|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
16/09/1999 |
|
Data da última atualização: |
16/09/1999 |
|
Autoria: |
CHAVARRIAGA-Aguirre, P.; MAYA, M. M.; BONIERBALE, M. W.; KRESOVICH, S.; FREGENE, M. A.; TOHME, J.; KOCHERT, G. |
|
Afiliação: |
Department of Botany, University of Georgia, Athens GA 30605, USA; Cassava Program, CIAT, A.A. 6713, Cali, Colombia; Merideth Bonierbale, International Potato Center (CIP), Apartado 1558, Lima 12, Peru. |
|
Título: |
Microsatellites in Cassava (Manihot esculenta Crantz): discovery, inheritance and variability |
|
Ano de publicação: |
1998 |
|
Fonte/Imprenta: |
Theor Appl Genet, v.97, p.493-501, 1998 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Fourteen microsatellites containing GA-repeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (32P)-end-labeled primers and in more than 500 accessions using fluorescence-based genotyping. Heterozygosity values ranged from 0.00 to 0.88 and the number of alleles detected varied from 1 to 15. The reproducibility of allele sizing was also assessed using fluorescence-based genotyping. The average inter-gel size difference was 1.03 nucleotides. Chi-square tests (%2) were performed to analyse segregation distortion and the linkage between alleles segregating from either or both parents in an F1 mapping population. |
|
Palavras-Chave: |
Fluorescence-based genotyping; Linkage; Microsatellites. |
|
Thesaurus Nal: |
cassava; heterozygosity. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01425naa a2200253 a 4500
001 1648293
005 1999-09-16
008 1998 bl --- 0-- u #d
100 1 $aCHAVARRIAGA-Aguirre, P.
245 $aMicrosatellites in Cassava (Manihot esculenta Crantz)$bdiscovery, inheritance and variability
260 $c1998
520 $aFourteen microsatellites containing GA-repeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (32P)-end-labeled primers and in more than 500 accessions using fluorescence-based genotyping. Heterozygosity values ranged from 0.00 to 0.88 and the number of alleles detected varied from 1 to 15. The reproducibility of allele sizing was also assessed using fluorescence-based genotyping. The average inter-gel size difference was 1.03 nucleotides. Chi-square tests (%2) were performed to analyse segregation distortion and the linkage between alleles segregating from either or both parents in an F1 mapping population.
650 $acassava
650 $aheterozygosity
653 $aFluorescence-based genotyping
653 $aLinkage
653 $aMicrosatellites
700 1 $aMAYA, M. M.
700 1 $aBONIERBALE, M. W.
700 1 $aKRESOVICH, S.
700 1 $aFREGENE, M. A.
700 1 $aTOHME, J.
700 1 $aKOCHERT, G.
773 $tTheor Appl Genet$gv.97, p.493-501, 1998
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
