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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/03/2006 |
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Data da última atualização: |
22/02/2016 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
RABELLO, A. R.; QUEIROZ, P. R.; SIMÕES, K. C.; HIRAGI, C. O.; LIMA, L. H. C.; OLIVEIRA, M. R. V.; MEHTA, A. |
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Título: |
Diferenciação de biótipos de Bemísia tabaci utilizando PCR-RFLP e sequenciamento da região ITS1 rDNA. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2005. |
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Páginas: |
23 p. |
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Série: |
(Embrapa Recursos Genéticos e Biotecnologia. Boletim de Pesquisa e Desenvolvimento, 95) |
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Idioma: |
Português |
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Palavras-Chave: |
Biótipos; Biotype; ITS1; PCR-RFLP; rDNA. |
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Thesagro: |
Bemisia Tabaci; Mosca Branca. |
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Thesaurus Nal: |
Bemisia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CENARGEN/26632/1/bp095.pdf
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Marc: |
LEADER 00888nam a2200289 a 4500
001 1187042
005 2016-02-22
008 2005 bl uuuu u0uu1 u #d
100 1 $aRABELLO, A. R.
245 $aDiferenciação de biótipos de Bemísia tabaci utilizando PCR-RFLP e sequenciamento da região ITS1 rDNA.$h[electronic resource]
260 $aBrasília: Embrapa Recursos Genéticos e Biotecnologia$c2005
300 $a23 p.
490 $a(Embrapa Recursos Genéticos e Biotecnologia. Boletim de Pesquisa e Desenvolvimento, 95)
650 $aBemisia
650 $aBemisia Tabaci
650 $aMosca Branca
653 $aBiótipos
653 $aBiotype
653 $aITS1
653 $aPCR-RFLP
653 $arDNA
700 1 $aQUEIROZ, P. R.
700 1 $aSIMÕES, K. C.
700 1 $aHIRAGI, C. O.
700 1 $aLIMA, L. H. C.
700 1 $aOLIVEIRA, M. R. V.
700 1 $aMEHTA, A.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/02/2008 |
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Data da última atualização: |
12/02/2008 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Nacional - A |
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Autoria: |
KITAJIMA, E. W.; GROOT, T. V. M.; NOVELLI, V. M.; FREITAS-ÁSTUA, J.; ALBERTI, G.; MORAES, G. J. de. |
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Afiliação: |
Elliot W. Kitajima, ESALQ; Thomas V. M. Groot, UvA; Valdenice M. Novelli, APTA; Juliana Freitas-Astúa, CNPMF; Gerd Alberti; Gilberto J. de Moraes, ESALQ. |
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Título: |
In situ observation of the Cardinium symbionts of Brevipalpus (Acari: Tenuipalpidae) by electron microscopy. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Experimental & Applied Acarology, v. 42, p. 263-271, 2007. |
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Idioma: |
Português |
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Conteúdo: |
Brevipalpus (Acari: Tenuipalpidae) mites are important pests on a variety of host plant species. The mites damage their hosts directly by feeding and some species also serve as vectors of plant viruses. Among more than 200 described Brevipalpus species, three are recognized as vectors of plant viruses: B. phoenicis, B. californicus and B. obovatus. These species occur worldwide in subtropical and tropical regions. Brevipalpus mites reproduce mostly by thelytokous parthenogenesis and this condition was attributed to a bacterial endosymbiont, recently characterized as a member of the genus Cardinium. The same symbiont infects many other arthropods and is capable of manipulating their host reproduction in various ways. Generally the presence of Cardinium is determined by molecular, PCR based, techniques. In the current work we present visual evidence for the presence of these bacteria by transmission electron microscopy as a complement of previous detection by PCR. Cardinium is easily identified by the presence of a unique array of microtubule-like structures (ML) in the cell. Symbionts have been observed in several organs and eggs from different populations of all three Brevipalpus species known as vector of plant viruses. Cardinium cells were always immersed directly within the cytoplasm of infected cells. Bacteria were observed in all females of all instars, but were absent from all males examined. Females from some Brevipalpus populations were observed to be uninfected by Cardinium. This observation confirmed previous PCR-based results that these populations were aposymbiotic. The observed distribution of the bacteria suggests that these bacteria could have other functions in the mite biology beside feminization. MenosBrevipalpus (Acari: Tenuipalpidae) mites are important pests on a variety of host plant species. The mites damage their hosts directly by feeding and some species also serve as vectors of plant viruses. Among more than 200 described Brevipalpus species, three are recognized as vectors of plant viruses: B. phoenicis, B. californicus and B. obovatus. These species occur worldwide in subtropical and tropical regions. Brevipalpus mites reproduce mostly by thelytokous parthenogenesis and this condition was attributed to a bacterial endosymbiont, recently characterized as a member of the genus Cardinium. The same symbiont infects many other arthropods and is capable of manipulating their host reproduction in various ways. Generally the presence of Cardinium is determined by molecular, PCR based, techniques. In the current work we present visual evidence for the presence of these bacteria by transmission electron microscopy as a complement of previous detection by PCR. Cardinium is easily identified by the presence of a unique array of microtubule-like structures (ML) in the cell. Symbionts have been observed in several organs and eggs from different populations of all three Brevipalpus species known as vector of plant viruses. Cardinium cells were always immersed directly within the cytoplasm of infected cells. Bacteria were observed in all females of all instars, but were absent from all males examined. Females from some Brevipalpus populations were observed to be uninfected by C... Mostrar Tudo |
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Palavras-Chave: |
Aposymbiont; B californicus; B obovatus; B phoenicis; californicus; False spider mites; obovatus; phoenicis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02523naa a2200277 a 4500
001 1654618
005 2008-02-12
008 2007 bl --- 0-- u #d
100 1 $aKITAJIMA, E. W.
245 $aIn situ observation of the Cardinium symbionts of Brevipalpus (Acari$bTenuipalpidae) by electron microscopy.
260 $c2007
520 $aBrevipalpus (Acari: Tenuipalpidae) mites are important pests on a variety of host plant species. The mites damage their hosts directly by feeding and some species also serve as vectors of plant viruses. Among more than 200 described Brevipalpus species, three are recognized as vectors of plant viruses: B. phoenicis, B. californicus and B. obovatus. These species occur worldwide in subtropical and tropical regions. Brevipalpus mites reproduce mostly by thelytokous parthenogenesis and this condition was attributed to a bacterial endosymbiont, recently characterized as a member of the genus Cardinium. The same symbiont infects many other arthropods and is capable of manipulating their host reproduction in various ways. Generally the presence of Cardinium is determined by molecular, PCR based, techniques. In the current work we present visual evidence for the presence of these bacteria by transmission electron microscopy as a complement of previous detection by PCR. Cardinium is easily identified by the presence of a unique array of microtubule-like structures (ML) in the cell. Symbionts have been observed in several organs and eggs from different populations of all three Brevipalpus species known as vector of plant viruses. Cardinium cells were always immersed directly within the cytoplasm of infected cells. Bacteria were observed in all females of all instars, but were absent from all males examined. Females from some Brevipalpus populations were observed to be uninfected by Cardinium. This observation confirmed previous PCR-based results that these populations were aposymbiotic. The observed distribution of the bacteria suggests that these bacteria could have other functions in the mite biology beside feminization.
653 $aAposymbiont
653 $aB californicus
653 $aB obovatus
653 $aB phoenicis
653 $acalifornicus
653 $aFalse spider mites
653 $aobovatus
653 $aphoenicis
700 1 $aGROOT, T. V. M.
700 1 $aNOVELLI, V. M.
700 1 $aFREITAS-ÁSTUA, J.
700 1 $aALBERTI, G.
700 1 $aMORAES, G. J. de
773 $tExperimental & Applied Acarology$gv. 42, p. 263-271, 2007.
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