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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
29/12/2020 |
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Data da última atualização: |
29/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, L. J. da; CREVELIN, E. J.; SOUZA, D. T.; LACERDA JÚNIOR, G. V.; OLIVEIRA, V. M.; RUIZ, A. L. T. G.; ROSA, L. H.; MORAES, L. A. B.; MELO, I. S. de. |
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Afiliação: |
LEONARDO JOSÉ SILVA, ESALQ-USP; EDUARDO JOSÉ CREVELIN, FFCLRP-USP; DANILO TOSTA SOUZA, FFCLRP-USP; GILENO VIEIRA LACERDA JÚNIOR; VALERIA MAIA DE OLIVEIRA, CPQBA-UNICAMP; ANA LUCIA TASCA GOIS RUIZ, FCF-UNICAMP; LUIZ HENRIQUE ROSA, UFMG; LUIZ ALBERTO BERALDO MORAES, FFCLRP-USP; ITAMAR SOARES DE MELO, CNPMA. |
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Título: |
Actinobacteria from Antarctica as a source for anticancer discovery. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Scientific Reports, v. 10, article 13870, 2020. |
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Páginas: |
p. 1-15. |
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ISSN: |
2045-2322 |
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DOI: |
https://doi.org/10.1038/s41598-020-69786-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Although many advances have been achieved to treat aggressive tumours, cancer remains a leading cause of death and a public health problem worldwide. Among the main approaches for the discovery of new bioactive agents, the prospect of microbial secondary metabolites represents an effective source for the development of drug leads. In this study, we investigated the actinobacterial diversity associated with an endemic Antarctic species, Deschampsia antarctica, by integrated culture-dependent and culture-independent methods and acknowledged this niche as a reservoir of bioactive strains for the production of antitumour compounds. The 16S rRNA-based analysis showed the predominance of the Actinomycetales order, a well-known group of bioactive metabolite producers belonging to the Actinobacteria phylum. Cultivation techniques were applied, and 72 psychrotolerant Actinobacteria strains belonging to the genera Actinoplanes, Arthrobacter, Kribbella, Mycobacterium, Nocardia, Pilimelia, Pseudarthrobacter, Rhodococcus, Streptacidiphilus, Streptomyces and Tsukamurella were identified. The secondary metabolites were screened, and 17 isolates were identified as promising antitumour compound producers. However, the bio-guided assay showed a pronounced antiproliferative activity for the crude extracts of Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653. The TGI and LC50 values revealed the potential of these natural products to control the proliferation of breast (MCF-7), glioblastoma (U251), lung/non-small (NCI-H460) and kidney (786-0) human cancer cell lines. Cinerubin B and actinomycin V were the predominant compounds identified in Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653, respectively. Our results suggest that the rhizosphere of D. antarctica represents a prominent reservoir of bioactive actinobacteria strains and reveals it as an important environment for potential antitumour agents. MenosAbstract: Although many advances have been achieved to treat aggressive tumours, cancer remains a leading cause of death and a public health problem worldwide. Among the main approaches for the discovery of new bioactive agents, the prospect of microbial secondary metabolites represents an effective source for the development of drug leads. In this study, we investigated the actinobacterial diversity associated with an endemic Antarctic species, Deschampsia antarctica, by integrated culture-dependent and culture-independent methods and acknowledged this niche as a reservoir of bioactive strains for the production of antitumour compounds. The 16S rRNA-based analysis showed the predominance of the Actinomycetales order, a well-known group of bioactive metabolite producers belonging to the Actinobacteria phylum. Cultivation techniques were applied, and 72 psychrotolerant Actinobacteria strains belonging to the genera Actinoplanes, Arthrobacter, Kribbella, Mycobacterium, Nocardia, Pilimelia, Pseudarthrobacter, Rhodococcus, Streptacidiphilus, Streptomyces and Tsukamurella were identified. The secondary metabolites were screened, and 17 isolates were identified as promising antitumour compound producers. However, the bio-guided assay showed a pronounced antiproliferative activity for the crude extracts of Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653. The TGI and LC50 values revealed the potential of these natural products to control the proliferation of breast (MCF-7)... Mostrar Tudo |
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Palavras-Chave: |
Anticancer activity; Antitumoral activity. |
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Thesagro: |
Bactéria não Patogênica; Câncer. |
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Thesaurus Nal: |
Actinobacteria; Antarctica. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219757/1/Melo-Actinobacteria-Antarctica-2020.pdf
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Marc: |
LEADER 02877naa a2200325 a 4500
001 2128824
005 2020-12-29
008 2020 bl uuuu u00u1 u #d
022 $a2045-2322
024 7 $ahttps://doi.org/10.1038/s41598-020-69786-2$2DOI
100 1 $aSILVA, L. J. da
245 $aActinobacteria from Antarctica as a source for anticancer discovery.$h[electronic resource]
260 $c2020
300 $ap. 1-15.
520 $aAbstract: Although many advances have been achieved to treat aggressive tumours, cancer remains a leading cause of death and a public health problem worldwide. Among the main approaches for the discovery of new bioactive agents, the prospect of microbial secondary metabolites represents an effective source for the development of drug leads. In this study, we investigated the actinobacterial diversity associated with an endemic Antarctic species, Deschampsia antarctica, by integrated culture-dependent and culture-independent methods and acknowledged this niche as a reservoir of bioactive strains for the production of antitumour compounds. The 16S rRNA-based analysis showed the predominance of the Actinomycetales order, a well-known group of bioactive metabolite producers belonging to the Actinobacteria phylum. Cultivation techniques were applied, and 72 psychrotolerant Actinobacteria strains belonging to the genera Actinoplanes, Arthrobacter, Kribbella, Mycobacterium, Nocardia, Pilimelia, Pseudarthrobacter, Rhodococcus, Streptacidiphilus, Streptomyces and Tsukamurella were identified. The secondary metabolites were screened, and 17 isolates were identified as promising antitumour compound producers. However, the bio-guided assay showed a pronounced antiproliferative activity for the crude extracts of Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653. The TGI and LC50 values revealed the potential of these natural products to control the proliferation of breast (MCF-7), glioblastoma (U251), lung/non-small (NCI-H460) and kidney (786-0) human cancer cell lines. Cinerubin B and actinomycin V were the predominant compounds identified in Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653, respectively. Our results suggest that the rhizosphere of D. antarctica represents a prominent reservoir of bioactive actinobacteria strains and reveals it as an important environment for potential antitumour agents.
650 $aActinobacteria
650 $aAntarctica
650 $aBactéria não Patogênica
650 $aCâncer
653 $aAnticancer activity
653 $aAntitumoral activity
700 1 $aCREVELIN, E. J.
700 1 $aSOUZA, D. T.
700 1 $aLACERDA JÚNIOR, G. V.
700 1 $aOLIVEIRA, V. M.
700 1 $aRUIZ, A. L. T. G.
700 1 $aROSA, L. H.
700 1 $aMORAES, L. A. B.
700 1 $aMELO, I. S. de
773 $tScientific Reports$gv. 10, article 13870, 2020.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
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| Registros recuperados : 16 | |
| 3. |  | SILVA, L. J. da; CREVELIN, E. J.; SOUZA, D. T.; LACERDA JÚNIOR, G. V.; OLIVEIRA, V. M.; RUIZ, A. L. T. G.; ROSA, L. H.; MORAES, L. A. B.; MELO, I. S. de. Actinobacteria from Antarctica as a source for anticancer discovery. Scientific Reports, v. 10, article 13870, 2020. p. 1-15.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 4. | | PASSOS, L. P.; KOPP, M. M.; VIDIGAL, M. C.; FERNANDES, F. DE S.; MARQUES, R.; VALE, N. M. DO; BARILI, L. D. Assessment of the efficiency of disinfectants and their influence on the germination of Brachiaria seeds for in vitro culture purposes. In: INTERNATIONAL ANNUAL MEETINGS, 2009, Pittsburgh. Anais... Pittsburg: ASA-CSSA-SSSA, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | COSTA, N. de L.; PAULINO, V. T.; MAGALHÃES, J. A; TOWNSEND, C. R.; PEREIRA, R. G. de A. Análise do crescimento de Brachiaria dictyoneura nos cerrados de Rondônia. PUBVET, Londrina, v. 7, n. 22, Ed. 245, Art. 1618, nov. 2013. Não paginado.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Rondônia; Embrapa Roraima. |
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| 7. | | CONGRO, C. R.; GIUMELLI, O. D.; FACCENDA, O.; SOUZA, M. J. B. de. Avaliação da qualidade dos serviços turísticos da capital do Pantanal Sul: Corumbá (MS). Separata de Evidência, Unoesc, v. 5, n.2, p. 363-378, jul./dez., 2005. 16 p.| Biblioteca(s): Embrapa Pantanal. |
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| 8. | | STAUT, L. A.; KURIHARA, C. H. Calagem e adubacao. In: EMBRAPA AGROPECUARIA OESTE. Algodao: tecnologia de producao. Dourados: Embrapa Agropecuaria Oeste; Campina Gradne: Embrapa Algodao, 2001. p.103-123.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 10. | | GOMES, E. A.; LANA, U. G. P.; GUIMARAES, C. T.; CARNEIRO, N. P.; JARDIM, S. N.; ALVES, V. M. C.; PURCINO, A. A. C. Genes induzidos por alumínio em apices de raízes de uma cultivar de arroz tolerante. In: CONGRESSO NACIONAL DE MILHO E SORGO, 25.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 1., 2004, Cuiabá, MT. Da agricultura familiar ao agronegócio: tecnologia, competitividade e sustentabilidade: [resumos expandidos]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Cuiabá: Empaer, 2004. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 11. | | MIRANDA, E. E. de (Coord.). Brasil Visto do Espaço: Pernambuco Campinas: Embrapa Monitoramento por Satélite; Brasília: Embrapa Comunicação para Transferência de Tecnologia, 2002. 1 CD-ROM; Windows 9x ou NT; Composição col. Coordenador técnico: Alexandre Camargo Coutinho; Equipe técnica: Carlos Fernando Assis Paniago, Marcelo Guimarães, José Roberto Miranda, Ana Lúcia Filardi.| Biblioteca(s): Embrapa Territorial. |
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| 12. | | FRANÇA-NETO, J. B.; KRZYZANOWSKI, F. C.; WEST, S. H.; HENNING, A. A.; COSTA, N. P. Comparacao da qualidade de sementes de soja com tegumento preto e amarelo. Informativo ABRATES, Curitiba, v.9, n.1/2, p.33, jul./ago. 1999. Numero especial, ref. 005. Resumo apresentado no XI Congresso Brasileiro de Sementes.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 13. | | NASCIMENTO, R. J. M.; BEZERRA, L. C. A.; ALMEIDA, J. S.; BARROS, M. DE O.; SILVA, L. R. R.; ROSA, M. de F.; MAZZETO, S. E.; LOMONACO, D.; PEREIRA, K. R. A.; AVELINO, F. Elucidating the adsorption mechanism of Rhodamine B on mesoporous coconut coir?based biosorbents through a non?linear modeling and recycling approach. Environmental Science and Pollution Research, jan. 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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