fixed reallocation randomization test (Pfaffl et al. 2002 Nucl. Acids Res. 30, e36) was used. All expression ratios were normalized by glyceraldehyde 3-phosphate dehydrogenase expression and calibrated by the in vivo group (expression assumed as 1.00 for all genes and tissues). The analysis of relative differences on transcript levels of imprinted genes in fetuses revealed IGF2 down-regulation (P < 0.05) in the SCNT (0.19) and parthenogenetic (0.02) groups when compared to the in vivo group and IVF fetuses (2.02). In chorioallantois, IGF2 was down-regulated (P < 0.001) in parthenotes (0.001) when compared to the in vivo, IVF (3.13), and SCNT (0.98) groups. IGF2R was down-regulated (P < 0.001) in SCNT chorioallantois (0.25) when compared to the in vivo group. Low expression of IGF2 in parthenogenetic fetuses and chorioallantois confirms its imprinted status in bovine. Alterations in the relative frequency of IGF2 and IGF2R transcripts were observed in bovine SCNT-derived fetuses and chorioallantoic membranes, respectively, supporting the hypothesis that abnormalities in the expression of imprinted genes are causes for the low efficiency of SCNT procedures in this species. Such alterations suggest modifications in DNA methylation patterns at IGF2 and IGF2R imprinting centers. MenosSome gestational alterations associated with bovine somatic cell nuclear transfer (SCNT) are presumably consequences of abnormal imprinted gene expression. This work aimed to evaluate the expression patterns of imprinted genes IGF2 and IGF2R in bovine fetuses and chorioallantoic membranes derived from in vivo- and in vitro-produced embryos. Fetuses were produced by AI (in vivo group, n = 3), IVF (n = 3), parthenogenesis (n = 3), or SCNT (n = 2). Cows with positive pregnancy diagnosis after ultrasonographic examination were slaughtered between Days 33 and 36 of gestation. The reproductive tract was transported on ice to the laboratory, where fetuses and chorioallantoic fragments were collected and stored in liquid nitrogen. Total RNA extraction was performed using TRIzol, according to manufacturer's instructions, and the reverse transcription reaction was carried out with 1 µg of total RNA, 6.75 µm oligo pd(T)12?18, and 50 U of reverse transcriptase (Improm-II, Promega, Madison, WI, USA). The relative quantification of IGF2 and IGF2R transcripts was done using real-time PCR with SYBR Green dye. The average efficiency of PCR amplifications was estimated for each gene using a linear regression on the logarithm of fluorescence per cycle (Ramakers et al. 2003 Neurosci. Lett. 339, 62?66), and the expression ratios were calculated according to the method described previously by Livak and Schmittgen (2001 Methods 25, 402?408). To verify statistical differences, a pair-wise fixed rea... Mostrar Tudo |
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Palavras-Chave: |
In vivo-and in vitro; Placentas; Produced bovine. |
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Thesaurus Nal: |
gene expression. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42088/1/PROCI-2008.00004.pdf
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Marc: |
LEADER 03522nam a2200241 a 4500
001 1048224
005 2023-06-22
008 2008 bl uuuu u00u1 u #d
100 1 $aPERECIN, F.
245 $aImprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas.$h[electronic resource]
260 $aReproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008.$c2008
520 $aSome gestational alterations associated with bovine somatic cell nuclear transfer (SCNT) are presumably consequences of abnormal imprinted gene expression. This work aimed to evaluate the expression patterns of imprinted genes IGF2 and IGF2R in bovine fetuses and chorioallantoic membranes derived from in vivo- and in vitro-produced embryos. Fetuses were produced by AI (in vivo group, n = 3), IVF (n = 3), parthenogenesis (n = 3), or SCNT (n = 2). Cows with positive pregnancy diagnosis after ultrasonographic examination were slaughtered between Days 33 and 36 of gestation. The reproductive tract was transported on ice to the laboratory, where fetuses and chorioallantoic fragments were collected and stored in liquid nitrogen. Total RNA extraction was performed using TRIzol, according to manufacturer's instructions, and the reverse transcription reaction was carried out with 1 µg of total RNA, 6.75 µm oligo pd(T)12?18, and 50 U of reverse transcriptase (Improm-II, Promega, Madison, WI, USA). The relative quantification of IGF2 and IGF2R transcripts was done using real-time PCR with SYBR Green dye. The average efficiency of PCR amplifications was estimated for each gene using a linear regression on the logarithm of fluorescence per cycle (Ramakers et al. 2003 Neurosci. Lett. 339, 62?66), and the expression ratios were calculated according to the method described previously by Livak and Schmittgen (2001 Methods 25, 402?408). To verify statistical differences, a pair-wise fixed reallocation randomization test (Pfaffl et al. 2002 Nucl. Acids Res. 30, e36) was used. All expression ratios were normalized by glyceraldehyde 3-phosphate dehydrogenase expression and calibrated by the in vivo group (expression assumed as 1.00 for all genes and tissues). The analysis of relative differences on transcript levels of imprinted genes in fetuses revealed IGF2 down-regulation (P < 0.05) in the SCNT (0.19) and parthenogenetic (0.02) groups when compared to the in vivo group and IVF fetuses (2.02). In chorioallantois, IGF2 was down-regulated (P < 0.001) in parthenotes (0.001) when compared to the in vivo, IVF (3.13), and SCNT (0.98) groups. IGF2R was down-regulated (P < 0.001) in SCNT chorioallantois (0.25) when compared to the in vivo group. Low expression of IGF2 in parthenogenetic fetuses and chorioallantois confirms its imprinted status in bovine. Alterations in the relative frequency of IGF2 and IGF2R transcripts were observed in bovine SCNT-derived fetuses and chorioallantoic membranes, respectively, supporting the hypothesis that abnormalities in the expression of imprinted genes are causes for the low efficiency of SCNT procedures in this species. Such alterations suggest modifications in DNA methylation patterns at IGF2 and IGF2R imprinting centers.
650 $agene expression
653 $aIn vivo-and in vitro
653 $aPlacentas
653 $aProduced bovine
700 1 $aNICIURA, S. C. M.
700 1 $aYAMAZAKI, W.
700 1 $aFERREIRA, C. R.
700 1 $aBIASE, F. H.
700 1 $aMERIGHE, G. K. F.
700 1 $aMEIRELLES, F. V.
700 1 $aGARCIA, J. M.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical; Embrapa Algodão. |
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Data corrente: |
03/01/2018 |
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Data da última atualização: |
17/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SANTOS, T. M.; SOUZA FILHO, M. de S. M. de; MUNIZ, C. R.; MORAIS, J. P. S.; KOTZEBUE, L. R. V.; PEREIRA, A. L. S.; AZEREDO, H. M. C. de. |
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Afiliação: |
TALITA M. SANTOS, UFCE; MEN DE SA MOREIRA DE SOUZA FILHO, CNPAT; CELLI RODRIGUES MUNIZ, CNPAT; JOAO PAULO SARAIVA MORAIS, CNPA; LLOYD RYAN VIANA KOTZEBUE, UFCE; ANDRÉ LUÍS SOUSA PEREIRA, UFCE; HENRIETTE MONTEIRO C DE AZEREDO, CNPAT. |
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Título: |
Zein films with unoxidized or oxidized tannic acid. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of the Science of Food and Agriculture, v. 97, n. 13, p. 4580-4587, 2017. |
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DOI: |
10.1002/jsfa.8327 |
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Idioma: |
Inglês |
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Conteúdo: |
Corn zein is apredominatlyhydrophobicprotein, formingfilmswith relativelygoodwater resistance.Tannic acid, especially in its oxidized form, is supposed to cross-link proteins including zein, which may be explored to further enhance the water resistance of zein films. The effects of different contents (0-8 wt%) of unoxidized and oxidized tannic acid (uTA and oTA, respectively) on the properties of zein films at different pH values (4-9) were studied, according to central composite designs. Increasing tannic acid contents and pH values resulted in decreased water solubility and increased tensile strength andmodulus of films. The presence of tannic acid provided the films with a yellowish color and increased opacity. Paired t-tests indicated that oTA films presented higher tensile strength, lower water vapor permeability and lower water solubility than uTA films. Higher tannic acid contents and pH values resulted in films with better overall physical properties, which might be ascribed to cross-linking, although the films were still not water resistant. The resulting films have potential to be used for food packaging and coating applications. |
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Palavras-Chave: |
Ácidos fenólicos; Biodegradable films; Cross-linking; Filme biodegradável; Filmes biodegradáveis; Protein; Tannic acid. |
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Thesagro: |
Ácido tânico; Cruzamento; Proteína; Retículo. |
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Thesaurus NAL: |
Phenolic acids. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171179/1/ART17065.pdf
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Marc: |
LEADER 02157naa a2200349 a 4500
001 2084074
005 2018-01-17
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1002/jsfa.8327$2DOI
100 1 $aSANTOS, T. M.
245 $aZein films with unoxidized or oxidized tannic acid.$h[electronic resource]
260 $c2017
520 $aCorn zein is apredominatlyhydrophobicprotein, formingfilmswith relativelygoodwater resistance.Tannic acid, especially in its oxidized form, is supposed to cross-link proteins including zein, which may be explored to further enhance the water resistance of zein films. The effects of different contents (0-8 wt%) of unoxidized and oxidized tannic acid (uTA and oTA, respectively) on the properties of zein films at different pH values (4-9) were studied, according to central composite designs. Increasing tannic acid contents and pH values resulted in decreased water solubility and increased tensile strength andmodulus of films. The presence of tannic acid provided the films with a yellowish color and increased opacity. Paired t-tests indicated that oTA films presented higher tensile strength, lower water vapor permeability and lower water solubility than uTA films. Higher tannic acid contents and pH values resulted in films with better overall physical properties, which might be ascribed to cross-linking, although the films were still not water resistant. The resulting films have potential to be used for food packaging and coating applications.
650 $aPhenolic acids
650 $aÁcido tânico
650 $aCruzamento
650 $aProteína
650 $aRetículo
653 $aÁcidos fenólicos
653 $aBiodegradable films
653 $aCross-linking
653 $aFilme biodegradável
653 $aFilmes biodegradáveis
653 $aProtein
653 $aTannic acid
700 1 $aSOUZA FILHO, M. de S. M. de
700 1 $aMUNIZ, C. R.
700 1 $aMORAIS, J. P. S.
700 1 $aKOTZEBUE, L. R. V.
700 1 $aPEREIRA, A. L. S.
700 1 $aAZEREDO, H. M. C. de
773 $tJournal of the Science of Food and Agriculture$gv. 97, n. 13, p. 4580-4587, 2017.
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Registro original: |
Embrapa Algodão (CNPA) |
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